An Efficient Method of Genomic DNA Isolation from Mucilage-Rich Okra Leaves for Molecular biology studies

AbstrakProtokol dan metode sederhana, efisien untuk isolasi DNA genom tanaman durian yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan protokol isolasi DNA tumbuhan dengan metode CTAB yang dimodifikasi sebagai protokol yang efisien untuk membuang polisakarida, phenol dan lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini terdiri dari protocol CTAB yang dimodifikasi tahap inkubasi dan presipitasi pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2 protokol isolasi DNA durian dengan CTAB standard an CTAB modifikasi menunjukkan bahwa metode CTAB modifikasi menghasilkan whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi dengan PCR-RAPD.Kata kunci: isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB at the incubate and precipitate procedure employing DNA purification step to remove polysaccharides and phenol residu. Compared with the two studied DNA isolation protocols of durian using standart CTAB and modified CTAB the everage yield high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA, polysaccharides, phenol residu, CTAB

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Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v4i8.700-705.788 ◽

2016 ◽

Vol 4 (8) ◽

pp. 700 ◽

Cited By ~ 1

Author(s):

Muhammad Amjad Nawaz ◽

Faheem Shehzad Baloch ◽

Hafiz Mamoon Rehman ◽

Bao Le ◽

Fahima Akther ◽

...

Keyword(s):

Molecular Biology ◽

Dna Extraction ◽

Genomic Dna ◽

Dna Isolation ◽

Plant Molecular Biology ◽

Pcr Amplification ◽

Cost Effective ◽

Extraction Methods ◽

Glycine Species ◽

Genomic Dna Isolation

Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

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An efficient method of genomic DNA isolation from plant tissues.

Acta Biochimica Polonica ◽

10.18388/abp.1995_4629 ◽

1995 ◽

Vol 42 (3) ◽

pp. 329-331 ◽

Cited By ~ 1

Author(s):

P M Strózycki ◽

A B Legocki

Keyword(s):

Efficient Method ◽

Genomic Dna ◽

Dna Isolation ◽

Lupinus Luteus ◽

Plant Tissues ◽

Easy Method ◽

Genomic Dna Isolation

The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.

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