scholarly journals Relationship of DNA integrity to HRG C633T SNP and ART outcome in infertile couples

Reproduction ◽  
2017 ◽  
Vol 153 (6) ◽  
pp. 865-876 ◽  
Author(s):  
Arumugam Kumaresan ◽  
Anders Johannisson ◽  
Sarah Nordqvist ◽  
Karin Kårehed ◽  
Helena Åkerud ◽  
...  
Keyword(s):  
Dna Integrity ◽  
Male Factor ◽  
Seminal Parameters ◽  
Sperm Dna Integrity ◽  
Disulphide Bonds ◽  
Sperm Dna ◽  
Significant Difference ◽  
Unknown Factor ◽  
Art Outcome ◽  
Protamine Deficiency

The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome orHRGC633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n = 118) undergoingin vitrofertilisation (IVF) or intracytoplasmic sperm injection (ICSI). Sperm DNA integrity was analysed by flow cytometry using three fluorescent probes (acridine orange, monobromobimane and chromomycin A3). Principal component analysis (PCA) was used to identify the parameters that most influenced fertility. The relationships of sperm DNA integrity with seminal parameters,HRGC633T SNP and ART outcome were established using ANOVA andt-test. Sperm concentration and yield after preparation accounted for 27% of the total variance; sperm DNA integrity (%DFI and disulphide bonds) accounted for 16% of the variance in men from infertile couples. Sperm %DFI was significantly higher (P < 0.05) in older men than in younger men. A significant difference (P < 0.01) was observed in %DFI between smokers and non-smokers. Sperm %DFI was significantly higher (P < 0.01) in male factor infertility compared to either female factor or unknown factor infertility while free thiols were significantly higher (P < 0.01) in unknown infertility factor. No significant difference was observed between IVF success/failure in any of the seminal parameters studied. There was a tendency for protamine deficiency to be higher and disulphide concentration to be lower in men withHRG633T. Such assessments may provide additional useful information about the prognosis for ART outcome, although more research is needed before clinical guidelines can be provided.

scholarly journals DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Abdolrahman Khezri ◽  
Birgitte Narud ◽  
Else-Berit Stenseth ◽  
Anders Johannisson ◽  
Frøydis Deinboll Myromslien ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Fragmentation ◽  
Dna Integrity ◽  
Sperm Cells ◽  
Sperm Dna Integrity ◽  
Disulphide Bonds ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Different Levels ◽  
Protamine Deficiency

Abstract Background Sperm DNA integrity is considered essential for successful transmission of the paternal genome, fertilization and normal embryo development. DNA fragmentation index (DFI, %) has become a key parameter in the swine artificial insemination industry to assess sperm DNA integrity. Recently, in some elite Norwegian Landrace boars (boars with excellent field fertility records), a higher level of sperm DFI has been observed. In order to obtain a better understanding of this, and to study the complexity of sperm DNA integrity, liquid preserved semen samples from elite boars with contrasting DFI levels were examined for protamine deficiency, thiol profile and disulphide bonds. Additionally, the DNA methylation profiles of the samples were determined by reduced representation bisulphite sequencing (RRBS). Results In this study, different traits related to sperm DNA integrity were investigated (n = 18 ejaculates). Upon liquid storage, the levels of total thiols and disulphide bonds decreased significantly, while the DFI and protamine deficiency level increased significantly. The RRBS results revealed similar global patterns of low methylation from semen samples with different levels of DFI (low, medium and high). Differential methylation analyses indicated that the number of differentially methylated cytosines (DMCs) increased in the low-high compared to the low-medium and the medium-high DFI groups. Annotating the DMCs with gene and CpG features revealed clear differences between DFI groups. In addition, the number of annotated transcription starting sites (TSS) and associated pathways in the low-high comparison was greater than the other two groups. Pathway analysis showed that genes (based on the closest TSS to DMCs) corresponding to low-high DFI comparison were associated with important processes such as membrane function, metabolic cascade and antioxidant defence system. Conclusion To our knowledge, this is the first study evaluating DNA methylation in boar sperm cells with different levels of DFI. The present study shows that sperm cells with varying levels of DNA fragmentation exhibit similar global methylation, but different site-specific DNA methylation signatures. Moreover, with increasing DNA fragmentation in spermatozoa, there is an increase in the number of potentially affected downstream genes and their respective regulatory pathways.

scholarly journals PP-002 - Sperm DNA integrity test and ART outcome

2016 ◽  
Vol 32 ◽  
pp. S3
Author(s):  
Violeta Slavcheva Rilcheva ◽  
Nina Pecova Ayvazova ◽  
Lyubomira Ognyanova Ilieva ◽  
Svetlana Ivanova Pachkova ◽  
Emiliana Ilieva Konova
Keyword(s):  
Dna Integrity ◽  
Integrity Test ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Art Outcome

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Renata Simões ◽  
Weber Beringui Feitosa ◽  
Adriano Felipe Perez Siqueira ◽  
Marcilio Nichi ◽  
Fabíola Freitas Paula-Lopes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Cleavage Rate ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Significant Difference

Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

Seminal parameters of chronic male genital inflammation are associated with disturbed sperm DNA integrity

Andrologia ◽  
2015 ◽  
Vol 47 (4) ◽  
pp. 464-469 ◽  
Author(s):  
F. Haidl ◽  
G. Haidl ◽  
I. Oltermann ◽  
J. P. Allam
Keyword(s):  
Dna Integrity ◽  
Seminal Parameters ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Male Genital

scholarly journals Can oocytes repair fragmented DNA of spermatozoa?

2020 ◽  
Vol 8 (2) ◽  
pp. 73-77
Author(s):  
Michal Ješeta ◽  
Markéta Myšková ◽  
Jana Žáková ◽  
Igor Crha ◽  
Karel Crha ◽  
...  
Keyword(s):  
Sperm Cell ◽  
Sperm Count ◽  
External Factors ◽  
Dna Integrity ◽  
Repair System ◽  
Male Factor ◽  
Sperm Dna Integrity ◽  
Fragmented Dna ◽  
Sperm Dna

AbstractApproximately half of the cases of infertility are due to male factor. In many cases the underlying cause of male infertility is not discovered and, therefore, the condition is considered idiopathic. Examination of morphology, motility, concentration and total sperm count is very important but not sufficient for complex men fertility evaluation. Sperm DNA integrity is a very important one. Sperm DNA can be fragmented by several internal or external factors. In immature sperm cells, the DNA can be repaired by reparatory mechanisms of spermatogonia or spermatocytes. However, in a haploid mature sperm cell, these fragments can not be repaired by male and the fragmented DNA is transferred to oocyte. Oocytes are able to repair male fragmented DNA after their fertilization. A quality embryo can repair damaged sperm DNA and the repair system depends on cytoplasmic and genomic quality of the oocyte. The ability of oocyte to repair sperm DNA strong depend on quality of fertilized oocytes.Running title: Oocyte and DNA repair

scholarly journals Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Wiesław Bielas ◽  
Wojciech Niżański ◽  
Agnieszka Partyka ◽  
Anna Rząsa ◽  
Ryszard Mordak
Keyword(s):  
Sperm Parameters ◽  
Dna Integrity ◽  
Long Term Storage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Term Storage
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