Neonatal Necrotizing Enterocolitis Associated with Penicillin-Resistant, Toxigenic Clostridium butyricum

PEDIATRICS ◽  
1980 ◽  
Vol 66 (6) ◽  
pp. 928-931 ◽  
Author(s):  
Randall Sturm ◽  
Joseph L. Staneck ◽  
Larry R. Stauffer ◽  
Wallace W. Neblett
Keyword(s):  
Cerebrospinal Fluid ◽  
Necrotizing Enterocolitis ◽  
Peritoneal Fluid ◽  
Pseudomembranous Colitis ◽  
Toxin Production ◽  
Clostridium Butyricum ◽  
Intestinal Diseases ◽  
Clostridial Infection ◽  
Neonatal Necrotizing Enterocolitis

The cause of neonatal necrotizing enterocolitis (NEC) is unknown. An association between NEC and clostridial infection has been reported from several centers, but the organisms have not been extensively characterized. Clostridium butyricum was isolated from the peritoneal fluid and cerebrospinal fluid of a neonate with NEC. The organism was resistant to the penicillins, but sensitive to vancomycin. Toxin production was demonstrated. Although the role of clostridial toxins in the pathogenesis of NEC is unknown, clostridial toxins are well established as the causes of two other intestinal diseases (antibiotic-associated pseudomembranous colitis and pig-bel). Further investigation of the role of clostridia in the pathogenesis of NEC and of the use of oral, nonabsorbable antibiotics in the treatment of NEC is needed.

Intestinal Lesions Containing Coronavirus-like Particles in Neonatal Necrotizing Enterocolitis: An Ultrastructural Analysis

PEDIATRICS ◽  
1984 ◽  
Vol 73 (2) ◽  
pp. 218-224
Author(s):  
S. Rousset ◽  
O. Moscovici ◽  
P. Lebon ◽  
J. P. Barbet ◽  
P. Helardot ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Small Intestine ◽  
Necrotizing Enterocolitis ◽  
Intestinal Mucosa ◽  
Light And Electron Microscopy ◽  
Anaerobic Bacteria ◽  
Intestinal Lesions ◽  
Maternity Hospitals ◽  
Neonatal Necrotizing Enterocolitis

Since the outbreaks of neonatal necrotizing enterocolitis occurring in maternity hospitals of Paris and suburbs in 1979-1980, it has been possible to examine by light and electron microscopy gut specimens from ten newborns with this illness. Coronavirus-like particles, enclosed in intracytoplasmic vesicles of damaged epithelial cells of the intestinal mucosa, were observed in the small intestine, appendix, and colon. The ultrastructural study, supported by bacteriologic findings, suggests the role of coronavirus-like particles in the appearance of the lesions. Secondary proliferation of mainly anaerobic bacteria, probably responsible for pneumatosis, may aggravate the disease.

scholarly journals Epidemiological and genetic characterization of Clostridium butyricum cultured from neonatal cases of necrotizing enterocolitis in China

2020 ◽  
Vol 41 (8) ◽  
pp. 900-907
Author(s):  
Yinping Dong ◽  
Ying Li ◽  
Di Zhang ◽  
Scott Nguyen ◽  
Nikunj Maheshwari ◽  
...  
Keyword(s):  
Necrotizing Enterocolitis ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Genetic Characterization ◽  
Pulsed Field Gel Electrophoresis ◽  
Species Level ◽  
Clostridium Butyricum ◽  
Neonatal Necrotizing Enterocolitis ◽  
Dna Profiles

AbstractObjective:Laboratory-based characterization and traceback of Clostridium butyricum isolates linked to outbreak cases of neonatal necrotizing enterocolitis (NEC) in a hospital in China.Methods:In total, 37 samples were collected during the NEC outbreak. Classical bacteriological methods were applied to isolate and identify Clostridium spp. Meanwhile, 24 samples collected after an outbreak were similarly tested. All Clostridium isolates were identified to species level as either C. butyricum or C. sporogenes. These isolates were subsequently subtyped using pulsed-field gel electrophoresis (PFGE). Genomic DNA was purified from 2 representative C. butyricum isolates and sequenced to completion.Results:Of 37 samples collected during the NEC outbreak, 17 (45.95%) were positive for Clostridium spp. One species, C. butyricum, was cultured from 10 samples. Another species cultured from 2 other samples was identified as C. sporogenes. Both of these species were cocultured from 5 samples. Pulsotyping showed that the 15 C. butyricum and the 7 C. sporogenes isolates produced indistinguishable DNA profiles. No NEC cases were reported after disinfection following the outbreak, and all samples collected after the outbreak were negative for Clostridium spp. Whole-genome sequencing (WGS) indicated that sialidase, hemolysin, and enterotoxin virulence factors were located on the chromosomes of 2 C. butyricum isolates.Conclusions:The outbreak of NEC was epidemiologically linked to C. butyricum contamination within the hospital. This is the first report of an NEC outbreak associated with C. butyricum infection in China.

Role of Clostridium Perfringens in Neonatal Necrotizing Enterocolitis and the Utility of Anaerobic Blood Cultures

2021 ◽  
pp. 1-4
Author(s):  
Alejandra Sandoval C ◽  
◽  
María Paz Romero O ◽  
Carolina Guerra E ◽  
Carolina Riquelme B ◽  
...  
Keyword(s):  
Necrotizing Enterocolitis ◽  
Clostridium Perfringens ◽  
Diagnostic Method ◽  
Blood Cultures ◽  
Clinical Suspicion ◽  
High Complexity ◽  
Gram Positive ◽  
Neonatal Necrotizing Enterocolitis

Introduction: Clostridium perfringens is a gram-positive anaerobic bacillus that causes various clinical diseases, including necrotizing enterocolitis in newborns. The progression could be fulminant and lethal. Presentation of the cases: We report 3 cases of necrotizing enterocolitis due to C. perfringens from 2 high-complexity public centers in Chile. Conclusion: Strong clinical suspicion combined with early microbiological identification may modify patient evolution. Anaerobic blood cultures have become a fundamental diagnostic method; therefore, having this tool in neonatal units would be useful

Neonatal Necrotizing Enterocolitis Associated with Penicillin-Resistant, Toxigenic Clostridium butyricum

PEDIATRICS ◽  
1981 ◽  
Vol 67 (4) ◽  
pp. 569-569
Author(s):  
Randall Sturm ◽  
Joseph L. Staneck ◽  
Larry R. Stauffer ◽  
Wallace W. Neblett
Keyword(s):  
Newborn Infant ◽  
Necrotizing Enterocolitis ◽  
Clostridium Butyricum ◽  
Neonatal Necrotizing Enterocolitis

In the article "Neonatal Necrotizing Enterocolitis Associated with Penicillin Resistant, Toxigenic Clostridium butyricum" by Sturm et al (Pediatrics 66: 928-931, 1980) reference 2 (p930) should read as follows: Burrington JD: Necrotizing enterocolitis in the newborn infant. Clin Perinatol 5:29, 1978.

Clostridium difficile Infection: A Model for Disruption of the Gut Microbiota Equilibrium

2016 ◽  
Vol 34 (3) ◽  
pp. 217-220 ◽  
Author(s):  
Julie Blanchi ◽  
Julien Goret ◽  
Francis Mégraud
Keyword(s):  
Human Physiology ◽  
Gastrointestinal Tract ◽  
Clostridium Difficile ◽  
Gut Microbiota ◽  
Pseudomembranous Colitis ◽  
Toxin Production ◽  
Endogenous Factors ◽  
Complex Ecosystem ◽  
Composition Changes

The gut microbiota is a complex ecosystem defined by the combination of microorganisms living in the gastrointestinal tract. Its equilibrium is intimately involved in several aspects of vital process for human physiology and nutrition. Its composition changes depending on both exogenous and endogenous factors. The disruption of the gut microbiota by antibiotics often leads to an opportunistic infection by Clostridium difficile. The unbalanced intestinal microbiota promotes spore germination, growth of vegetative forms and toxin production leading to C. difficile infection, which is characterized by diarrhea and possibly pseudomembranous colitis. This nosocomial infection is a good model to understand the role of the gut microbiota in preventing the development of pathogens.

The role of clostridia as pathogens in neonatal necrotizing enterocolitis

1980 ◽  
Vol 96 (4) ◽  
pp. 787-788
Author(s):  
M.F. Smith ◽  
G.S. Clayden ◽  
M.W. Casewell ◽  
S.P. Borriello
Keyword(s):  
Necrotizing Enterocolitis ◽  
Neonatal Necrotizing Enterocolitis
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