scholarly journals Development and Validation of Spectrophotometric Methods Manipulating Ratio Spectra for Determination of Tetramisole Hydrochloride in the Presence of its Alkali-Induced Degradation Product

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Ahmed H Abdel-monem ◽  
Mohammed WI Nassar ◽  
Khalid AM Attia ◽  
Ahmad A Mohamad ◽  
Ragab AM Said
Keyword(s):  
Degradation Product ◽  
Spectrophotometric Methods ◽  
Development And Validation

DC Polarographic and Spectrophotometric Determination of Ampicillin Capsules

1980 ◽  
Vol 63 (5) ◽  
pp. 1049-1051
Author(s):  
Juan A Squella ◽  
Luis J Nunez-Vergara ◽  
Maximo Aros
Keyword(s):  
Absorption Band ◽  
Spectrophotometric Determination ◽  
Degradation Product ◽  
Uv Absorption ◽  
Acidic Hydrolysis ◽  
Average Recovery ◽  
Polarographic Wave ◽  
Spectrophotometric Methods ◽  
Hydrolysis Of

Abstract Polarographic and spectrophotometric methods are proposed for the determination of ampicillin in capsules. Acidic hydrolysis of ampicillin with 1% HCHO in 0.3N HCl yields a degradation product identified as 2-hydroxy-3-phenyl-6-methylpyrazine. This compound has a well defined UV absorption band at 380 nm and a polarographic wave at –0.55 V vs SCE, which can be used for analytical purposes. Individual capsule assays, composite assays, and recovery studies are described. The average recovery values and standard deviations (SD) for UV and polarographic determinations were 99.20% (SD 0.95) and 100.85% (SD 1.09), respectively

scholarly journals Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Shereen Mowaka ◽  
Bassam M. Ayoub ◽  
Mostafa A. Hassan ◽  
Wafaa A. Zaghary
Keyword(s):  
Degradation Product ◽  
Simultaneous Equation ◽  
Chemometric Methods ◽  
Acid Degradation ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Mean Centering ◽  
Validation Parameters ◽  
Major Acid

New spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid degradation product (TAD) and applied successfully as a stability indicating assay to recently approved Zafatek® tablets. TAD was monitored using TLC to ensure complete degradation. Furthermore, HPLC was used to confirm dealing with one major acid degradation product. The proposed methods were developed by manipulating zero-order, first-derivative, and ratio spectra of TRG and TAD using simultaneous equation, first-derivative, and mean-centering methods, respectively. Using Spectra Manager II and Minitab v.14 software, the absorbance at 274 nm–260.4 nm, amplitudes at 260.4 nm–274.0 nm, and mean-centered values at 287.6 nm–257.2 nm were measured against methanol as a blank for TRG and TAD, respectively. Linearity and the other validation parameters were acceptable at concentration ranges of 5–50 μg/mL and 2.5–25 μg/mL for TRG and TAD, respectively. Using one-way analysis of variance (ANOVA), the optimized methods were compared and proved to be accurate for the simultaneous assay of TRG and TAD.

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