EFFECTS OF CULTURE DENSITY ON GROWTH AND IN VITRO TUBERIZATION CAPACITY OF POTATO PLANTLETS

2000 ◽  
Vol 48 (2) ◽  
pp. 185-189
Author(s):  
K. Tábori ◽  
A. Ferenczy ◽  
J. Dobránszki
Keyword(s):  
Solanum Tuberosum ◽  
Plant Density ◽  
Solanum Tuberosum L ◽  
Stem Length ◽  
Tuber Initiation ◽  
In Vitro Tuberization ◽  
In Vitro Plantlets ◽  
Plant Densities ◽  
Potato Plantlets

In vitro plantlets of four varieties of potato (Solanum tuberosum L.) were grown at different plant densities to study their effects on growth and microtuber yield. The responses of the cultivars to plant density, as expressed by changes in development characteristics, especially stem length and leaf area, were different. The time of tuber initiation was earlier at higher plantlet densities for all cultivars. The microtuber number per plantlet was not affected by the plantlet density. The highest number of well-sized (≯4 mm) microtubers per jar could be harvested from the higher plantlet densities (30–40 plantlets per jar). In addition to good yield, the microtubers were uniform in these treatments, so they appear to be economical.

scholarly journals The Effects of Different Concentrations of Gibberellic Acid (GA3) and Kinetin on in Vitro Tuberization of Potato (Solanum tuberosum L.)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 752C-752
Author(s):  
Servet Kefi ◽  
Paul E. Read ◽  
Alexander Pavlista ◽  
Stephen D. Kachman
Keyword(s):  
Solanum Tuberosum ◽  
Gibberellic Acid ◽  
Solanum Tuberosum L ◽  
Short Photoperiod ◽  
In Vitro Tuberization ◽  
High Concentration ◽  
In Vitro Plantlets ◽  
Murashige And Skoog Medium ◽  
Cultivar Atlantic

To determine the influence of gibberellic acid (GA3) and 6-furfuryl aminopurine (kinetin) concentrations alone and in combinations on in vitro tuberization of potato, nine treatments consisting of combinations of gibberellic acid and kinetin at three levels of concentration (0, 2, and 5 mg·liter–1) were included in Murashige and Skoog medium supplemented with 6% sucrose. Four single nodes of in vitro plantlets from Solanum tuberosum L. cultivar Atlantic were placed into each magenta box. All magenta boxes were arranged in a randomized complete box design with five replications and cultured under a short photoperiod condition (8 h light/16 h dark). Gibberellic acid strongly inhibited tuberization when used alone or with kinetin, whereas kinetin induced tuberization at both 2 and 5 mg·liter–1. Although tuberization was initiated in the absence of kinetin because of the high concentration of sucrose and short photoperiod, the presence of kinetin accelerated the in vitro tuberization process of potato.

Effect of photoperiod on in vitro tuberization of potato (Solanum tuberosum L.)

1993 ◽  
Vol 34 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Janet E. A. Seabrook ◽  
Shirlyn Coleman ◽  
David Levy
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization

scholarly journals In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress

2017 ◽  
Vol 3 (5) ◽  
pp. 188
Author(s):  
Bilter Anton Sirait ◽  
Rosa Charloq
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum ◽  
Drought Stress ◽  
Banding Pattern ◽  
Solanum Tuberosum L ◽  
In Vitro Study ◽  
Dry Weight ◽  
Leaf Chlorophyll ◽  
Potato Plantlets

<p class="Els-Abstract-text"><em>In vitro</em> preliminary studies is candidate tolerant of potato (<em>Solanum tuberosum</em> L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to <em>in vitro</em> drought stress conditions using Polyethylene Glycol PEG.  This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg · L<sup>–</sup><sup>1</sup>, P2 = 6 000 mg · L<sup>–</sup><sup>1</sup>.  The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but  the increase in  the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09  in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.</p>

Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions

2001 ◽  
Vol 44 (4) ◽  
pp. 315-325 ◽  
Author(s):  
K. Pruski ◽  
P. Duplessis ◽  
T. Lewis ◽  
T. Astatkie ◽  
J. Nowak ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization ◽  
Dark Conditions

scholarly journals EFFECT OF SILVER THIOSULPHATE AND PHOTOPERIOD ON IN VITRO TUBERIZATION OF THREE POTATO (SOLANUM TUBEROSUM L.) CULTIVARS

2021 ◽  
Vol 21 (Suppliment-1) ◽  
pp. 308-317
Author(s):  
S.F. El-Sayed ◽  
Sahar S. Taha ◽  
Omaima S. Darwish ◽  
Saumu Z. Mwessongo
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
In Vitro Tuberization ◽  
Silver Thiosulphate

scholarly journals Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L.

2020 ◽  
Vol 69 (3) ◽  
pp. 357-365
Author(s):  
GUSTAVO YAÑEZ-OCAMPO ◽  
MARTHA E. MORA-HERRERA ◽  
ARNOLDO WONG-VILLARREAL ◽  
DENISSE M. DE LA PAZ-OSORIO ◽  
NADIA DE LA PORTILLA-LÓPEZ ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
Liquid Medium ◽  
Plant Growth Promotion ◽  
Bacterial Strain ◽  
Bacillus Pumilus ◽  
Growth Promotion ◽  
Solanum Tuberosum L ◽  
Stem Length

The capacity of four bacterial strains isolated from productive soil potato fields to solubilize tricalcium phosphate on Pikovskaya agar or in a liquid medium was evaluated. A bacterial strain was selected to evaluate in vitro capacity of plant-growth promotion on Solanum tuberosum L. culture. Bacterial strain A3 showed the highest value of phosphate solubilization, reaching a 20 mm-diameter halo and a concentration of 350 mg/l on agar and in a liquid medium, respectively. Bacterial strain A3 was identified by 16S rDNA analysis as Bacillus pumilus with 98% identity; therefore, it is the first report for Bacillus pumilus as phosphate solubilizer. Plant-growth promotion assayed by in vitro culture of potato microplants showed that the addition of bacterial strain A3 increased root and stems length after 28 days. It significantly increased stem length by 79.3%, and duplicated the fresh weight of control microplants. In this paper, results reported regarding phosphorus solubilization and growth promotion under in vitro conditions represent a step forward in the use of innocuous bacterial strain biofertilizer on potato field cultures.

Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

2017 ◽  
Author(s):  
Yunita Prameswari ◽  
FNU Djenal ◽  
FNU Damanhuri
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

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