Simultaneous high-performance thin-layer chromatographic determination of indole acetic acid, indole butyric acid, and Absisic acid inin vitroseedling of watermelon exposed to heavy metals

2012 ◽  
Vol 25 (2) ◽  
pp. 108-111 ◽  
Author(s):  
Ersin Kilinc ◽  
Veysi Okumus ◽  
M. Düz ◽  
Firat Aydin
2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao

2010 ◽  
Vol 7 (3) ◽  
pp. 1113-1119
Author(s):  
Baghdad Science Journal

This study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .


1979 ◽  
Vol 62 (1) ◽  
pp. 201-202 ◽  
Author(s):  
Robert D Stubblefield

Abstract Clearly defined zones of citrinin can be obtained on thin layer chromatographic (TLC) plates and measured by fluorodensitometry. Silica gel plates were prepared as a slurry with aqueous 0.05M Na2EDTA (ethylenediaminetetraacetic acid), spread at 0.5 mm wet thickness, and activated at 105°C for 1 hr. Plates were developed in acetic acid-benzene (5+95). The limit of detection was 10 ng citrinin/zone. Densitometric analysis (365 nm excitation, 505 nm emission) revealed that a linear relationship exists for levels of 10 ng to at least 100 ng/zone wtih a coefficient of variation of ±5%.


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