SOME EFFECTS OF A SINGLE INJECTION OF CHLORMADINONE ACETATE OR METHALLIBURE ON SEXUAL FUNCTION IN THE PUBESCENT MALE ANIMAL

Reproduction ◽  
1969 ◽  
Vol 20 (1) ◽  
pp. 95-104 ◽  
Author(s):  
J. D. SKINNER ◽  
L. E. A. ROWSON
Author(s):  
T. M. Crisp ◽  
F.R. Denys

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.


2006 ◽  
Vol 175 (4S) ◽  
pp. 425-425
Author(s):  
Nasim Zabihi ◽  
Arthur Mourtzinos ◽  
Mary G. Maher ◽  
Shlomo Raz ◽  
Larissa V. Rodriguez

2005 ◽  
Vol 173 (4S) ◽  
pp. 341-341
Author(s):  
Andrea Salonia ◽  
Marina Pontillo ◽  
Fabio Fabbri ◽  
Giuseppe Zanni ◽  
Rita Daverio ◽  
...  

2010 ◽  
Vol 41 (4) ◽  
pp. 2
Author(s):  
DAMIAN McNAMARA
Keyword(s):  

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