scholarly journals Mortality of Conotrachelus humeropictus in response to combined application of the nematode Steinernema brazilense and the fungus Beauveria bassiana

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Lucas Detogni Simi ◽  
Luís Garrigós Leite ◽  
Olzeno Trevisan ◽  
José Nilton Medeiros Costa ◽  
Leandro Ezequiel Oliveira ◽  
...  

ABSTRACT: The fruit borer Conotrachelus humeropictus is one of the most important pests of the cupuaçu crop, and can promote yield losses of around 50%. The present study involved the selection of Beauveria bassiana isolates on Conotrachelus psidii larvae, which was used as test insect due to its taxonomic closeness to C. humeropictus. In order to assess their interaction using combined application, we studied the ability of the nematode to carry conidia of B. bassiana and Metarhizium anisopliae to soil layers. This study also aimed to establish a biological control method for cupuaçu borer with the use of the fungus B. bassiana at 10 kg/ha and the entomopathogenic nematode Steinernema brazilense at 1, 3, 9, and 27 IJ/cm2, applied as single and combined treatments. In the selection of B. bassiana strains for C. psidii, the IBCB 276 and IBCB 165 isolates were the most promising ones, causing 86 and 84% mortality, respectively. The IBCB 276 strain was applied in the field to control C. humeropictus. In the study of carriage of conidia in soil, the nematode S. brazilense was found to be capable of carrying conidia of B. bassiana to deep 7 to 10 cm soil layers. This was not observed with M. anisopliae, found only in the topsoil. In field trials against C. humeropictus larvae, B. bassiana promoted 15.6% mortality. The nematode promoted 60.0% mortality at the highest concentration used, and their combined use with B. bassiana (10 kg/ha) increased mortality to 65.6%, with an additive interaction observed between the two entomopathogens.

2002 ◽  
Vol 59 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Everardo César Filho ◽  
Edmilson Jacinto Marques ◽  
Reginaldo Barros

The cotton leafworm, Alabama argillacea (Huebner, 1818) (Lepidoptera: Noctuidae), is considered to be one of the key pests in herbaceous cotton (Gossypium hirsutum L. r. latifolium Hutch) cropping, with constant occurrence in all cotton-growing states of Brazil. In this study Metarhizium anisopliae and Beauveria bassiana isolates were screened and evaluated for pathogenicity against Alabama argillaceae. Initially, a screening of ten isolates of each fungus in a concentracion of 10(8) conidia mL-1, was carried out on 3rd instar larvae of A. argillacea. Further studies were conducted to determine the pathogenicity and virulence of six and seven isolates of M. anisopliae and B. bassiana, respectively, against 3rd instar larvae of A. argillacea and using the concentrations of 10(6), 10(7), 10(8), and 10(9) conidia mL-1. The experiments were carried out in Recife, PE, Brazil, at 27 ± 2ºC, RH 70 ± 5% and a photophase of 12 hours. Mortalities caused by M. anisopliae isolate at the different concentrations ranged from 4.5 to 91.2%, the highest mortality percentage being found for the isolate 1189 at 10(9) conidia mL-1. The isolate 645 of B. bassiana caused the highest mortality at the highest concentration, followed by isolates 634, 604, and IPA 198. The lowest lethal time for B. bassiana and M. anisopliae, was achieved by the isolates 483 (4.1 days) and 1189 (2.0 days), respectively. The isolates 1189, 1022 e 866 of M. anisopliae and 483, IPA198 and 604 of B. bassiana, at 10(8) e 10(9) conidia mL-1 are promissing for use the integrated control of A. argillacea larvae, but M. anisopliae seems more effective.


2008 ◽  
Vol 97 (2) ◽  
pp. 83-90 ◽  
Author(s):  
Patricia Helena Santoro ◽  
Pedro Manuel Oliveira Janeiro Neves ◽  
Talita Moretto Alexandre ◽  
Daniele Sartori ◽  
Luis Francisco Angeli Alves ◽  
...  

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5857
Author(s):  
Brandy J. Johnson ◽  
Anthony P. Malanoski ◽  
Jeffrey S. Erickson

This review describes an ongoing effort intended to develop wireless sensor networks for real-time monitoring of airborne targets across a broad area. The goal is to apply the spectrophotometric characteristics of porphyrins and metalloporphyrins in a colorimetric array for detection and discrimination of changes in the chemical composition of environmental air samples. The work includes hardware, software, and firmware design as well as development of algorithms for identification of event occurrence and discrimination of targets. Here, we describe the prototype devices and algorithms related to this effort as well as work directed at selection of indicator arrays for use with the system. Finally, we review the field trials completed with the prototype devices and discuss the outlook for further development.


2015 ◽  
Vol 713-715 ◽  
pp. 1556-1559
Author(s):  
Ning Wang ◽  
De Chao Zhang

The inductance and the capacitance on DC side are two important parameter of SVG. In this paper, the effect of inductance and capacitance on DC sides’ voltage control method of SVG is addressed.


1970 ◽  
Vol 68 (2) ◽  
pp. 221-232 ◽  
Author(s):  
R. J. Gilbert

SUMMARYThere is no official scheme for testing disinfectants and detergent/disinfectants for use in the retail food trade and few recommended procedures have been given for the cleaning of equipment with these agents. Therefore, field trials were carried out in a large self-service store. Comparisons were made of the various cleaning efficiencies, as determined by bacterial plate counts, of detergent and disinfectant solutions and machine cleaning oils applied with either clean cloths or disposable paper towels to items of equipment. The most satisfactory results were always obtained when anionic detergent (0·75 % w/v) and hypochlorite (200 p.p.m. available chlorine) solutions were applied in a ‘two-step’ procedure.Tests were made to compare the calcium alginate swab-rinse and the agar sausage (Agaroid) techniques for the enumeration of bacteria on stainless steel, plastic, formica and wooden surfaces before and after a cleaning process. Although recovery rates were always greater by the swab-rinse technique, the agar sausage technique was considered to be a useful routine control method for surface sampling.


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