Time-resolved fluorescence polarization spectroscopy and optical imaging of smart receptor-targeted contrast agents in tissues for cancer detection

2013 ◽  
pp. 368-392 ◽  
Author(s):  
Y. Pu ◽  
R. Alfano
Keyword(s):  
Contrast Agents ◽  
Optical Imaging ◽  
Cancer Detection ◽  
Fluorescence Polarization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Polarization Spectroscopy ◽  
Targeted Contrast Agents

scholarly journals Fluorescence anisotropy measurements in solution: Methods and reference materials (IUPAC Technical Report)

2013 ◽  
Vol 85 (3) ◽  
pp. 589-608 ◽  
Author(s):  
Marcel Ameloot ◽  
Martin vandeVen ◽  
A. Ulises Acuña ◽  
Bernard Valeur
Keyword(s):  
Steady State ◽  
Fluorescence Anisotropy ◽  
Fluorescence Polarization ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Steady State Fluorescence ◽  
Technical Report ◽  
Accuracy Of Measurements ◽  
Solution Methods ◽  
Systematic Effects

After recalling the basic relations relevant to both steady-state and time-resolved fluorescence polarization, it is shown how the values of steady-state polarized intensities recorded experimentally usually need to be corrected for systematic effects and errors, caused by instrumentation and sample properties. A list of selected reference values of steady-state fluorescence anisotropy and polarization is given. Attention is also paid to analysis of time-resolved fluorescence anisotropy data obtained by pulse fluorometry or phase and modulation fluorometry techniques. Recommendations for checking the accuracy of measurements are provided together with a list of selected time-resolved fluorescence anisotropy data as reported in the literature.

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