scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

2021 ◽  
pp. 113-120
Author(s):  
A.L. Arruda ◽  
F.R. Nerbass ◽  
A.A. Kretzschmar ◽  
L. Rufato ◽  
A.J. Posser ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Bazı Turunçgil Anaçlarının Klasik ve Yeni Nesil Doku Kültürü Teknikleri ile Mikroçoğaltımı

2019 ◽  
Vol 7 (9) ◽  
pp. 1469
Author(s):  
Melike Cengiz ◽  
Yıldız Aka Kaçar
Keyword(s):  
Culture Medium ◽  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Solid Culture ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Rooting Medium ◽  
Solid Culture Medium ◽  
Isopentenyl Adenine ◽  
Bioreactor System

In this study, micropropagation and rooting of ‘Tuzcu 31-31 sour orange’ and ‘C-35 citrange’ citrus rootstocks were conducted by comparing with Plantform temporary immersion bioreactor system and traditional solid culture. Murashige and Skoog Medium (MS) and Woody Plant Medium (WPM) supplemented with 6-Benzylaminopurine (BAP) (0, 1.0, 2.0 mg L-1), Kinetin (KIN) (0, 0.5, 1.0 mg L-1) and 2-Isopentenyl adenine (2IP) (0, 1.0, 2.0 mg L-1) were used in solid culture experiments. For solid culture rooting experiments, MS, ½ MS and WPM media supplemented with different concentrations of 1-Naphthaleneacetic acid (NAA) (0, 0.5, 1.0, 2.0 mg L-1) and Indole-3-butyric acid (IBA) (0, 0.5, 1.0, 2.0 mg L-1) were used. In both genotypes, the best micropropagation and rooting results were obtained from MS medium containing 2.0 mg L-1 BAP and ½ MS nutrient medium containing 0.5 mg L-1 NAA, respectively. Plantform bioreactor system was studied with the best medium content determined for micropropagation and rooting. As a result of the study, Plantform system gave better results in terms of plant quality in the micropropagation medium for both genotypes. Plantform system in rooting medium was found to be more advantageous than solid culture medium. As a result of the screening with SSR markers, it was determined that there was no somaclonal variation in the plants micropropagated and rooted in Plantform system.

scholarly journals Elicitation of Phenylpropanoids in Maqui (Aristotelia chilensis [Mol.] Stuntz) Plants Micropropagated in Photomixotrophic Temporary Immersion Bioreactors (TIBs).

2021 ◽  
Author(s):  
Giulia E Trentini ◽  
Makarena Rojas ◽  
Daniela Gajardo ◽  
Débora Alburquenque ◽  
Evelyn Villagra ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Plants ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Fresh Weight ◽  
Temporary Immersion ◽  
Air Treatment ◽  
The Third ◽  
Aristotelia Chilensis

Abstract A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m− 2s− 1. The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO2 do not differ significantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air. Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not significant differences in polyphenols and anthocyanin contents were verified between the treatments of maqui in TIBs + ABA and controls. The non-significant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no significant differences by the ORAC test from day 5 to day 15 of the study.

scholarly journals Effects of Preharvest Methyl Jasmonate Elicitation and Electrical Stimulation on Camptothecin Production by In Vitro Plants of Ophiorrhiza ridleyana Craib

2021 ◽  
Vol 11 (10) ◽  
pp. 4555
Author(s):  
Supakit Pisitpaibool ◽  
Suchada Sukrong ◽  
Kijchai Kanjanaprapakul ◽  
Muenduen Phisalaphong
Keyword(s):  
Tissue Culture ◽  
Electrical Stimulation ◽  
Methyl Jasmonate ◽  
Culture Medium ◽  
Solid Culture ◽  
Direct Electric Current ◽  
Solid Culture Medium ◽  
The Mean ◽  
Stems And Leaves

To enhance plant camptothecin (CPT) production in vitro, 5-month-old Ophiorrhiza ridleyana Craib plant cultures were treated with solutions of methyl jasmonate (MeJA) dissolved in ethanol, which were applied to the surface of the solid culture medium. It was demonstrated that the maximum CPT content in the tissue-cultured plants was achieved after 12 h elicitation with 50 µM MeJA. The mean CPT contents in roots and stems were 50.8 and 67.0 µg/g DW, respectively, which were approximately 1.8- and 2.6-fold higher, respectively, than those of the control. However, MeJA elicitation showed no significant effect on CPT accumulation in O. ridleyana leaves. Moreover, it was found that direct electric current (DC) stimulation also significantly increased CPT accumulation in O. ridleyana. The treatment with DC at 20 mA for 3 min of stimulation enhanced 3-fold the CPT content in roots, stems, and leaves to 41.9, 36.0 and 19.6 µg/g DW, respectively, which were approximately 1.5-, 1.7- and 1.4-fold higher, respectively, as compared to those of the control. The results demonstrate that preharvest treatment by MeJA elicitation and electrical stimulation can be beneficial for secondary metabolite production of CPT in tissue-culture plants of O. ridleyana.

scholarly journals Feasibility of the new method for orchid in vitro rooting using liquid and chemical sterilized culture medium under different sucrose concentration

2019 ◽  
Vol 25 (3) ◽  
pp. 263-269
Author(s):  
Beatriz Cristina de Oliveira ◽  
Maria Eduarda Barboza Souza de Oliveira ◽  
Jean Carlos Cardoso
Keyword(s):  
Liquid Medium ◽  
Polyurethane Foam ◽  
Culture Medium ◽  
New Technologies ◽  
Culture Media ◽  
Sucrose Concentration ◽  
In Vitro Rooting ◽  
Total Period ◽  
Semi Solid

Abstract The in vitro propagation of orchids is the only commercial large scale technique to obtain healthy and high quality plantlets with clonal origin. The use of new technologies in plant tissue culture systems could lead to efficiency increases and costs reduction of micropropagation systems. The main actual micropropagation system is based on semi-solid culture media solidified using agar, followed by sterilization using autoclaving, and cultivation under photomixotrophic conditions using sucrose as main source of energy to plant in vitro culture. We proposed in this study the use of new micropropagation system using chemical sterilized liquid medium using polyurethane foam as support and LED source of light in rooting stage of Miltassia ‘Shelobie Tolkien’. Thus, the objective of this research was to test different concentrations of sucrose, comparing the conventional semi-solid agar-based culture medium (control) and the use of liquid medium with polyurethane foam support. The following sucrose concentrations were used: 0, 7.5, 15, 22.5 and 30 g L-1. The experiment was conducted in a 2 × 5 factorial, in a completely randomized design with ten replications each over a total period of 105 days of cultivation. The chemical sterilization using ClO2 showed 100% of decontamination in all treatments. The use of liquid media with polyurethane foam showed better results than plants cultivated in agar medium, and can be used for replace agar-based for orchid in vitro rooting.

Notes sur la production, in vitro, de sporophores par Pholiota aurivella

1971 ◽  
Vol 49 (4) ◽  
pp. 567-572 ◽  
Author(s):  
André Lavallée ◽  
Marcel Lortie
Keyword(s):  
Environmental Conditions ◽  
Nutrient Concentration ◽  
Culture Medium ◽  
Light Stimulus ◽  
Light Sources ◽  
Malt Extract ◽  
Solid Culture ◽  
Solid Culture Medium

The influence of light and other environmental conditions on the sporophore production was tested for Pholiota aurivella in the laboratory. Among different light sources tested, only sunlight, through the window, led to the production of complete sporophores in culture. The light stimulus must be given within the first 14 days following mycelium implantation. The number and size of sporophores increase with increasing nutrient concentration in the solid culture medium up to a level of 7% malt extract. Larger containers plugged with cotton are more favorable for the production of sporophores of normal size than smaller, hermetically closed containers.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus

2021 ◽  
Vol 2 (2) ◽  
pp. 538-553
Author(s):  
Natacha Coelho ◽  
Alexandra Filipe ◽  
Bruno Medronho ◽  
Solange Magalhães ◽  
Carla Vitorino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Average Pore Size ◽  
Physiological Responses ◽  
Gum Arabic ◽  
Shoot Elongation ◽  
Hydroxyethyl Cellulose ◽  
Plant Culture

In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.

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