scholarly journals Effects of steroids on mouse oocyte maturation in vitro

Reproduction ◽  
1980 ◽  
Vol 60 (2) ◽  
pp. 331-338 ◽  
Author(s):  
D. M. Smith ◽  
D. Y. Tenney
Keyword(s):  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Maturation In Vitro

scholarly journals Perfluorononanoic acid impedes mouse oocyte maturation by inducing mitochondrial dysfunction and oxidative stress

2021 ◽  
Author(s):  
Xiaofei Jiao ◽  
Ning Liu ◽  
Yiding Xu ◽  
Huanyu Qiao
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Oocyte Maturation ◽  
Early Stage ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Toxic Effects ◽  
Germinal Vesicle Breakdown ◽  
Maturation In Vitro

Perfluorononanoic acid (PFNA), a member of PFAS, is frequently detected in human blood and tissues, even in follicular fluid of women. The exposure of PFNA, but not PFOA and PFOS, is positively correlated with miscarriage and increased time to pregnancy. Toxicological studies indicated that PFNA exposure is associated with immunotoxicity, hepatotoxicity, developmental toxicity, and reproductive toxicity in animals. However, there is little information regarding the toxic effects of PFNA on oocyte maturation. In this study, we investigated the toxic effects of PFNA exposure on mouse oocyte maturation in vitro. Our results showed that 600 μM PFNA significantly inhibited germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) in mouse oocytes. Our further study revealed that PFNA induced abnormal metaphase I (MI) spindle assembly, evidenced by malformed spindles and mislocalization of p-ERK1/2 in PFNA-treated oocytes. We also found that PFNA induced abnormal mitochondrial distribution and increased mitochondrial membrane potential. Consequently, PFNA increased reactive oxygen species (ROS) levels, leading to oxidative stress, DNA damage, and eventually early-stage apoptosis in oocytes. In addition, after 14 h culture, PFNA disrupted the formation of metaphase II (MII) spindle in most PFNA-treated oocytes with polar bodies. Collectively, our results indicate that PFNA interferes with oocyte maturation in vitro via disrupting spindle assembly, damaging mitochondrial functions, and inducing oxidative stress, DNA damage, and early-stage apoptosis.

scholarly journals The effect of cold shock on mouse oocyte maturation in vitro

Reproduction ◽  
1978 ◽  
Vol 54 (2) ◽  
pp. 401-403
Author(s):  
D. M. Smith ◽  
D. Y. Tenney
Keyword(s):  
Oocyte Maturation ◽  
Cold Shock ◽  
Mouse Oocyte ◽  
Maturation In Vitro

Forskolin and mouse oocyte maturation in vitro

1984 ◽  
Vol 230 (1) ◽  
pp. 125-129 ◽  
Author(s):  
Eimei Sato ◽  
S. S. Koide
Keyword(s):  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Maturation In Vitro

Effects of hCG, oestradiol and progesterone on mouse oocyte maturation in vitro

1984 ◽  
pp. 83-85
Author(s):  
F. Kayama ◽  
M. Mizuno ◽  
Y. Morita ◽  
K. Sato ◽  
S. Sakamoto
Keyword(s):  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Maturation In Vitro

Effect of FSH on E2/GPR30-mediated mouse oocyte maturation in vitro

2020 ◽  
Vol 66 ◽  
pp. 109464
Author(s):  
Hui Zhao ◽  
Junbang Ge ◽  
Juncai Wei ◽  
Jie Liu ◽  
Chen Liu ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Maturation In Vitro

scholarly journals Effect of hypophysectomy on mouse oocyte maturation in vitro

Reproduction ◽  
1979 ◽  
Vol 55 (2) ◽  
pp. 415-422 ◽  
Author(s):  
D. M. Smith ◽  
D. Y. Tenney
Keyword(s):  
Oocyte Maturation ◽  
Mouse Oocyte ◽  
Maturation In Vitro

scholarly journals Fetuin Inhibits Zona Pellucida Hardening and Conversion of ZP2 to ZPZ during Spontaneous Mouse Oocyte Maturation in Vitro in the Absence of Serum1

1990 ◽  
Vol 43 (5) ◽  
pp. 891-897 ◽  
Author(s):  
Allen C. Schroeder ◽  
Richard M. Schultz ◽  
Gregory S. Kopf ◽  
Frederick R. Taylor ◽  
Robert B. Becker ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Zona Pellucida ◽  
Mouse Oocyte ◽  
Maturation In Vitro
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