Evaluation of inbred lines as sources of new alleles for improving elite maize hybrid

2006 ◽  
Vol 34 (2-3) ◽  
pp. 941-948 ◽  
Author(s):  
Tomislav Zivanovic ◽  
Savo Vuckovic ◽  
Slaven Prodanovic ◽  
Goran Todorovic
Keyword(s):  
Inbred Lines ◽  
Maize Hybrid ◽  
New Alleles

scholarly journals Keragaman Genetik Inbrida Jagung QPM dan Normal Berbasis Marka Mikrosatelit dan Hubungannya dengan Penampilan Hibrida

2016 ◽  
Vol 4 (2) ◽  
pp. 77
Author(s):  
Marcia B. Pabendon ◽  
M. Azrai ◽  
M. J. Mejaya ◽  
Sutrisno Sutrisno
Keyword(s):  
Grain Yield ◽  
Field Trials ◽  
Inbred Lines ◽  
Heterotic Group ◽  
Maize Hybrid ◽  
Normal Maize ◽  
Single Cross ◽  
Upgma Cluster Analysis ◽  
Hybrid Program ◽  
And Performance

<p>Information on genetic divergence of<br />inbred lines and performance of the hybrids developed from<br />the lines is a great value in maize hybrid program. A study<br />was conducted to evaluate genetic diversity of six QPM and<br />five normal maize inbred lines, to determine the relationship<br />between genetic distance based on SSR markers and the<br />grain yield of single cross hybrid, and to get information<br />promising hybrid from the single cross of QPM hybrid.<br />Twenty four polymorphic primers that covered the 10 maize<br />chromosomes were used to fingerprint the lines, detecting<br />in 94 alleles (average of 3.9 and a range of 2-6 alleles per<br />locus). Genetic divergences were determined using the<br />Jaccard’s similarity coefficient, and a dendrogram was<br />constructed using the UPGMA. Cluster analysis divided the<br />inbreds into two clusters that were confirmed by principal<br />coordinate analysis. Two promising QPM hybrids that are<br />crossed from different heterotic group were found. The<br />estimated value of simple correlations (r) of GDs with the<br />gain yield of single cross hybrid was negatif (-0.07). There is<br />a need to conduct more field trials to obtain more accurate<br />correlations, particularly in a practical utility for predicting<br />maize hybrid performance for grain yield.</p>

scholarly journals SNP- Based Assessment of Genetic Purity and Diversity in Maize Hybrid Breeding

2021 ◽  
Author(s):  
Chimwemwe Josia ◽  
Kingstone Mashingaidze ◽  
Assefa B. Amelework ◽  
Aleck Kondwakwenda ◽  
Cousin Musvosvi ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Snp Markers ◽  
Hybrid Breeding ◽  
Maize Breeding ◽  
Genetic Purity ◽  
Maize Hybrid ◽  
Dh Lines ◽  
High Level

AbstractAssessment of genetic purity of inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. Maximum genetic distance of (0.56) was observed for CKDHL0089, CML443 and CB323, while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% were had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.

scholarly journals Classical Genetic and Quantitative Trait Loci Analyses of Heterosis in a Maize Hybrid Between Two Elite Inbred Lines

Genetics ◽  
2007 ◽  
Vol 176 (1) ◽  
pp. 625-644 ◽  
Author(s):  
Elisabetta Frascaroli ◽  
Maria Angela Canè ◽  
Pierangelo Landi ◽  
Giorgio Pea ◽  
Luca Gianfranceschi ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Inbred Lines ◽  
Maize Hybrid ◽  
Classical Genetic ◽  
Trait Loci

Performance of Indirect ELISA for Maize Hybrid Purity Test

2013 ◽  
Vol 781-784 ◽  
pp. 1741-1744
Author(s):  
Yue Hui Liu ◽  
Jun Wang ◽  
Xin Chao Yang ◽  
Na Xin Sun ◽  
Li Hua Xu
Keyword(s):  
Pure Water ◽  
Inbred Lines ◽  
Test Methods ◽  
Whole Grain ◽  
Maize Hybrid ◽  
Hybrid Purity ◽  
Purity Test ◽  
Detectable Rate ◽  
Large Crop ◽  
Single Blind

Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.

scholarly journals SNP-based assessment of genetic purity and diversity in maize hybrid breeding

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0249505
Author(s):  
Chimwemwe Josia ◽  
Kingstone Mashingaidze ◽  
Assefa B. Amelework ◽  
Aleck Kondwakwenda ◽  
Cousin Musvosvi ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Snp Markers ◽  
Hybrid Breeding ◽  
Maize Breeding ◽  
Genetic Purity ◽  
Maize Hybrid ◽  
Dh Lines ◽  
High Level

Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. A maximum genetic distance of (0.56) was observed between inbred lines CKDHL0089 and CML443 while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.

scholarly journals Inbred lines of different cycles of selection as donors of favourable alleles for the improvement of F1 maize hybrids

Genetika ◽  
2010 ◽  
Vol 42 (2) ◽  
pp. 339-348 ◽  
Author(s):  
Mile Secanski ◽  
Goran Todorovic ◽  
Gordana Surlan-Momirovic ◽  
Tomislav Zivanovic ◽  
Milosav Babic
Keyword(s):  
Grain Yield ◽  
Inbred Lines ◽  
Maize Hybrid ◽  
Maize Hybrids ◽  
Estimated Parameters ◽  
Yield Trait ◽  
Single Cross ◽  
Synthetic Population

Eight BSSS and BSCB1 inbred lines of different cycles of selection were studied with the aim to evaluate which inbreds had the highest relative values of favourable alleles for the improvement of the grain yield trait in an elite single cross maize hybrid. Based on estimated parameters ?G, UBND, PTC and NI it was determined that the inbreds B73 (C5) and B84 (C7), originating form the synthetic population BSSS had highest number of favourable alleles for the improvement of the grain yield. Both these inbreds belong to the later cycles of recurrent selections and proved to be better donors of favourable alleles than inbreds belonging to the earlier cycles of selection. The rank correlations of used parameters were positive and highly significant, while the highest values of correlations for yield were determined between ?G and NI, that is PTC and NI. The highest grain yield was recorded in the hybrid ZPL2 x B73, which also had the highest value of heterosis (174.9%) in the trial for estimation of loci relative values.

scholarly journals Interchromosomal Recombination in Zea mays

Genetics ◽  
1998 ◽  
Vol 150 (3) ◽  
pp. 1229-1237 ◽  
Author(s):  
Weiming Hu ◽  
Marja C P Timmermans ◽  
Joachim Messing
Keyword(s):  
Zea Mays ◽  
Plant Development ◽  
Repetitive Element ◽  
Inbred Lines ◽  
Single Base ◽  
Conserved Sequences ◽  
Somatic Recombination ◽  
New Alleles ◽  
Zein Gene

Abstract A new allele of the 27-kD zein locus in maize has been generated by interchromosomal recombination between chromosomes of two different inbred lines. A continuous patch of at least 11,817 bp of inbred W64A, containing the previously characterized Ra allele of the 27-kD zein gene, has been inserted into the genome of A188 by a single crossover. While both junction sequences are conserved, sequences of the two homologs between these junctions differ considerably. W64A contains the 7313-bp-long retrotransposon, Zeon-1. A188 contains a second copy of the 27-kD zein gene and a 2-kb repetitive element. Therefore, recombination results in a 7.3-kb insertion and a 14-kb deletion compared to the original S+A188 allele. If nonpairing sequences are looped out, 206 single base changes, frequently clustered, are present. The structure of this allele may explain how a recently discovered example of somatic recombination occurred in an A188/W64A hybrid. This would indicate that despite these sequence differences, pairing between these alleles could occur early during plant development. Therefore, such a somatically derived chimeric chromosome can also be heritable and give rise to new alleles.

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