Screening Test for the Diagnosis of Chronic Granulomatous Disease

Normal phagocytes suspended in plasma when mixed with latex particles for ingestion and nitroblue tetrazolium dye will reduce the dye to a blue color which may be easily seen in a test tube. This reaction depends on the release of enzymes from phagocytic lysosomal granules after phagocytosis. Since such release is deficient in patients with CGD, no blue color develops in the tube. This has proved to be a simple, reliable, screening test which should allow the more rapid and widespread diagnosis of CGD.

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The Nylon Column Dye Test: A Possible Screening Test of Phagocyte Function

Clinical Science ◽

10.1042/cs0490591 ◽

1975 ◽

Vol 49 (6) ◽

pp. 591-596

Author(s):

A. W. Segal ◽

T. J. Peters

Keyword(s):

Chronic Granulomatous Disease ◽

Whole Blood ◽

Screening Test ◽

Test Procedure ◽

Nitroblue Tetrazolium ◽

Screening Procedure ◽

Granulomatous Disease ◽

Quantitative Test ◽

Dye Reduction ◽

Blue Coloration

1. A simple quantitative test has been developed to investigate phagocyte function. 2. This test is performed by the addition of Nitroblue Tetrazolium to whole blood, followed by the isolation of leucocytes on a column of nylon wool. Dye reduction by phagocytes is apparent as a blue coloration of the column due to the formation of formazan. The formazan can be extracted from the column and measured spectrophotometrically. 3. The formation of formazan was found to be directly related to the number of phagocytes in blood. 4. Two patients with chronic granulomatous disease gave abnormal results, suggesting that the test procedure may be of value as a screening procedure for this disease.

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Decreased Nitroblue Tetrazolium Dye Reduction in the Phagocytes of Patients Receiving Prednisone

PEDIATRICS ◽

10.1542/peds.45.5.861 ◽

1970 ◽

Vol 45 (5) ◽

pp. 861-865

Author(s):

Denis R. Miller ◽

Henry G. Kaplan

Keyword(s):

Chronic Granulomatous Disease ◽

Nitroblue Tetrazolium ◽

Quantitative Assay ◽

Granulomatous Disease ◽

Host Defenses ◽

Dye Reduction ◽

Slide Test ◽

Intracellular Metabolism

Nitroblue tetrazolium (NBT) dye reduction by leukocytes of 21 patients receiving prednisone was significantly decreased. Nineteen percent of the patients had values similar to those found in children with chronic granulomatous disease, and 57% had heterozygous-range NBT dye reduction. A qualitative NBT dye reduction "slide test" correlated well with the quantitative assay. The uptake of particles by the phagocytes of steroid-treated patients appeared normal. The exact mechanism of corticosteroid action remains unknown. The decreased dye reduction observed in vitro suggests an induced defect of intracellular metabolism which may be related to known alterations of host defenses which occur in patients receiving these hormones.

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A Study on the Nitroblue Tetrazolium (NBT) Reduction Test of Cells Migrating to the Tissue: The NBT Test of Cells Migrating to the Tissue in Healthy Adults and Patients with Chronic Granulomatous Disease (CGD)

Pediatrics International ◽

10.1111/j.1442-200x.1976.tb01476.x ◽

1976 ◽

Vol 18 (1) ◽

pp. 45-46

Author(s):

Kosuke Joh

Keyword(s):

Chronic Granulomatous Disease ◽

Healthy Adults ◽

Nitroblue Tetrazolium ◽

Granulomatous Disease ◽

Reduction Test ◽

Nbt Test ◽

Nbt Reduction

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A variant form of X-linked chronic granulomatous disease with normal nitroblue tetrazolium slide test and cytochrome b

European Journal of Clinical Investigation ◽

10.1111/j.1365-2362.1983.tb00095.x ◽

1983 ◽

Vol 13 (3) ◽

pp. 243-247 ◽

Cited By ~ 26

Author(s):

NIELS BORREGAARD ◽

ANDREW R. CROSS ◽

TROELS HERLIN ◽

OWEN T. G. JONES ◽

ANTHONY W. SEGAL ◽

...

Keyword(s):

Chronic Granulomatous Disease ◽

Cytochrome B ◽

Nitroblue Tetrazolium ◽

Variant Form ◽

Granulomatous Disease ◽

Slide Test

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FIBROBLAST NITROBLUE TETRAZOLIUM TEST AND THE IN-UTERO DIAGNOSIS OF CHRONIC GRANULOMATOUS DISEASE

The Lancet ◽

10.1016/s0140-6736(80)90554-1 ◽

1980 ◽

Vol 315 (8158) ◽

pp. 18-19 ◽

Cited By ~ 16

Author(s):

SenihM. Fikrig ◽

Kamala Suntharalingam ◽

ElizabethM. Smithwick ◽

RobertA. Good

Keyword(s):

Chronic Granulomatous Disease ◽

In Utero ◽

Nitroblue Tetrazolium ◽

Granulomatous Disease ◽

Nitroblue Tetrazolium Test ◽

Tetrazolium Test

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The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

Blood ◽

10.1182/blood.v48.2.309.bloodjournal482309 ◽

1976 ◽

Vol 48 (2) ◽

pp. 309-313 ◽

Cited By ~ 2

Author(s):

RL Baehner ◽

LA Boxer ◽

J Davis

Keyword(s):

Chronic Granulomatous Disease ◽

Polymorphonuclear Leukocytes ◽

Nitroblue Tetrazolium ◽

Granulomatous Disease ◽

Biochemical Basis ◽

Nitroblue Tetrazolium Reduction ◽

Normal Human ◽

Nbt Reduction ◽

Human Polymorphonuclear Leukocytes ◽

Tetrazolium Reduction

Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1–14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

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