Managing Unintentional Iatrogenic Triple Lumen Central Venous Catheter (TLCVC) Arterial Puncture

Unintentional arterial puncture by central venous catheter insertion/placing can result in destructive complications especially if a large bore (>7F) catheter was used. Unplanned immediate catheter ejection with manual external compression is hazardous due to potential torrential blood leakage or formation of a pseudoaneurysm. Endovascular removal with a vascular closure device deployment is preferred in this situation. The aim of this case report is to discuss the alternative strategies if the vascular closure device fails to secure hemostasis.

Tracking mutational semantics of SARS-CoV-2 genomes

Genomes have an inherent context dictated by the order in which the nucleotides and higher order genomic elements are arranged in the DNA/RNA. Learning this context is a daunting task, governed by the combinatorial complexity of interactions possible between ordered elements of genomes. Can natural language processing be employed on these orderly, complex and also evolving datatypes (genomic sequences) to reveal the latent patterns or context of genomic elements (e.g Mutations)? Here we present an approach to understand the mutational landscape of Covid-19 by treating the temporally changing (continuously mutating) SARS-CoV-2 genomes as documents. We demonstrate how the analogous interpretation of evolving genomes to temporal literature corpora provides an opportunity to use dynamic topic modeling (DTM) and temporal Word2Vec models to delineate mutation signatures corresponding to different Variants-of-Concerns and tracking the semantic drift of Mutations-of-Concern (MoC). We identified and studied characteristic mutations affiliated to Covid-infection severity and tracked their relationship with MoCs. Our ground work on utility of such temporal NLP models in genomics could supplement ongoing efforts in not only understanding the Covid pandemic but also provide alternative strategies in studying dynamic phenomenon in biological sciences through data science (especially NLP, AI/ML).

Untranslatable wounds

A good starting point for revisiting the intersections of language, gender and sexuality is to acknowledge and understand how colonial wounds and legacies play out in our everyday lives. This essay critically addresses the multiple ways in which we are all marked in one way or another by our colonial relations and their intersections. A careful unpacking of mechanisms and linkages is critical for identifying strategies and tactics of struggle that might lead to more equitable present-days characterised by esperanza (hope). Yet a desire to decolonise language and language practices without recognising the lived experience of our own messy and colonial entanglements will never be enough to resignify the systems that hold racial, ethnic, gender, sexual and linguistic inequalities in place. This essay highlights the acts of desbordar (undoing/overflowing), trasto-car (queering) and resentir (feeling again) as alternative strategies that can be used to fracture the architectures of colonialism, starting with our own.

STRATEGI PENGEMBANGAN MANGGIS RATU TEMBILAHAN DI KECAMATAN TEMBILAHAN HULU

Mangosteen is fruit commodity that was sold in the form of fresh fruit and could be processed as herbal medicine and cosmetic. Market demand of mangosteen increase both of in the country and export market. Sub district of Tembilahan have variety of mangosteen that superior quality it’s name mangosteen of Ratu Tembilahan. The aims of this study to (1) know potential development of mangosteen in Sub district of Tembilahan Hulu, (2) determine strategies of mangosteen development in Sub district of Tembilahan Hulu. The data analysis was done using the Location Quotient (LQ) dan SWOT analysis. The results of the study were (1) Mangosteen was superior commodity in Sub district of Tembilahan Hulu (LQ= 1.76), (2) alternative strategies for mangosteen develepment were (a) replanting for old mangosteen, (b) Sertification of mother trees for sources of seed that used by local farmers, (c) improve management of mangosteen farming, (d) training of mangosteen processing to be derivative products. Manggis merupakan komoditas buah yang dikonsumsi sebagai buah segar maupun diolah menjadi obat herbal dan kosmetik. Permintaan manggis mengalami peningkatan baik untuk pasar dalam negeri maupun pasar ekspor. Kecamatan Tembilahan Hulu memiliki varietas manggis yang unggul bernama manggis Ratu Tembilahan. Tujuan penelitian ini adalah (1) untuk mengetahui potensi pengembangan manggis Ratu Tembilahan; (2) menentukan strategi pengembangan manggis Ratu Tembilahan. Metode analisis data menggunakan analisis LQ dan analisis SWOT. Hasil penelitian menunjukkan bahwa (1) Manggis merupakan komoditas basis di Kecamatan Tembilahan Hulu dengan nilai LQ= 1,76, (2) alternatif strategi pengembangan manggis Tembilahan Hulu adalah (a) Peremajaan tanaman manggis tua, (b) Sertifikasi pohon induk sebagai sumber bibit para penangkar bibit lokal, (c) Peningkatan pengelolaan usaha tani manggis, (d) Pelatihan pembuatan produk olahan manggis.

Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci.

Background: The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i.e.: two loxP sites flanking an exon or a critical DNA sequence of interest) remain the most challenging to generate as they require simultaneous cleavage of the genome using two guides in order to properly integrate the repair template. In the current manuscript, we describe a modification of the sequential electroporation procedure described by Horii et al (2017). We demonstrate production of conditional allele mouse models for eight different genes via one of two alternative strategies: either by consecutive sequential electroporation (strategy A) or non-consecutive sequential electroporation (strategy B). Results: By using strategy A, we demonstrated successful generation of conditional allele models for three different genes (Icam1, Lox, and Sar1b), with targeting efficiencies varying between 5 to 13%. By using strategy B, we generated five conditional allele models (Loxl1, Pard6a, Pard6g, Clcf1, and Mapkapk5), with targeting efficiencies varying between 3 to 25%. Conclusion: Our modified electroporation-based approach, involving one of the two alternative strategies, allowed the production of conditional allele models for eight different genes via two different possible paths. This reproducible method will serve as another reliable approach in addition to other well-established methodologies in the literature for conditional allele mouse model generation.

Neuromodulatory Approaches for Atrial Fibrillation Ablation

In this review, the authors describe evolving alternative strategies for the management of AF, focusing on non-invasive and percutaneous autonomic modulation. This modulation can be achieved – among other approaches – via tragus stimulation, renal denervation, cardiac afferent denervation, alcohol injection in the vein of Marshall, baroreceptor activation therapy and endocardial ganglionated plexi ablation. Although promising, these therapies are currently under investigation but could play a role in the treatment of AF in combination with conventional pulmonary vein isolation in the near future.

PEMBERDAYAAN STRATEGI UMKM MELALUI PENGABDIAN KEPADA MASYARAKAT DENGAN PENDEKATAN ANALISIS SWOT

ABSTRAKUMKM Farida Snack dan Aphe berupaya menjadi produsen kerupuk kulit yang memiliki nilai lebih di mata konsumenya. Mengingat saat ini masih berlangsungnya ketidakstabilan ekonomi yang disebabkan oleh pandemi covid-19. Sehingga membuat para pelaku UMKM perlu melakukan analisis strategi untuk menjaga produksi yang tetap berjalan. Melalui Pengabdian Kepada Masyarakat ini dengan tujuan untuk pemberdayaan strategi UMKM. Analisis yang dilakukan dengan pendekatan analisis SWOT, dengan lokasi pengabdian kepada masyarakat di Kabupaten Karawang. Hasil dari pengabdian ini adalah diperolehnya analisis SWOT dan rekomendasi yang dapat diimplementasikan. Maka melalui Matriks SWOT yang diperoleh berdasarkan hasil S-O (Strength-Opportunities), W-O (Weaknesses-Opportunities), S-T (Strength-Threat) dan W-T (Weaknesses-Threat). Sehingga melalui Matriks SWOT bertujuan untuk mencocokkan peluang dan ancaman sebagai faktor eksternal yang dihadapi perusahaan dengan kekuatan dan kelemahan sebagai internalnya untuk menghasilkan strategi alternatif. Analisis tersebut menggunakan suatu matriks yang terdiri dari 9 sel yang berisikan 4 sel alternatif strategi yang dikatakan sebagai Matriks SWOT. Kata kunci: covid-19 pandemic; SWOT analysis; SWOT matrix. ABSTRACTFarida Snack and Aphe strives to be a producer of skin crackers that have more value in the eyes of consumers. Considering the current economic instability caused by the covid-19 pandemic. So that makes MSMEs need to do strategy analysis to keep production running. Through this Community Service with the aim of empowering MSMEs strategies. The analysis is carried out with a SWOT analysis approach, with the location of community service in Karawang Regency. The result of this devotion is the acquisition of SWOT analysis and recommendations that can be implemented. Thus through the SWOT Matrix obtained based on the results of S-O (Strength-Opportunities), W-O (Weaknesses-Opportunities), S-T (Strength-Threat) and W-T (Weaknesses-Threat). Thus through the SWOT Matrix aims to match opportunities and threats as external factors facing the company with strengths and weaknesses as its internal to produce alternative strategies. The analysis uses a matrix consisting of 9 cells containing 4 alternative cell strategies that are said to be swot matrix.. Keywords: covid-19 pandemic; SWOT analysis; SWOT matrix.

A Comparison of Vulnerability Risks and Conservation Perceptions between Mariculture, Fishery and Ecotourism Livelihood Groups in a Multi-Use MPA in Indonesia

Different livelihoods have different vulnerability risks and influences on the management of marine protected areas (MPAs). This research aimed to compare the seasonal, trend and shock livelihood vulnerability indicators (LVIs) of three dominant livelihood groups and the groups’ perceptions towards supporting MPA conservation efforts. The Anambas Archipelago MPA was selected as the study site. A total of 66 respondents from the three major groups were selected using stratified random sampling and interviewed using a questionnaire containing 14 LVIs. The responses were standardised and aggregated using functional relationships. The groups’ perceptions were determined using frequency distribution and thematic analyses (NVIVO 10). The LVI composite values showed that fishers were the most vulnerable (0.65), followed by fish farmers (0.62) and ecotourism operators (0.47). Fishers and fish farmers expressed high vulnerability due to their dependency on the coastal resources. The ecotourism operators had low vulnerability due to their lower dependency on natural resources, smaller impacts from seasonal weather, low involvement in resource conflicts and greater political support. The three groups supported the MPA regimes despite differences in their knowledge of the MPA restrictions on their livelihood practices. The study’s findings provide key alternative strategies to address the vulnerability risks of the three major groups and to increase their support for conservation goals in similar MPAs.