Greenhouse production of potato (Solanum tuberosum L. cv. Désirée) seed tubers using in vitro plantlets and rooted cuttings in large propagation beds

1995 ◽  
Vol 38 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Regan D. Roy ◽  
V. Souza Machado ◽  
S. M. M. Alam ◽  
A. Ali
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Greenhouse Production ◽  
Rooted Cuttings ◽  
Seed Tubers ◽  
In Vitro Plantlets

EFFECTS OF CULTURE DENSITY ON GROWTH AND IN VITRO TUBERIZATION CAPACITY OF POTATO PLANTLETS

2000 ◽  
Vol 48 (2) ◽  
pp. 185-189
Author(s):  
K. Tábori ◽  
A. Ferenczy ◽  
J. Dobránszki
Keyword(s):  
Solanum Tuberosum ◽  
Plant Density ◽  
Solanum Tuberosum L ◽  
Stem Length ◽  
Tuber Initiation ◽  
In Vitro Tuberization ◽  
In Vitro Plantlets ◽  
Plant Densities ◽  
Potato Plantlets

In vitro plantlets of four varieties of potato (Solanum tuberosum L.) were grown at different plant densities to study their effects on growth and microtuber yield. The responses of the cultivars to plant density, as expressed by changes in development characteristics, especially stem length and leaf area, were different. The time of tuber initiation was earlier at higher plantlet densities for all cultivars. The microtuber number per plantlet was not affected by the plantlet density. The highest number of well-sized (≯4 mm) microtubers per jar could be harvested from the higher plantlet densities (30–40 plantlets per jar). In addition to good yield, the microtubers were uniform in these treatments, so they appear to be economical.

scholarly journals The Effects of Different Concentrations of Gibberellic Acid (GA3) and Kinetin on in Vitro Tuberization of Potato (Solanum tuberosum L.)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 752C-752
Author(s):  
Servet Kefi ◽  
Paul E. Read ◽  
Alexander Pavlista ◽  
Stephen D. Kachman
Keyword(s):  
Solanum Tuberosum ◽  
Gibberellic Acid ◽  
Solanum Tuberosum L ◽  
Short Photoperiod ◽  
In Vitro Tuberization ◽  
High Concentration ◽  
In Vitro Plantlets ◽  
Murashige And Skoog Medium ◽  
Cultivar Atlantic

To determine the influence of gibberellic acid (GA3) and 6-furfuryl aminopurine (kinetin) concentrations alone and in combinations on in vitro tuberization of potato, nine treatments consisting of combinations of gibberellic acid and kinetin at three levels of concentration (0, 2, and 5 mg·liter–1) were included in Murashige and Skoog medium supplemented with 6% sucrose. Four single nodes of in vitro plantlets from Solanum tuberosum L. cultivar Atlantic were placed into each magenta box. All magenta boxes were arranged in a randomized complete box design with five replications and cultured under a short photoperiod condition (8 h light/16 h dark). Gibberellic acid strongly inhibited tuberization when used alone or with kinetin, whereas kinetin induced tuberization at both 2 and 5 mg·liter–1. Although tuberization was initiated in the absence of kinetin because of the high concentration of sucrose and short photoperiod, the presence of kinetin accelerated the in vitro tuberization process of potato.

Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

2017 ◽  
Author(s):  
Yunita Prameswari ◽  
FNU Djenal ◽  
FNU Damanhuri
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

Оценка влияния отдельных факторов на производство безвирусных мини-клубней картофеля

2021 ◽  
pp. 3-7
Author(s):  
Елена Васильевна Шищенко ◽  
Елена Николаевна Барсукова
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L

Одним из этапов технологического процесса производства оригинального семенного картофеля является выращивание оздоровленных пробирочных микрорастений в весенне-летней теплице для получения мини-клубней. Исследования проводили в Приморском крае на оздоровленных безвирусных пробирочных растениях картофеля (Solanum tuberosum L.) сорта Метеор, размноженных методом микрочеренкования в культуре in vitro. Данный сорт был выбран вследствие его скороспелости. В ходе эксперимента при выращивании микрорастений изучено влияние сочетания факторов — объем пробирок, состав почвенного грунта и площадь питания растения на производство мини-клубней картофеля в условиях искусственного освещения. Дана оценка продуктивности пробирочных растений и определена роль основного фактора, влияющего на выход мини-клубней картофеля сорта Метеор, в лабораторных условиях. Установлено, что основное влияние на производство стандартных мини-клубней картофеля оказал состав почвенного грунта. Максимальное количество стандартных мини-клубней картофеля сорта Метеор (209,3 и 156,1 шт./м2) получено в вариантах опыта при использовании грунта с содержанием элементов питания: фосфора — 1380 мг/кг, калия — 1425 мг/кг, азота — 1394 мг/кг, органического вещества — 66,7 %.

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