Performance of Indirect ELISA for Maize Hybrid Purity Test

2013 ◽  
Vol 781-784 ◽  
pp. 1741-1744
Author(s):  
Yue Hui Liu ◽  
Jun Wang ◽  
Xin Chao Yang ◽  
Na Xin Sun ◽  
Li Hua Xu
Keyword(s):  
Pure Water ◽  
Inbred Lines ◽  
Test Methods ◽  
Whole Grain ◽  
Maize Hybrid ◽  
Hybrid Purity ◽  
Purity Test ◽  
Detectable Rate ◽  
Large Crop ◽  
Single Blind

Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.

Evaluation of inbred lines as sources of new alleles for improving elite maize hybrid

2006 ◽  
Vol 34 (2-3) ◽  
pp. 941-948 ◽  
Author(s):  
Tomislav Zivanovic ◽  
Savo Vuckovic ◽  
Slaven Prodanovic ◽  
Goran Todorovic
Keyword(s):  
Inbred Lines ◽  
Maize Hybrid ◽  
New Alleles

Advances of melon hybrid purity test

2018 ◽  
pp. 115-118
Author(s):  
C. Li ◽  
Y.P. Sun ◽  
Y. Yang ◽  
C. Xu ◽  
X.F. Liao
Keyword(s):  
Hybrid Purity ◽  
Purity Test

scholarly journals Keragaman Genetik Inbrida Jagung QPM dan Normal Berbasis Marka Mikrosatelit dan Hubungannya dengan Penampilan Hibrida

2016 ◽  
Vol 4 (2) ◽  
pp. 77
Author(s):  
Marcia B. Pabendon ◽  
M. Azrai ◽  
M. J. Mejaya ◽  
Sutrisno Sutrisno
Keyword(s):  
Grain Yield ◽  
Field Trials ◽  
Inbred Lines ◽  
Heterotic Group ◽  
Maize Hybrid ◽  
Normal Maize ◽  
Single Cross ◽  
Upgma Cluster Analysis ◽  
Hybrid Program ◽  
And Performance

<p>Information on genetic divergence of<br />inbred lines and performance of the hybrids developed from<br />the lines is a great value in maize hybrid program. A study<br />was conducted to evaluate genetic diversity of six QPM and<br />five normal maize inbred lines, to determine the relationship<br />between genetic distance based on SSR markers and the<br />grain yield of single cross hybrid, and to get information<br />promising hybrid from the single cross of QPM hybrid.<br />Twenty four polymorphic primers that covered the 10 maize<br />chromosomes were used to fingerprint the lines, detecting<br />in 94 alleles (average of 3.9 and a range of 2-6 alleles per<br />locus). Genetic divergences were determined using the<br />Jaccard’s similarity coefficient, and a dendrogram was<br />constructed using the UPGMA. Cluster analysis divided the<br />inbreds into two clusters that were confirmed by principal<br />coordinate analysis. Two promising QPM hybrids that are<br />crossed from different heterotic group were found. The<br />estimated value of simple correlations (r) of GDs with the<br />gain yield of single cross hybrid was negatif (-0.07). There is<br />a need to conduct more field trials to obtain more accurate<br />correlations, particularly in a practical utility for predicting<br />maize hybrid performance for grain yield.</p>

scholarly journals SNP- Based Assessment of Genetic Purity and Diversity in Maize Hybrid Breeding

2021 ◽  
Author(s):  
Chimwemwe Josia ◽  
Kingstone Mashingaidze ◽  
Assefa B. Amelework ◽  
Aleck Kondwakwenda ◽  
Cousin Musvosvi ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Snp Markers ◽  
Hybrid Breeding ◽  
Maize Breeding ◽  
Genetic Purity ◽  
Maize Hybrid ◽  
Dh Lines ◽  
High Level

AbstractAssessment of genetic purity of inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. Maximum genetic distance of (0.56) was observed for CKDHL0089, CML443 and CB323, while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% were had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.

scholarly journals Classical Genetic and Quantitative Trait Loci Analyses of Heterosis in a Maize Hybrid Between Two Elite Inbred Lines

Genetics ◽  
2007 ◽  
Vol 176 (1) ◽  
pp. 625-644 ◽  
Author(s):  
Elisabetta Frascaroli ◽  
Maria Angela Canè ◽  
Pierangelo Landi ◽  
Giorgio Pea ◽  
Luca Gianfranceschi ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Inbred Lines ◽  
Maize Hybrid ◽  
Classical Genetic ◽  
Trait Loci

scholarly journals Optimization of sampling size for DNA-based PCR assay for hybrid purity test in the brinjal, Solanum melongena

2018 ◽  
Vol 11 (3) ◽  
pp. 496-504
Author(s):  
Arpita Pattanaik ◽  
Lakshmana Reddy D.C ◽  
T.H. Singh ◽  
P. Pandiyaraj ◽  
Aswath Chennareddy
Keyword(s):  
Solanum Melongena ◽  
Pcr Assay ◽  
Hybrid Purity ◽  
Purity Test

scholarly journals Research on Computer Aided Test System for Sealing Characteristics of Hydraulic Support Pure Water Hydraulic Cylinder

2021 ◽  
Vol 2143 (1) ◽  
pp. 012048
Author(s):  
Yuanzhi Huang
Keyword(s):  
Pure Water ◽  
Hydraulic Cylinder ◽  
Test System ◽  
Test Methods ◽  
Hydraulic Support ◽  
Complex Process ◽  
Safety And Reliability ◽  
Water Hydraulic ◽  
Low Efficiency ◽  
And Function

Abstract The sealing characteristics of the hydraulic cylinder in the hydraulic support will have an momentous implication on the safety and reliability of the whole system. The traditional test methods of the sealing characteristics of hydraulic support pure water hydraulic cylinder have many shortcomings, such as single means, complex process, poor accuracy and low efficiency. Based on this, this paper first analyzes the sealing principle and function of hydraulic support pure water hydraulic cylinder, then studies the CAT system of hydraulic support, and finally gives the design and utilization process of CAT system for sealing characteristics of hydraulic support pure water hydraulic cylinder.

scholarly journals Performance of provitamin A- quality protein maize inbred lines and derived hybrids in contrasting environments

2019 ◽  
Author(s):  
Ebenezer Obeng-Bio ◽  
Baffour Badu-Apraku ◽  
Beatrice Elohor Ifie ◽  
Agyemang Danquah ◽  
Essie Blay ◽  
...  
Keyword(s):  
Grain Yield ◽  
Central Africa ◽  
Inbred Lines ◽  
Provitamin A ◽  
Quality Protein Maize ◽  
Whole Grain ◽  
Maize Inbred Lines ◽  
Early Maturing ◽  
West And Central Africa ◽  
The Relationship

Abstract Background Early maturing provitamin A (PVA) quality protein maize (QPM) inbred lines with tolerance to drought and low-N are needed to develop superior hybrids in West and Central Africa (WCA). This study aimed to (i) identify inbred lines that combined drought and low-N tolerance with increased levels of PVA and tryptophan and (ii) assess the relationship among PVA carotenoids, tryptophan and grain yield. Sixty-four inbred lines plus six inbred checks were evaluated under induced drought, low-N and optimal conditions in 2016 and 2017 in Nigeria. The inbred lines were assayed for PVA and tryptophan contents. Results Thirty-three of the lines were found to be tolerant to drought and low-N. Ninety percent of the inbred lines had tryptophan contents > 0.075 % per sample in whole grain substantiating the presence of the QPM trait in the inbred lines. Inbred lines TZEIORQ 55 and TZEIORQ 29 combined high PVA contents (15.38 and 12.10μg g-1, respectively) with low-N tolerance while nine inbred lines combined moderate PVA levels (5.06 – 8.34μg g-1) with drought and low-N tolerance. Conclusions These maize inbred lines could be utilized to develop superior drought and low-N tolerant hybrids and synthetics with elevated levels of PVA and tryptophan for WCA. The correlations observed among grain yield, PVA and tryptophan of the lines were not significant suggesting that these traits could be improved independently.

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