Ontogeny of salivary peptide P-C like immunoreactivity in human pancreatic B-cells

1983 ◽  
Vol 103 (4) ◽  
pp. 552-557 ◽  
Author(s):  
Seiki Ito ◽  
Satoko Isemura ◽  
Eiichi Saitoh ◽  
Kazuo Sanada ◽  
Toshimitsu Suzuki ◽  
...  
Keyword(s):  
B Cells ◽  
Insulin Release ◽  
Protein C ◽  
Calcium Concentration ◽  
Salivary Protein ◽  
Human Pancreas ◽  
Amino Acid Residues ◽  
Salivary Peptide ◽  
Pancreatic B Cells ◽  
Foetal Pancreas

Abstract. An immunohistochemical study using antisera against proline rich salivary peptide P-C and insulin, glucagon, somatostatin and pancreatic polypeptide antisera was carried out on the foetal pancreas at different stages and on the newborn infant's, infant's, child's and adult pancreas to examine the time at which salivary peptide P-C like immunoreactivity appeared in the human pancreas. Salivary peptide P-C like immunoreactive cells first appeared as a few scattered cells in the foetal pancreas after 16 weeks of gestation and gradually increased in numbers during gestation. The cells corresponded only to insulin immunoreactive cells in the foetal, newborn infant's, infant's, child's and adult pancreas. Only some of the insulin immunoreactive cells in the foetal pancreas contained salivary peptide P-C like immunoreactivity while the majority of those in the infant's pancreas and all those in the child's and adult pancreas did so. The findings, together with the fact that the full sequence of salivary peptide P-C is identical to the COOH-terminal 44 amino acid residues of Salivary Protein C, led to the possibility that peptide P-C like immunoreactivity in the human pancreatic B-cells was not a moiety of the precursor of insulin and pro-insulin, but a moiety of Salivary Protein C. It has been suggested that, in saliva, Salivary Protein C aids in maintenance of the calcium concentration. Based on the hypothesis that peptide P-C like immunoreactivity in the human pancreatic B-cells may play some role in insulin release through the maintenance of the calcium concentration, the present finding seems to explain the fact that the mechanism for insulin release in the foetal pancreas is immature in spite of sufficient biosynthesis of insulin.

Intracellular localization of salivary peptide P-C-like immunoreactivity in the human pancreatic B-cells

1985 ◽  
Vol 108 (1) ◽  
pp. 119-129 ◽  
Author(s):  
Seiki Ito ◽  
Toshimitsu Suzuki ◽  
Tooru Izumi ◽  
Takeshi Momotsu ◽  
Satoko Isemura ◽  
...  
Keyword(s):  
B Cells ◽  
Protein C ◽  
Immunohistochemical Study ◽  
Secretory Granules ◽  
Intracellular Localization ◽  
Salivary Protein ◽  
Antigenic Determinants ◽  
Electron Microscopic ◽  
Salivary Peptide ◽  
Pancreatic B Cells

Abstract. In order to clarify the intracellular localization of salivary peptide P-C-like immunoreactivity in human pancreatic B-cells, an immunohistochemical study at electron microscopic levels was carried out by the protein A-gold technique using antisera against insulin and salivary peptide P-C. Both salivary peptide P-C-like immunoreactivity and insulin-like immunoreactivity were present only in the insulin secretory granules of the pancreatic B-cells. However, the former immunoreactivity was lacking in many insulin secretory granules of foetal pancreatic B-cells while the latter immunoreactivity was seen in all insulin secretory granules. Salivary peptide P-C-like immunoreactivity was not found in the other kinds of cells in the islets. In a previous immunohistochemical study at light microscopic level, salivary peptide P-C-like immunoreactivity appeared in a few pancreatic B-cells at about the 16th week of gestation, in an increasing number during gestation, and was seen in all pancreatic B-cells a few months after birth. The present finding together with the above results suggest that absence of salivary peptide P-C-like immunoreactivity in some foetal pancreatic B-cells may be due to the underdevelopment of salivary peptide P-C-like immunoreactivity in each insulin secretory granule. From the examination of cross-reactivity of antisera against salivary peptide P-C to other kinds of salivary peptides and salivary Protein C, and from the results of an indirect immunofluorescence technique using three kinds of antisera including antisera against salivary peptide P-C, salivary peptide P-B and salivary Protein C, it was thought that salivary peptide P-C-like immunoreactivity in human pancreatic B-cells belongs neither to salivary Protein C nor to salivary peptide P-B nor to salivary peptide P-E, but either to salivary peptide P-C itself or to an unknown substance which has common antigenic determinants with salivary peptide P-C, salivary peptide P-B and salivary Protein C. Salivary peptide P-C-like immunoreactivity was not found in the pancreatic B-cells of other mammals. Thus, although a new substance other than insulin is present in the insulin secretory granules of the human pancreatic B-cells, its pathophysiological function remains unclear.

Role of CYP2E1 in ketone-stimulated insulin release in pancreatic B-cells

2004 ◽  
Vol 67 (5) ◽  
pp. 875-884 ◽  
Author(s):  
Diane J.Lees Murdock ◽  
Jacqueline Clarke ◽  
Peter R. Flatt ◽  
Yvonne A. Barnett ◽  
Christopher R. Barnett
Keyword(s):  
B Cells ◽  
Insulin Release ◽  
Pancreatic B Cells

scholarly journals Die ultrastrukturele, morfometriese evaluering van die mikrotubulere stelsel in gestimuleerde B-selle van die pankreas

1992 ◽  
Vol 11 (4) ◽  
pp. 127-135
Author(s):  
A. Crous ◽  
A. M. De Beer ◽  
E. J. Visser
Keyword(s):  
B Cells ◽  
Insulin Release ◽  
Insulin Response ◽  
High Magnification ◽  
Tissue Samples ◽  
Islet Tissue ◽  
Pancreatic B Cells ◽  
Complete Cell

The intracellular distribution of microtubules in pancreatic B-cells was studied morphometrically to elucidate the positive correlation between microtubular content and the rate of insulin release found by biochemical investigations. Rat islet tissue was glucose stimulated under in vivo and in vitro (isolated islets) conditions and tissue samples taken to represent both phases of the phasic insulin response. Electron micrographs (x40 000) of individual B-cells were assembled into montages to obtain complete cell profiles at high magnification.

Presence of salivary Protein C and salivary peptide P-C-like immunoreactivity in the laryngo-tracheo-bronchial glands

1985 ◽  
Vol 108 (1) ◽  
pp. 130-134 ◽  
Author(s):  
Seiki Ito ◽  
Toshimitsu Suzuki ◽  
Takeshi Momotsu ◽  
Satoko Isemura ◽  
Eiichi Saitoh ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Protein C ◽  
Human Saliva ◽  
Salivary Protein ◽  
Serial Sections ◽  
Immunofluorescence Technique ◽  
Salivary Peptide ◽  
Morphological Finding ◽  
Indirect Immunofluorescence Technique ◽  
Bronchial Glands

Abstract. An indirect immunofluorescence technique using antisera aganist salivary peptide P-C and against salivary Protein C was carried out on the laryngeal, tracheal and bronchial glands to examine whether salivary peptide P-C-like immunoreactivity, recently demonstrated in the serous cells of the human salivary glands, was also present in those of laryngeal, tracheal and bronchial glands and to ascertain whether salivary peptide P-C is a fragment of salivary Protein C or not. Salivary peptide P-C-like immunoreactivity was present in the serous cells of the human laryngeal, tracheal and bronchial glands. Observation of serial sections immunostained with two kinds of antisera revealed that cells reacting with antisera against salivary peptide P-C were identical to those reacting with antisera against salivary Protein C pre-incubated with salivary peptide P-C. The finding implied that salivary peptide P-C and salivary Protein C, originally isolated from human saliva, were also present in the serous cells of tissues other than the salivary glands. Furthermore, analysis of the primary structure of salivary peptide P-C and salivary Protein C together with the present morphological finding suggests that salivary peptide P-C is a COOH-terminal fragment of salivary Protein C. Thus, salivary Protein C and salivary peptide P-C may play some role in the function of the serous cells of the salivary and laryngo-tracheobronchial glands.

Pinacidil inhibits insulin release by increasing K+ outflow from pancreatic B-cells

1988 ◽  
Vol 156 (2) ◽  
pp. 283-286 ◽  
Author(s):  
Philippe Lebrun ◽  
Vincent Devreux ◽  
Marcel Hermann ◽  
André Herchuelz
Keyword(s):  
B Cells ◽  
Insulin Release ◽  
Pancreatic B Cells

Effects of islet hormones on insulin secretion from cloned B cell lines, HIT-T15 and RINm5F

1989 ◽  
Vol 121 (3) ◽  
pp. 479-485 ◽  
Author(s):  
D. G. Lambert ◽  
T. W. Atkins
Keyword(s):  
Insulin Secretion ◽  
B Cells ◽  
Insulin Release ◽  
Pancreatic Polypeptide ◽  
Islet Cell ◽  
Fine Tuning ◽  
Rinm5f Cells ◽  
Islet Hormones ◽  
Pancreatic B Cells ◽  
Islet Cell Hormones

ABSTRACT The effects of the islet cell hormones glucagon, somatostatin-28 and pancreatic polypeptide on insulin secretion from cultured cloned pancreatic B cells (HIT-T15 and RINm5F) have been investigated. Glucagon stimulates the secretion of insulin from HIT-T15 cells in the absence and presence of glucose and from RINm5F cells in the absence and presence of glyceraldehyde. HIT-T15 cells were more sensitive to the stimulatory effect of glucagon than RINm5F cells. Somatostatin-28 and pancreatic polypeptide, both alone and in combination, reduced glucose- and glucagon-stimulated insulin release from HIT-T15 cells and glyceraldehyde- and glucagon-stimulated insulin release from RINm5F cells. HIT-T15 cells were more sensitive to the inhibitory actions of somatostatin-28 and pancreatic polypeptide than RINm5F cells. This study supports the hypothesis that insulin release from normal B cells may be modified by the paracrine activity of islet hormones, glucagon, somatostatin and pancreatic polypeptide and probably occurs before any fine tuning imposed by subsequently released insulin. Journal of Endocrinology (1989) 121, 479–485

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