scholarly journals A novel strain of Aspergillus niger producing a cocktail of hydrolytic depolymerising enzymes for the production of second generation biofuels

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 552-569 ◽  
Author(s):  
Namita Bansal ◽  
Rupinder Tewari ◽  
Jugal Kishore Gupta ◽  
Raman Soni ◽  
Sanjeev Kumar Soni
Keyword(s):  
Aspergillus Niger ◽  
Second Generation ◽  
Hydrolytic Enzymes ◽  
Reducing Sugar ◽  
Soil Samples ◽  
Domestic Waste ◽  
Second Generation Biofuels ◽  
Pectinolytic Enzymes ◽  
Enzyme Cocktail ◽  
Conversion Efficiencies

The screening and isolation of fungi producing a cocktail of hydrolytic enzymes was studied. Among the various isolates obtained from different soil samples, a strain NS-2 was selected. The phylogenetic analysis of this strain showed highest homology (99%) with Aspergillus niger. It was capable of producing cellulolytic, hemicellulolytic, amylolytic, and pectinolytic enzymes in appreciable titers on wheat bran based liquid and solid state media. The mixture of enzymes produced by this organism could effectively hydrolyze various domestic waste residues, revealing conversion efficiencies of 89 to 92% and produced high reducing sugar yields of 0.48 to 0.66 g/g of dry residue. This enzyme cocktail could potentially find a significant application in the conversion of agricultural and other waste residues having cellulose, hemicellulose, starch, and pectin as carbohydrates to produce simpler sugars which can be fermented for the production of second generation biofuels.

scholarly journals Cocktail of carbohydrases from Aspergillus niger: an economical and eco-friendly option for biofilm clearance from biopolymer surfaces

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arashdeep Kaur ◽  
Sanjeev Kumar Soni ◽  
Shania Vij ◽  
Praveen Rishi
Keyword(s):  
Aspergillus Niger ◽  
Laser Scanning ◽  
Statistical Modelling ◽  
Laser Scanning Microscopy ◽  
Enzyme Treatment ◽  
Confocal Laser ◽  
Abiotic Surfaces ◽  
Enzyme Cocktail ◽  
Natural Variant

AbstractBiofilm formation on both biotic and abiotic surfaces accounts for a major factor in spread of antimicrobial resistance. Due to their ubiquitous nature, biofilms are of great concern for environment as well as human health. In the present study, an integrated process for the co-production of a cocktail of carbohydrases from a natural variant of Aspergillus niger was designed. The enzyme cocktail was found to have a noteworthy potential to eradicate/disperse the biofilms of selected pathogens. For application of enzymes as an antibiofilm agent, the enzyme productivities were enhanced by statistical modelling using response surface methodology (RSM). The antibiofilm potential of the enzyme cocktail was studied in terms of (i) in vitro cell dispersal assay (ii) release of reducing sugars from the biofilm polysaccharides (iii) the effect of enzyme treatment on biofilm cells and architecture by confocal laser scanning microscopy (CLSM). Potential of the enzyme cocktail to disrupt/disperse the biofilm of selected pathogens from biopolymer surfaces was also assessed by field emission scanning electron microscopy (FESEM) analysis. Further, their usage in conjunction with antibiotics was assessed and it was inferred from the results that the use of enzyme cocktail augmented the efficacy of the antibiotics. The study thus provides promising insights into the prospect of using multiple carbohydrases for management of heterogeneous biofilms formed in natural and clinical settings.

Boosting the Guerbet Reaction: a cooperative catalytic system for the efficient bio-ethanol refinery to second-generation biofuels

2021 ◽  
Author(s):  
Cristiana Cesari ◽  
Anna Gagliardi ◽  
Alessandro Messori ◽  
Nicola Monti ◽  
Valerio Zanotti ◽  
...  
Keyword(s):  
Catalytic System ◽  
Second Generation ◽  
Second Generation Biofuels ◽  
Guerbet Reaction

Optimal design of supply chains for second generation biofuels incorporating European biofuel regulations

2016 ◽  
Vol 133 ◽  
pp. 565-575 ◽  
Author(s):  
Laura Elisabeth Hombach ◽  
Claudia Cambero ◽  
Taraneh Sowlati ◽  
Grit Walther
Keyword(s):  
Optimal Design ◽  
Supply Chains ◽  
Second Generation ◽  
Second Generation Biofuels

Integration of Ethanol Fermentation with Second Generation Biofuels Technologies

Biorefineries ◽  
2014 ◽  
pp. 161-187 ◽  
Author(s):  
Caroline Häggström ◽  
Ulrika Rova ◽  
Tomas Brandberg ◽  
David B. Hodge
Keyword(s):  
Ethanol Fermentation ◽  
Second Generation ◽  
Second Generation Biofuels

Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation

2018 ◽  
Vol 37 (2) ◽  
pp. 149-156 ◽  
Author(s):  
C. Marzo ◽  
A.B. Díaz ◽  
I. Caro ◽  
A. Blandino
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Hydrolytic Enzymes ◽  
Industrial Wastes ◽  
Added Value ◽  
Orange Peels ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of ORA were 31,000 U·kg-1 and 17,600 U·kg-1, respectively; while for ESBC the maximum values reached were 35,000 U·kg-1 and 28,000 U·kg-1, respectively. The enzymatic extracts obtained in the SSF experiments were also employed for the hydrolysis of ORA and ESBC. Furthermore, it was found that extracts obtained from SSF of ORA, supplemented with commercial cellulase, were more efficient for the hydrolysis of ORA and ESBC than a commercial enzyme cocktail typically used for this purpose. In this case, maximum reducing sugars concentrations of 57 and 47 g·L-1 were measured after the enzymatic hydrolysis of ESBC and ORA, respectively.

Research and Production of Second-Generation Biofuels

2019 ◽  
pp. 383-400
Author(s):  
H.L. Raghavendra ◽  
Shashank Mishra ◽  
Shivaleela P. Upashe ◽  
Juliana F. Floriano
Keyword(s):  
Second Generation ◽  
Second Generation Biofuels
Sign in / Sign up

Export Citation Format

Share Document