Phytochemical screening &evaluation of ant diarrheal mode of action of leaves extracts of Paederia foetida linn

ABSTRAKTanaman sembukan (Paederia foetida) atau yang lebih kita kenal dengan daun kentut merupakan tanaman yang biasanya digunakan oleh masyarakat sebagai obat diare atau obat kembung. Kandungan senyawa metabolit sekunder tanaman sembukan perlu dikaji lebih lanjut untuk penemuan bahan obat baru. Tujuan dari penelitian adalah untuk menganalisis kandungan senyawa metabolit sekunder yang terdapat pada daun dan batang sembukan dengan metode maserasi yang direndam ke dalam pelarut etanol 96% dan metanol selama 5 hari. Ekstrak etanol dan ekstrak metanol pada masing-masing daun dan batang kemudian dianalisis dengan menggunakan reagen kimia untuk mengidentifikasi senyawa alkaloid, saponin, tanin dan flavonoid. Hasil skrining fitokimia menunjukkan bahwa pada ekstrak etanol dan metanol batang sembukan mengandung alkaloid, saponin, tanin dan flavonoid. Sedangkan pada ekstrak etanol dan metanol daun sembukan mengandung alkaloid, tanin dan flavonoid. Selanjutnya senyawa metabolit sekunder tersebut dianalisis aktivitas biologisnya.Kata kunci: Analisis senyawa metabolit sekunder, ekstrak etanol dan metanol daun dan batang sembukan (Paederia foetida), maserasiABSTRACTPaederia foetida or more familiar with fart leaves is a plant that is usually used by the community as a drug or bloated diarrhea. The content of the secondary metabolite compound of the Paederia foetida plant needs to be studied further for the discovery of new drug ingredients. The purpose of this research is to analyze the content of secondary metabolite compounds found on leaves and stems with a maseration method soaked in 96% ethanol solvent and methanol for 5 days. The ethanol extract and methanol extract on each leaf and stem were then analyzed using chemical reagents to identify the alkaloid compounds, saponins, tanins and flavonoids. The results of phytochemical screening showed that in the ethanol extract and methanol the stem of the Paederia foetida contained alkaloids, saponins, tanins and flavonoids. While on ethanol extract and methanol leaves Paederia foetida contains alkaloids, tanins and flavonoids. Furthermore, the secondary metabolite compounds are analyzed biological activity.Keywords: Analysis of secondary metabolite compounds, ethanol extract and methanol leaves and stirring stem (Paederia foetida), maceration

Download Full-text

Phytochemical Screening, Antibacterial Activity, and Mode of Action on Morus nigra

Pharmacognosy Journal ◽

10.5530/pj.2018.1.28 ◽

2017 ◽

Vol 10 (1) ◽

pp. 167-171 ◽

Cited By ~ 3

Author(s):

Diah Lia Aulifa ◽

Sani Nurlaela Fitriansyah ◽

Seno Aulia Ardiansyah ◽

Diki Prayugo Wibowo ◽

Yolanda Armiliani Julata ◽

...

Keyword(s):

Antibacterial Activity ◽

Mode Of Action ◽

Phytochemical Screening ◽

Morus Nigra

Download Full-text

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066917 ◽

1971 ◽

Vol 29 ◽

pp. 570-571

Author(s):

E. A. Elfont ◽

R. B. Tobin ◽

D. G. Colton ◽

M. A. Mehlman

Keyword(s):

Chemical Composition ◽

Rat Liver ◽

Future Study ◽

Mode Of Action ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Effective Inhibitor ◽

Biochemical Effects ◽

Glycerophosphate Dehydrogenase ◽

Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Download Full-text