After recalling the basic relations relevant to both steady-state and
time-resolved fluorescence polarization, it is shown how the values of
steady-state polarized intensities recorded experimentally usually need to be
corrected for systematic effects and errors, caused by instrumentation and
sample properties. A list of selected reference values of steady-state
fluorescence anisotropy and polarization is given. Attention is also paid to
analysis of time-resolved fluorescence anisotropy data obtained by pulse
fluorometry or phase and modulation fluorometry techniques. Recommendations for
checking the accuracy of measurements are provided together with a list of
selected time-resolved fluorescence anisotropy data as reported in the
literature.