Can oocytes repair fragmented DNA of spermatozoa?

AbstractApproximately half of the cases of infertility are due to male factor. In many cases the underlying cause of male infertility is not discovered and, therefore, the condition is considered idiopathic. Examination of morphology, motility, concentration and total sperm count is very important but not sufficient for complex men fertility evaluation. Sperm DNA integrity is a very important one. Sperm DNA can be fragmented by several internal or external factors. In immature sperm cells, the DNA can be repaired by reparatory mechanisms of spermatogonia or spermatocytes. However, in a haploid mature sperm cell, these fragments can not be repaired by male and the fragmented DNA is transferred to oocyte. Oocytes are able to repair male fragmented DNA after their fertilization. A quality embryo can repair damaged sperm DNA and the repair system depends on cytoplasmic and genomic quality of the oocyte. The ability of oocyte to repair sperm DNA strong depend on quality of fertilized oocytes.Running title: Oocyte and DNA repair

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Head‐attached live sperm cell for label‐free micro‐Raman evaluation of sperm DNA integrity: A preliminary study

Journal of Raman Spectroscopy ◽

10.1002/jrs.5811 ◽

2020 ◽

Vol 51 (4) ◽

pp. 591-595

Author(s):

Zufang Huang ◽

ShengRong Du ◽

Xiaozhou Liang ◽

Yan Sun ◽

Xiwen Chen ◽

...

Keyword(s):

Sperm Cell ◽

Dna Integrity ◽

Label Free ◽

Sperm Dna Integrity ◽

Sperm Dna ◽

Preliminary Study ◽

Live Sperm

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Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods

American Journal of Men s Health ◽

10.1177/15579883211001202 ◽

2021 ◽

Vol 15 (2) ◽

pp. 155798832110012

Author(s):

Zhi-Da Shi ◽

Yan-Ping Zhang ◽

Li-Ping Zhai ◽

Mei-Hua Zhang ◽

Yun-Ling Dong ◽

...

Keyword(s):

Western Blotting ◽

Caspase 3 ◽

Abortion Rate ◽

Dna Integrity ◽

Antisperm Antibodies ◽

Cleavage Rate ◽

Sperm Dna Integrity ◽

Sperm Dna ◽

Ivf Et

The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( p = .001 and p < .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( p < 0.05-0.001). There was a higher cleavage rate ( p = .017) and a lower abortion rate ( p < .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART.

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Comparison of microfluidic and swim-up sperm separation methods for IVF

Medical Journal of Cell Biology ◽

10.2478/acb-2020-0022 ◽

2020 ◽

Vol 8 (4) ◽

pp. 170-175

Author(s):

Michal Ješeta ◽

Kateřina Franzová ◽

Jana Žáková ◽

Pavel Ventruba ◽

Igor Crha

Keyword(s):

Microfluidic Chip ◽

Dna Integrity ◽

Sperm Cells ◽

Essential Step ◽

Microfluidic Separation ◽

Sperm Dna Integrity ◽

Fragmented Dna ◽

Sperm Dna ◽

Before And After ◽

Sperm Separation

AbstractSperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been applied more and more frequently in recent years. This method is often presented as extremely gentle to spermatozoa and decreasing significantly concentration of sperm cells with fragmented DNA when compared to conventional methods. The aim of our study was to verify a microfluidic chip system from the perspective of its potential to select spermatozoa with non-fragmented DNA. We tested the efficiency of this separation method against the swim-up method. In this study we evaluated sperm DNA integrity before and after the separation methods in ten patients. Ejaculate of each patient was separated by both the swim up method and the microfluidic chip method at the same time. It was shown that both the methods are very similar in reduction of spermatozoa with fragmented DNA. Interestingly, the concentration of spermatozoa with fragmented DNA was lower after the microfluidic separation than after the swim-up method in all the patients. Nevertheless, the differences were not statistically significant with only 2.1% on average, which is negligible in terms of practical use.Running title: Microfluidic chip and DNA fragmentation

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Relationship of DNA integrity to HRG C633T SNP and ART outcome in infertile couples

Reproduction ◽

10.1530/rep-17-0058 ◽

2017 ◽

Vol 153 (6) ◽

pp. 865-876 ◽

Cited By ~ 1

Author(s):

Arumugam Kumaresan ◽

Anders Johannisson ◽

Sarah Nordqvist ◽

Karin Kårehed ◽

Helena Åkerud ◽

...

Keyword(s):

Dna Integrity ◽

Male Factor ◽

Seminal Parameters ◽

Sperm Dna Integrity ◽

Disulphide Bonds ◽

Sperm Dna ◽

Significant Difference ◽

Unknown Factor ◽

Art Outcome ◽

Protamine Deficiency

The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome orHRGC633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n = 118) undergoingin vitrofertilisation (IVF) or intracytoplasmic sperm injection (ICSI). Sperm DNA integrity was analysed by flow cytometry using three fluorescent probes (acridine orange, monobromobimane and chromomycin A3). Principal component analysis (PCA) was used to identify the parameters that most influenced fertility. The relationships of sperm DNA integrity with seminal parameters,HRGC633T SNP and ART outcome were established using ANOVA andt-test. Sperm concentration and yield after preparation accounted for 27% of the total variance; sperm DNA integrity (%DFI and disulphide bonds) accounted for 16% of the variance in men from infertile couples. Sperm %DFI was significantly higher (P < 0.05) in older men than in younger men. A significant difference (P < 0.01) was observed in %DFI between smokers and non-smokers. Sperm %DFI was significantly higher (P < 0.01) in male factor infertility compared to either female factor or unknown factor infertility while free thiols were significantly higher (P < 0.01) in unknown infertility factor. No significant difference was observed between IVF success/failure in any of the seminal parameters studied. There was a tendency for protamine deficiency to be higher and disulphide concentration to be lower in men withHRG633T. Such assessments may provide additional useful information about the prognosis for ART outcome, although more research is needed before clinical guidelines can be provided.

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