scholarly journals Pengembangan Protokol Isolasi DNA Genom Tanaman Durian Dengan Menggunakan Modifikasi Bufer CTAB

2018 ◽  
Vol 6 (02) ◽  
pp. 33
Author(s):  
Sundari Sundari
Keyword(s):  
Efficient Method ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Dna Purification ◽  
Fruit Crops ◽  
Purification Step ◽  
High Quality ◽  
Plant Dna ◽  
Genomic Dna Isolation

AbstrakProtokol dan metode sederhana, efisien untuk isolasi  DNA genom tanaman durian   yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan  protokol isolasi DNA tumbuhan  dengan metode CTAB yang dimodifikasi sebagai protokol yang  efisien untuk membuang polisakarida, phenol  dan  lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini  terdiri dari protocol CTAB yang dimodifikasi  tahap inkubasi dan presipitasi  pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2  protokol  isolasi  DNA durian dengan CTAB standard an CTAB modifikasi  menunjukkan bahwa metode  CTAB modifikasi menghasilkan  whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi  dengan PCR-RAPD.Kata kunci:  isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient  method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB   at the incubate  and precipitate procedure employing DNA purification  step to remove polysaccharides and phenol residu.  Compared with the two  studied DNA  isolation protocols of  durian using standart CTAB and modified CTAB  the everage yield  high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA,  polysaccharides,  phenol residu, CTAB

Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae)

2012 ◽  
Vol 18 (1) ◽  
pp. 40-45
Author(s):  
Elena Servienė ◽  
Irena Kemežienė ◽  
Jūratė Kasperovičienė ◽  
Brigita Čapukoitienė ◽  
Vida Rančelienė ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variability ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Rapd Analysis ◽  
Dna Purification ◽  
High Quality ◽  
Purification Method ◽  
Rapd Pcr ◽  
Gonyostomum Semen

Abstract Servienė E., Kemežienė I., Kasperovičienė J., čapukoitienė B., Rančelienė V., Koreivienė J., 2012: Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae) [DNR izoliavimas ir PGR parametrų optimizavimas Gonyostomum semen (Raphidophyceae) dumblių RAPD analizei]. - Bot. Lith., 18(1): 40-45. The genomic DNA purification method for Gonyostomum semen algae was optimized by applying different DNA purification techniques and rational modifications. This method allowed to obtain high quality DNA preparations suitable for the phylogenetic analysis and genetic variability investigation of algae. DNA isolated by this method yielded strong and reliable amplification products showing their applicability for RAPD-PCR using random decamer primers. In the present study, the RAPD protocol was optimized for the evaluation of Gonyostomum biodiversity.

scholarly journals A one-tube method for rapid and reliable plant genomic DNA isolation for PCR analysis

2020 ◽  
Author(s):  
Wei Hu ◽  
J. Clark Lagarias
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Low Cost ◽  
Plant Molecular Biology ◽  
Pcr Analysis ◽  
High Quality ◽  
Plant Dna ◽  
Genomic Dna Isolation ◽  
Dna Extraction Method

AbstractBackgroundConsistent isolation of high quality plant genomic DNA is a prerequisite for successful PCR analysis. Time consumption, ease of operation and procedure cost are important secondary considerations for selecting an effective DNA extraction method. The simple, reliable and rapid DNA extraction method developed by Edwards and colleagues in 1991 [1] has proven to be the gold standard.ResultsThrough modification of the Edwards method of extraction, we have developed a one-tube protocol that greatly improves the efficiency of plant DNA extraction and reduces the potential for sample contamination while simultaneously yielding high quality DNA suitable for PCR analysis. We further show that DNA extracts prepared with this method are stable at room temperature for at least three months.ConclusionThe one-tube extraction method yields high quality plant DNA with improved efficiency while greatly minimizing the potential for cross contamination. This low-cost and environment-friendly method is widely applicable for plant molecular biology research.

scholarly journals Genomic DNA isolation from scrophularieae dried leaves using a simple, high-throughput protocol

2019 ◽  
Vol 48 (4) ◽  
pp. 1231-1235
Author(s):  
Mehrshid Riahi ◽  
Melina Babaei ◽  
Farrokh Ghahremaninejad
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Extraction ◽  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
High Molecular Weight Dna ◽  
Plant Dna ◽  
Genomic Dna Isolation

This communication described efficient DNA extraction from Scrophularia and Verbascum samples. Modified Murray and Thompson modified Cota-Sànchez method and Bioflux kit methods were applied for the extraction of DNA. Among the different methods, Bioflux kit Plant DNA extraction kit, coupled with some modification was the best for extraction of high molecular weight DNA as long as the extracted DNA is quantified with fluorescence-based methods.

scholarly journals An efficient method of genomic DNA isolation from plant tissues.

1995 ◽  
Vol 42 (3) ◽  
pp. 329-331 ◽  
Author(s):  
P M Strózycki ◽  
A B Legocki
Keyword(s):  
Efficient Method ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Lupinus Luteus ◽  
Plant Tissues ◽  
Easy Method ◽  
Genomic Dna Isolation

The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.

scholarly journals An alternative method for Staphylococcus aureus DNA isolation

2008 ◽  
Vol 60 (2) ◽  
pp. 299-306 ◽  
Author(s):  
L. Chapaval ◽  
D.H. Moon ◽  
J.E. Gomes ◽  
F.R. Duarte ◽  
S.M. Tsai
Keyword(s):  
Staphylococcus Aureus ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Environmental Isolates ◽  
Reaction Volume ◽  
High Quality ◽  
Rapid Procedure ◽  
Final Reaction ◽  
Staphylococcal Enterotoxins ◽  
Final Reaction Volume

This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.

Genomic DNA Isolation, Amplification of the D1S80 Locus and DNA-Gel Electrophoresis

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane
Keyword(s):  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation ◽  
D1s80 Locus
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