scholarly journals Identification of dengue viruses in naturally infected Aedes aegypti females captured with BioGents (BG)-Sentinel traps in Manaus, Amazonas, Brazil

2013 ◽  
Vol 46 (2) ◽  
pp. 221-222 ◽  
Author(s):  
Regina Maria Pinto de Figueiredo ◽  
Maria Paula Gomes Mourão ◽  
Yasmin Emile Conte Abi-Abib ◽  
Cintia Mara de Oliveira ◽  
Rosemary Roque ◽  
...  
2015 ◽  
Vol 2 (1) ◽  
pp. 86
Author(s):  
Rike Oktarianti ◽  
Kartika Senjarini ◽  
Fatchiyah . ◽  
Aulani’am .

The saliva of hematophagous arthropods contains a complex mixture of biologically active proteins. These proteins may modify hemostatic responses and induce both cellular immunity and the production of specific antibodies, and thus influence the transmission of its pathogens from arthropods vector to human host. Aedes aegypti is the main vector for transmission of dengue viruses into human. The objective of this study was to examine individual human sera response against protein extracts from salivary gland of Ae aegypti that mediate the infection of dengue viruses. We did a cross reaction test of human sera from healthy people in endemic and non-endemic area, and dengue patients againts SGE of Ae. aegypti to distinguish and to identify the immunogenic proteins using Western Blot Analysis. About 15 protein bands of SGE from Ae. aegypti ranging from 15 kDa up to 255 kDa were identified on 12% SDS-PAGE. Seven dominant bands were detected, i.e ~255, 56, 42, 31, 27, 26 and 15 kDa. Two immunogenic proteins, as represented by two bands, i.e. ~31 and 56 kDa were found only in samples from people who were previously exposed to mosquitoes bites, and not in people who had not been exposed. Therefore, these immunogenic salivary proteins may serve as indicators for the immune response in human against protein from salivary gland of Ae. aegypti.Keywords: immunogenic proteins, salivary gland, Aedes aegypti


2010 ◽  
Vol 19 (6) ◽  
pp. 753-763 ◽  
Author(s):  
G. Mathur ◽  
I. Sanchez-Vargas ◽  
D. Alvarez ◽  
K. E. Olson ◽  
O. Marinotti ◽  
...  

1979 ◽  
Vol 28 (6) ◽  
pp. 1045-1052 ◽  
Author(s):  
D. J. Gubler ◽  
S. Nalim ◽  
J. Sulianti Saroso ◽  
H. Saipan ◽  
R. Tan

2013 ◽  
Vol 108 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Tatiana Mingote Ferreira de Ázara ◽  
Carolin Marlen Degener ◽  
Rosemary Aparecida Roque ◽  
Jörg Johannes Ohly ◽  
Martin Geier ◽  
...  

Author(s):  
Robert H. Whitehead ◽  
Thomas M. Yuill ◽  
Douglas J. Gould ◽  
Phinit Simasathien

2018 ◽  
Vol 71 (1) ◽  
pp. 58-61 ◽  
Author(s):  
Kris Cahyo Mulyatno ◽  
Tomohiro Kotaki ◽  
Subagyo Yotopranoto ◽  
Etik Ainun Rohmah ◽  
Siti Churotin ◽  
...  

2010 ◽  
Vol 47 (4) ◽  
pp. 657-663 ◽  
Author(s):  
Tamara S. Ball ◽  
Scott R. Ritchie

Abstract The BG-Sentinel (BGS) trap uses visual and olfactory cues as well as convection currents to attract Aedes aegypti (L.). The impact of the visual environment on trapping efficacy of the BGS trap for Ae. aegypti was investigated. Four- to 5-d nulliparous female and male Ae. aegypti were released into a semicontrolled room to evaluate the effect of the presence, reflectance, and distribution of surrounding harborage sites on BGS trapping efficacy. Low-reflective (dark) harborage sites near the BGS had a negative effect on both male and nulliparous female recapture rates; however, a more pronounced effect was observed in males. The distribution (clustered versus scattered) of dark harborage sites did not significantly affect recapture rates in either sex. In a subsequent experiment, the impact of oviposition sites on the recapture rate of gravid females was investigated. Although gravid females went to the oviposition sites and deposited eggs, the efficacy of the BGS in recapturing gravid females was not compromised. Ae. aegypti sampling in the field will mostly occur in the urban environment, whereby the BGS will be among oviposition sites and dark harborage areas in the form of household items and outdoor clutter. In addition to understanding sampling biases of the BGS, estimations of the adult population size and structure can be further adjusted based on an understanding of the impact of dark harborage sites on trap captures. Outcomes from this suite of experiments provide us with important considerations for trap deployment and interpretation of Ae. aegypti samples from the BGS trap.


Sign in / Sign up

Export Citation Format

Share Document