ASSESSMENT OF GENETIC STRUCTURE VARIABILITY OF RAINBOW TROUT Oncorhynchus mykiss (WALBAUM, 1792) OF UKRAINIAN LOCAL STOCKS USING POLYMORPHIC BLOOD PLASMA PROTEINS

Rainbow trout is a valuable species of aquaculture, which is characterized by a high level of variability. Aim. The goal was to study the peculiarities of genetic variability of rainbow trout based on polymorphism of transferrin (TF), posttransferrin (PTF), esterase (EST) (EC 3.1.1.1.) and albumin (ALB) loci. Methods. Electrophoretic separation of plasma proteins of rainbow trout from three local stocks (Chernivtsi, Kharkiv and Transcarpathian) was carried out in the native polyacrylamide gel. Results. Peculiarities of the distribution and relative electrophoretic mobility of allelic variants of the studied loci of rainbow trout of Ukrainian local stocks were established.The results demonstrated the effectiveness of the analysis using methods of biochemical genetics to establish the characteristics of the genetic structure, the level of heterozygosity of local stocks and to carry out their differentiation. Conclusion. The use of selected markers would allow monitoring the dynamics of changes in the state of local stocks under the established conditions of cultivation in the future. Key words: rainbow trout, polymorphic blood plasma proteins, transferrin, posttransferrin, esterase, albumin.

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Gossypol isomers bind specifically to blood plasma proteins and spermatozoa of rainbow trout fed diets containing cottonseed meal

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/s0304-4165(00)00168-9 ◽

2001 ◽

Vol 1525 (1-2) ◽

pp. 37-42 ◽

Cited By ~ 37

Author(s):

Konrad Dabrowski ◽

Kyeong-Jun Lee ◽

Jacques Rinchard ◽

Andrzej Ciereszko ◽

Joost H Blom ◽

...

Keyword(s):

Rainbow Trout ◽

Blood Plasma ◽

Cottonseed Meal ◽

Plasma Proteins ◽

Blood Plasma Proteins

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Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

PROTEOMICS ◽

10.1002/pmic.201600460 ◽

2017 ◽

Vol 17 (11) ◽

pp. 1600460 ◽

Cited By ~ 6

Author(s):

Joanna Nynca ◽

Georg Arnold ◽

Thomas Fröhlich ◽

Andrzej Ciereszko

Keyword(s):

Rainbow Trout ◽

Blood Plasma ◽

Seminal Plasma ◽

Plasma Proteins ◽

Seminal Plasma Proteins ◽

Blood Plasma Proteins ◽

Proteomic Identification

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Proteomic identification of rainbow trout seminal plasma proteins and their relationship to blood plasma proteins

Reproductive Biology ◽

10.1016/j.repbio.2013.01.130 ◽

2013 ◽

Vol 13 ◽

pp. 7

Author(s):

J. Nynca ◽

G. Arnold ◽

T. Fröhlich ◽

K. Otte ◽

F. Flenkenthaler ◽

...

Keyword(s):

Rainbow Trout ◽

Blood Plasma ◽

Seminal Plasma ◽

Plasma Proteins ◽

Seminal Plasma Proteins ◽

Blood Plasma Proteins ◽

Proteomic Identification

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Genetic structure of rainbow trout Oncorhynchus mykiss (Salmoniformes, Salmonidae) from aquaculture by DNA-markers

Biosystems Diversity ◽

10.15421/012104 ◽

2021 ◽

Vol 29 (1) ◽

pp. 28-32

Author(s):

О. Y. Bielikova ◽

A. E. Mariutsa ◽

A. I. Mruk ◽

S. I. Tarasjuk ◽

V. M. Romanenko

Keyword(s):

Rainbow Trout ◽

Genetic Structure ◽

Dna Markers ◽

Shannon Index ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Expected Heterozygosity ◽

High Level ◽

Issr Pcr ◽

Local Stock ◽

Simple Sequence

The rational use of valuable fish species from aquaculture is difficult to implement without knowledge of the state of the genetic structure of local stocks. Different types of DNA markers can be used to achieve the goals of selection and breeding work. The genetic structure of a local stock of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) (Salmoniformes, Salmonidae) farmed in Ukraine was studied using DNA-markers: microsatellite (SSR-markers – simple-sequence repeats-markers) and intermicrosatellite (ISSR – inter-simple sequence repeat). Five fragments of trinucleotide microsatellite motifs with a single anchor nucleotide at the 3'-end were used as a primer for analysis by the ISSR-PCR method. Totally, 85 amplicons were obtained across the five loci, of which 92.9% were polymorphic. The total number of alleles ranged from 10 (marker (ACC)₆G) to 23 (marker (AGC)₆G). The following monomorphic amplicons were determined for the studied local stock of rainbow trout: according to marker (CTC)₆C – 770 and 520 bp bands, for the marker (GAG)₆C – 345, 295 and 260 bp, and for the marker (AGC)₆C – 350 bp. The average number of polymorphic bands per locus was 15.8. The selected ISSR primers had a level of polymorphic information content above the average. The most effective markers for molecular-genetic analysis of rainbow trout were (AGC)₆G and (AGC)₆C according to the percentage of polymorphic bands, marker index, effective multiplex ratio and resolving power. The selected ISSR loci allow the genetic structure of the studied local stock to be characterized using the total and the effective number of alleles per locus (Na and Ne were 1.9 and 1.4, respectively), the Shannon index (average value I was 0.4) and the unbiased expected heterozygosity (mean uHe = 0.3). Microsatellite-based analysis showed features of the genetic structure of the local stock of rainbow trout at six microsatellite loci (OMM 1032, OMM 1077, OMM 1088, Str 15, Str 60, Str 73). Allelic diversity was established and alleles with the highest frequency and most typical for the given stock were identified. The Shannon index and unbiased expected heterozygosity were determined using SSR-markers and were 1.42 and 0.79, respectively. This depicts the complexity of the population structure, a high level of genetic diversity and indicates a high level of heterozygosity of local stock. The “gene pool profile” established as a result of ISSR-PCR in the future will help to differentiate local stocks of rainbow trout in aquaculture of Ukraine. Microsatellite markers provide the ability to determine individual features of genetic variation of local populations and to conduct the management of genetic resources on fish farms.

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