In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects

In vitro culture systems based on liquid culture media are considered to be more effective than semisolid culture medium systems. Liquid culture media systems provide better nutrient availability for plant tissues, easier culture handling, and the potential for scaling up and automation. However, in vitro liquid culture requires more careful handling due to the potential for contamination and the possibility of negative effects, such as hyperhydricity or vitrification, that hinder the growth and development of the plant material. Temporary immersion bioreactors have emerged as a workable alternative for capturing the benefits of liquid media, though semisolid systems are still traditional. Many studies have shown that silicon (Si) is a beneficial plant nutrient. Silicon might have a positive effect in both semisolid and liquid in vitro systems. The objective of this study was to evaluate the effect of silicon on the micropropagation and acclimatization of banana plants cultivated in vitro by comparing liquid temporary immersion bioreactor technology and semisolid traditional culture systems. Different silicon concentrations (0 and 1 mL L-1) and culture systems (liquid temporary immersion bioreactor and semisolid traditional culture) were evaluated over a 36-day period. The growth characteristics plant size, fresh and dry weight, and number and length of leaves and roots were evaluated. After the 36-day in vitro growth period, plants were transferred to a greenhouse for acclimatization and were evaluated after 30 days for the same growth characteristics used in the in vitro studies. The temporary immersion bioreactor system resulted in greater growth of banana plants compared to the traditional semisolid system. Temporary immersion bioreactors also showed a positive interaction with Si and resulted in higher values for all growth characteristics in the acclimatization phase.

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Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452019102 ◽

2019 ◽

Vol 41 (1) ◽

Author(s):

Samila Silva Camargo ◽

Leo Rufato ◽

Maicon Magro ◽

André Luiz Kulkamp de Souza

Keyword(s):

Liquid Medium ◽

Culture Medium ◽

Culture Media ◽

Time Factors ◽

Efficient Technique ◽

Control Treatment ◽

Solid Culture ◽

Temporary Immersion ◽

Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

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In vitro multiplication and rooting of grapevine: Culture media and plant growth regulators

Plant Cell Culture & Micropropagation ◽

10.46526/pccm.2019.v15i1.136 ◽

2019 ◽

Vol 15 (1) ◽

pp. 1-7

Author(s):

Daniele Camargo Nascimento ◽

Maximiliano Dini ◽

Norton Victor Sampaio ◽

Márcia Wulff Schuch

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

In Vitro Multiplication

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Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor

Revista Fitos ◽

10.32712/2446-4775.2020.799 ◽

2020 ◽

Vol 14 (01) ◽

pp. 45-55

Author(s):

Simone Da Silva ◽

Danielle Cardoso Alencar ◽

Paulo José Coelho Benevides ◽

Spartaco Astolfi-Filho

Keyword(s):

Natural Populations ◽

Root Induction ◽

Relative Area ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Mean Values ◽

Solid Media ◽

Medicinal Properties ◽

Semi Solid

Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L-1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively.

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In Vitro Multiplication of Potato (Solanum tuberosum L.) cv. Cristal under Different MS Salt and Sucrose Concentrations

HortScience ◽

10.21273/hortsci.32.3.471e ◽

1997 ◽

Vol 32 (3) ◽

pp. 471E-471

Author(s):

Gerson R. de L. Fortes ◽

Luciana B. Andrade ◽

Marisa de F. Oliveira ◽

Nilvane T.G. Müller ◽

Janine T. C. Faria

Keyword(s):

Culture Media ◽

Solanum Tuberosum L ◽

Sucrose Concentration ◽

Sugar Content ◽

Potato Cultivar ◽

In Vitro Multiplication ◽

Full Strength ◽

High Dry Matter ◽

The Media

The potato cultivar Cristal has recently been released by the CPACT/EMBRAPA Breeding Program. Such cultivar was selected for having high dry matter and low sugar content, which makes it desirable for the chip industry. However, this is a recalcitrant cultivar as far as in vitro multiplication is concerned. The aim of this work was to improve the rate of multiplication for this cultivar when it was submitted to different MS salt and sucrose concentrations in the culture media. Two-bud microcuttings were inoculated in test tubes (20 × 150) mm with 10 ml MS media at 3/4-, 1/2-, and full-strength and MS vitamins added to: myo-inositol (100 mg·L–1), agar (7.0 g·L–1) and sucrose as follows: 10, 20 and 30 g·L-1. Each treatment was repeated eight times and each replicate had eight explants. After inoculation the whole material was kept in a growth room at 25 ± 2°C, 16-hr photoperiod and 2000 lux. The evaluation was done 35 days later. It was found and increase in the number of buds as the sucrose concentration in the media decreased. As far as MS salts are concerned no difference in bud number was observed. The rate of multiplication was slightly higher for MS media at full strength and sucrose at low concentration (10 g·L–1). This treatment could be recommended for this cultivar.

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Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽

10.21273/hortsci.45.7.1093 ◽

2010 ◽

Vol 45 (7) ◽

pp. 1093-1098 ◽

Cited By ~ 9

Author(s):

Jericó J. Bello-Bello ◽

Adriana Canto-Flick ◽

Eduardo Balam-Uc ◽

Eunice Gómez-Uc ◽

Manuel L. Robert ◽

...

Keyword(s):

Shoot Elongation ◽

Nodal Segments ◽

High Survival ◽

Shoot Induction ◽

Capsicum Chinense ◽

Temporary Immersion Bioreactor ◽

In Vitro Proliferation ◽

Temporary Immersion ◽

Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

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In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor

Plant Science ◽

10.1016/s0168-9452(03)00064-5 ◽

2003 ◽

Vol 165 (3) ◽

pp. 463-470 ◽

Cited By ~ 47

Author(s):

S.M.A. Zobayed ◽

P.K. Saxena

Keyword(s):

Shoot Regeneration ◽

Hypericum Perforatum ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

St John’S Wort ◽

St John's Wort ◽

Hypericum Perforatum L ◽

Prolific Shoot

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Biorreator na micropropagação de abacaxi ornamental

Ornamental Horticulture ◽

10.14295/oh.v24i2.1181 ◽

2018 ◽

Vol 24 (2) ◽

pp. 182-187

Author(s):

Caroline Oliveira Reis ◽

Adriano Bortolotti Silva ◽

Paulo Roberto Landgraf ◽

Jessica Azevedo Batisita ◽

Guilherme Antonio Jacome

Keyword(s):

Natural Ventilation ◽

Ananas Comosus ◽

Basic Medium ◽

In Vitro Cultivation ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Time Period ◽

Culture Growth ◽

Short Time

Ornamental pineapple is a hard plant with significant landscaping value. Typically, conventional propagation is performed by clump division with low yields, and may even spread diseases. Plant tissue culture is viable, yielding plants with a high phytosanitary and genetic quality over a short time period. This study aimed to verify the in vitro multiplication of ornamental pineapple plants (Ananas comosus var. bracteatus L.) in different micropropagation systems, in association with BAP concentrations. Plants with about 2 cm were used, transplanted to the different treatments: bioreactor, natural ventilation and conventional micropropagation, combined with 3 BAP concentrations (0, 1 and 2 mg L-1). The basic medium used consisted of MS salts. The highest number of shoots and in vitro culture growth were obtained with the use of bioreactor and culture medium containing 2 mg L-1 BAP. The temporary immersion bioreactor allows air renewal inside the bottles, leading to a better performance of in vitro cultivation of ornamental pineapple, when compared to conventional micropropagation.

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in Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media

Science Journal of University of Zakho ◽

10.25271/2014.2.2.212 ◽

2014 ◽

Vol 2 (2) ◽

pp. 310-322

Author(s):

Layla AL-Mizory ◽

Ameena Hassan

Keyword(s):

Culture Media ◽

In Vitro Multiplication ◽

Photinia X Fraseri

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