T lymphocyte activation in euthyroid Graves' ophthalmopathy: soluble interleukin 2 receptor release, cellular interleukin 2 receptor expression and interleukin 2 production

1989 ◽  
Vol 120 (5) ◽  
pp. 602-609 ◽  
Author(s):  
Kar Neng Lai ◽  
Joseph C. K. Leung ◽  
Chun Chung Chow ◽  
Clive S. Cockram
Keyword(s):  
T Lymphocyte ◽  
Interleukin 2 ◽  
Lymphocyte Activation ◽  
Receptor Expression ◽  
Pokeweed Mitogen ◽  
Healthy Controls ◽  
Interleukin 2 Receptor ◽  
T Lymphocyte Activation ◽  
Graves Ophthalmopathy ◽  
Soluble Interleukin 2 Receptor

Abstract. The present study was undertaken to examine the cellular control arm of the immune response with regard to T lymphocyte proliferation in euthyroid Graves' ophthalmopathy. Twenty patients with euthyroid Graves' ophthalmopathy (7 on antithyroid drugs and 13 on no treatment) and 18 healthy controls were studied in an infection-free period. Mitogen-stimulated cellular interleukin 2 (IL2) receptor expression, soluble interleukin 2 receptor release, and interleukin 2 production, were studied in peripheral blood mononuclear cells cultured for 24 h. The cellular IL2 receptor expression and soluble IL2 receptor release did not differ between the patients and healthy controls. In contrast, IL2 production in response to pokeweed mitogen stimulation was increased in lymphocytes from patients with Graves' ophthalmopathy. The IL2 release did not correlate with the quantities of cellular and soluble IL2 receptor. The mitogen-stimulated cellular IL2 receptor expression, IL2 receptor release, and IL2 production did not differ between patients with or without carbimazole therapy. Despite a suggested role of autoreactive T cells in mediating the development and propagation of autoimmune thyroid disease, this study fails to demonstrate a defective T lymphocyte activation state in patients with Graves' ophthalmopathy during an euthyroid state.

scholarly journals Correlation Between Thymoma and Soluble Interleukin-2 Receptor Expression in a Patient with Good Syndrome

2021 ◽  
Vol Volume 14 ◽  
pp. 5045-5049
Author(s):  
Haruka Kitano ◽  
Fumihiro Yamaguchi ◽  
Kenji Atarashi ◽  
Mina Hiraiwa ◽  
Yo Shiratori ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Receptor Expression ◽  
Interleukin 2 Receptor ◽  
Good Syndrome ◽  
Soluble Interleukin 2 Receptor

scholarly journals Isolation and structural characterization of the human 4F2 heavy-chain gene, an inducible gene involved in T-lymphocyte activation.

1988 ◽  
Vol 8 (9) ◽  
pp. 3809-3819 ◽  
Author(s):  
K M Gottesdiener ◽  
B A Karpinski ◽  
T Lindsten ◽  
J L Strominger ◽  
N H Jones ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Surface ◽  
T Lymphocyte ◽  
Heavy Chain ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Lymphocyte Activation ◽  
Upstream Region ◽  
Activated T Cells ◽  
T Lymphocyte Activation

The human 4F2 cell surface antigen is a 120-kilodalton (kDa) disulfide-linked heterodimer which is composed of an 80- to 90-kDa glycosylated heavy chain (4F2HC) and a 35- to 40-kDa nonglycosylated light chain (4F2LC). 4F2 belongs to a family of inducible cell surface molecules which are involved in T-lymphocyte activation and growth. To better understand the molecular mechanism(s) that controls 4F2HC gene expression in both resting and activated T cells, a 4F2HC human genomic clone was isolated and structurally characterized. The 4F2HC gene spans 8 kilobases of chromosome 11 and is composed of nine exons. The 5' upstream region of the gene displays several properties which are characteristic of housekeeping genes. It is G+C rich and hypomethylated in peripheral blood lymphocyte DNA and contains multiple binding sites for the Sp1 transcription factor while lacking TATA or CCAAT sequences. This region of the gene also displays sequence homologies with several other inducible T-cell genes, including the interleukin-2, interleukin-2 receptor alpha chain, dihydrofolate reductase, thymidine kinase, and transferrin receptor genes. A 255-base-pair fragment of the 4F2HC gene which contains 154 base pairs of the 5' flanking sequence was able to efficiently promote expression of the bacterial chloramphenicol acetyltransferase gene in human Jurkat T cells, indicating that it contains promoter or enhancer (or both) sequences. Analyses of chromatin structure in resting and lectin-activated T cells revealed the presence of stable DNase I-hypersensitive sites within both the 5' flanking and intron 1 regions of the 4F2HC gene. Although the 4F2HC gene displayed many of the structural features characteristic of a constitutively expressed gene, lectin-mediated activation of resting peripheral blood T lymphocytes resulted in a dramatic increase in steady-state levels of 4F2HC mRNA.

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