scholarly journals Usefulness of Rat Skin as a Substitute for Human Skin in the in Vitro Skin Permeation Study

2011 ◽  
Vol 60 (4) ◽  
pp. 373-384 ◽  
Author(s):  
Hiroyuki TAKEUCHI ◽  
Yoko MANO ◽  
Shuichi TERASAKA ◽  
Takanobu SAKURAI ◽  
Atsushi FURUYA ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Permeation ◽  
Rat Skin ◽  
Permeation Study ◽  
In Vitro Skin Permeation

scholarly journals Elastic liposomes mediated transdermal delivery of verapamil hydrochloride

2018 ◽  
Vol 8 (6) ◽  
pp. 16-21
Author(s):  
NEHA JAIN ◽  
Ameeta Argal ◽  
Girendra Gautam
Keyword(s):  
Transdermal Delivery ◽  
Skin Permeation ◽  
Entrapment Efficiency ◽  
Sprague Dawley ◽  
Rat Skin ◽  
Verapamil Hydrochloride ◽  
In Vitro Skin Permeation ◽  
Permeation Studies ◽  
Elastic Vesicles

The aim of present investigation was to formulate and characterize elastic liposomes as a delivery system for transdermal delivery of Verapamil hydrochloride, a drug having low oral bioavailability (approx 20%), short biological half-life and extensive first pass metabolism.Verapamil hydrochloride loaded elastic vesicles were prepared by a slightly modified extrusion method using soya phosphatidylcholine and span 80(edge activator). Prepared elastic vesicles were characterized for various parameters such as vesicle shape, vesicle size and size distribution, entrapment efficiency, elasticity measurements, stability studies and in vitro skin permeation studies through excised rat skin (Sprague Dawley) using a locally fabricated Franz diffusion cell. The entrapment efficiency of elastic vesicles was found to be 59.3±3.6%. In vitro skin permeation of verapamil hydrochloride through excised rat skin (Sprague Dawley) revealed that elastic vesicles led to an enhanced transdermal flux (50.2±4.52 mg/cm2/h) of verapamil hydrochloride as compared to liposomes (11.6±2.12mg/cm2/h). Decreased lag time (0.9 h) was also observed in case of elastic liposomes. Our results indicate the feasibility of elastic liposomes for transdermal delivery of verapamil hydrochloride for improved skin permeation. Keywords: Transdermal delivery, Elastic liposomes, Verapamil hydrochloride.  

Transdermal Delivery of Quercetin Using Elastic Liposomes: Preparation, Characterization and In Vitro Skin Permeation Study

Polymer Korea ◽  
2012 ◽  
Vol 36 (6) ◽  
pp. 705-711 ◽  
Author(s):  
Soo Nam Park ◽  
Myoung Sun Lim ◽  
Min A Park ◽  
Soon Sik Kwon ◽  
Seat Byeol Han
Keyword(s):  
Transdermal Delivery ◽  
Skin Permeation ◽  
Permeation Study ◽  
In Vitro Skin Permeation

Transdermal Delivery of Metoprolol II: In-Vitro Skin Permeation and Bioavailability in Hairless Rats

1995 ◽  
Vol 84 (2) ◽  
pp. 158-160 ◽  
Author(s):  
Tapash K. Ghosh ◽  
Joseph Adir ◽  
Si‐Ling Xiang ◽  
Samuel Onyilofur
Keyword(s):  
Transdermal Delivery ◽  
Skin Permeation ◽  
In Vitro Skin Permeation

Skin permeation of retinol in Tween 20-based deformable liposomes: in-vitro evaluation in human skin and keratinocyte models

2006 ◽  
Vol 58 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Yu-Kyoung Oh ◽  
Mi Young Kim ◽  
Jee-Young Shin ◽  
Tae Woon Kim ◽  
Mi-Ok Yun ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Permeation ◽  
Tween 20 ◽  
In Vitro Evaluation

scholarly journals Penetration of Chlorhexidine into Human Skin

2008 ◽  
Vol 52 (10) ◽  
pp. 3633-3636 ◽  
Author(s):  
T. J. Karpanen ◽  
T. Worthington ◽  
B. R. Conway ◽  
A. C. Hilton ◽  
T. S. J. Elliott ◽  
...  
Keyword(s):  
Human Skin ◽  
High Performance ◽  
Skin Permeation ◽  
Full Thickness ◽  
Alternative Strategies ◽  
Thickness Skin ◽  
Skin Antisepsis ◽  
Permeation Studies ◽  
Franz Diffusion Cells

ABSTRACT This study evaluated a model of skin permeation to determine the depth of delivery of chlorhexidine into full-thickness excised human skin following topical application of 2% (wt/vol) aqueous chlorhexidine digluconate. Skin permeation studies were performed on full-thickness human skin using Franz diffusion cells with exposure to chlorhexidine for 2 min, 30 min, and 24 h. The concentration of chlorhexidine extracted from skin sections was determined to a depth of 1,500 μm following serial sectioning of the skin using a microtome and analysis by high-performance liquid chromatography. Poor penetration of chlorhexidine into skin following 2-min and 30-min exposures to chlorhexidine was observed (0.157 ± 0.047 and 0.077 ± 0.015 μg/mg tissue within the top 100 μm), and levels of chlorhexidine were minimal at deeper skin depths (less than 0.002 μg/mg tissue below 300 μm). After 24 h of exposure, there was more chlorhexidine within the upper 100-μm sections (7.88 ± 1.37 μg/mg tissue); however, the levels remained low (less than 1 μg/mg tissue) at depths below 300 μm. There was no detectable penetration through the full-thickness skin. The model presented in this study can be used to assess the permeation of antiseptic agents through various layers of skin in vitro. Aqueous chlorhexidine demonstrated poor permeation into the deeper layers of the skin, which may restrict the efficacy of skin antisepsis with this agent. This study lays the foundation for further research in adopting alternative strategies for enhanced skin antisepsis in clinical practice.

In vitro skin permeation of buprenorphine transdermal patch

2020 ◽  
Vol 16 (6) ◽  
Author(s):  
C.-L. Liao ◽  
C.-C. Huang ◽  
C.-Y. Lee ◽  
T.-H. Chiu ◽  
S.-C. Kuo ◽  
...  
Keyword(s):  
Skin Permeation ◽  
Transdermal Patch ◽  
In Vitro Skin Permeation
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