In Vitro Study of Tooth Surfaces After Interproximal Enamel Reduction: Extraoral Scanner and SEM Analysis

Objective: Preventive procedures using pit and fissure sealants are one of the important aspects of pediatric dental practice. The objectives of this in vitro study were to comparatively evaluate microleakage and resin tag penetration of a flowable resin used as a sealant on molars after preparation with Conventional, Enameloplasty and Fissurotomy techniques. Method: A total of 48 teeth were divided into 3 groups (n-16). Group A(CST),Group B (EST),and Group C (FT). Following the fissure preparation sealant was applied as per manufacturer's instructions. 8 samples in each group were used for Microleakage and Resin tag analysis. Microleakage analysis was done under Stereomicroscope after Methylene blue dye immersion by using scoring system. The resin tag analysis was done by measuring the length of resin tags on scanning electron microscope (SEM) images consisting of 10μm scale taken under optical zoom of 2000 X. Results: Significantly lesser microleakage score (P<0.01) and longer resin tag penetration (P<0.001) observed in EST(mean score 0.5±0.53) & (12.19±1.93μm) when compared to CST(mean score 1.75±0.89) & (5.96±1.84μm)and FT (mean score 1.5±0.53) & (6.76±1.82μm) which showed more microleakage and short resin tags. No statistical significance between CST and FT was found. Conclusion: EST has proven to be an excellent method for preparation of pits and fissures when Flowable composite is used as a sealant because of its lesser microleakage and longer resin tag penetration.

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Enamel Apperance after Orthodontic Attachment Removal. In vitro SEM analysis

Revista de Chimie ◽

10.37358/rc.17.1.5403 ◽

2017 ◽

Vol 68 (1) ◽

pp. 125-127

Author(s):

Alexandrina Muntean ◽

Anca Stefania Mesaros ◽

Anca Porumb ◽

Stanca Cuc ◽

Marioara Moldovan ◽

...

Keyword(s):

Artificial Saliva ◽

In Vitro Study ◽

Sem Analysis ◽

Point Of View ◽

Vitro Study ◽

Enamel Surface ◽

Surface Appearance ◽

Adhesive Remnant Index ◽

Materials Used

The aim of this in vitro study was to assess the enamel surface appearance and Adhesive Remnant Index (ARI) after braces removal. On teeth vestibular surfaces 90 braces were bonded as follows: metallic, ceramics respectively sapphire using as bonding material Opal Seal� (Ultradent, SUA). Teeth were divided in 3 groups (N=30) and stored for 3 months in artificial saliva, tea and Coca-Cola�. For teeth stored in tea and Coca Cola�, ARI express lower values, indicating a risk for enamel injury as fracture occurs at adhesive-enamel interface. From practical point of view, this in vitro study enhance that braces detachment, finishing and polishing are risk manoeuvres for enamel surface. Materials used for collage must present specific properties in order to reduce enamel fracture.

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Modulatory effects by neodymium-doped yttrium aluminum garnet laser on fibroblast attachment to single rooted tooth surfaces following ultrasonic scaling and root planning: An in vitro study

Journal of Indian Society of Periodontology ◽

10.4103/0972-124x.145819 ◽

2015 ◽

Vol 19 (1) ◽

pp. 25 ◽

Cited By ~ 3

Author(s):

KiranKumar Ganji ◽

Sunil Pendor ◽

Shanta Negi ◽

Malathi Krishnamurthy

Keyword(s):

Yttrium Aluminum Garnet ◽

In Vitro Study ◽

Vitro Study ◽

Yttrium Aluminum Garnet Laser ◽

Yttrium Aluminum ◽

Tooth Surfaces ◽

Fibroblast Attachment ◽

Root Planning ◽

Scaling And Root Planning

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Effect of Vital Bleaching on Micromorphology of Enamel Surface: an in Vitro Study

Primary Dental Journal ◽

10.1177/2050168420980966 ◽

2021 ◽

Vol 10 (1) ◽

pp. 126-131

Author(s):

Zakaria Karimi ◽

Houda Saoui ◽

Majid Sakout ◽

Faiza Abdallaoui

Keyword(s):

In Vitro Study ◽

Sem Analysis ◽

Vitro Study ◽

Enamel Surface ◽

The Other ◽

Bleaching Agent ◽

Surface Porosity ◽

Scanning Electron ◽

At Home

Objectives: The aims of this in vitro study was to investigate the effects of bleaching agents commonly used in micromorphology of the enamel surface and to assess the effect of concentration and of adding fluoride in the bleaching agents. Methods: Sixty freshly extracted intact teeth were stored in distilled water. One half of each tooth was served as control, the other part was treated with bleaching agent. Samples were randomly divided into six groups of ten, according to the bleaching agents: G1- at-home-CP10; G2- at-home-CP16; G3- at-home-CP22; G4- in-office-CP35; G5- in-office-HP40 with fluoride; G6- in-office-HP40 without fluoride. Enamel specimens for each group were then submitted to a quantitative scanning electron microscopy. Number of pores and their diameter were measured to assess porosity of enamel surface. Results: SEM analysis revealed enamel surface porosity after bleaching. Significant increase in number and major diameter of pores in bleached samples (p<0.001) were observed. The comparison between samples treated with 10% PC and samples treated with 22% PC showed significant increase in number of pores (p=0.006) and major diameter (p=0.001) from samples treated with 22% PC. Statistical analyses showed significant increase in the number of pores (p=0.006) from samples treated with 40% HP without fluoride compared to samples treated with 40% HP containing fluoride. Conclusions: Bleaching products with low concentration cause less porosity at surface of the enamel compared to concentrated products. Adding fluoride in the bleaching agent appears to reduce porosity of enamel surface.

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