Enamel Apperance after Orthodontic Attachment Removal. In vitro SEM analysis

2017 ◽  
Vol 68 (1) ◽  
pp. 125-127
Author(s):  
Alexandrina Muntean ◽  
Anca Stefania Mesaros ◽  
Anca Porumb ◽  
Stanca Cuc ◽  
Marioara Moldovan ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Point Of View ◽  
Vitro Study ◽  
Enamel Surface ◽  
Surface Appearance ◽  
Adhesive Remnant Index ◽  
Materials Used

The aim of this in vitro study was to assess the enamel surface appearance and Adhesive Remnant Index (ARI) after braces removal. On teeth vestibular surfaces 90 braces were bonded as follows: metallic, ceramics respectively sapphire using as bonding material Opal Seal� (Ultradent, SUA). Teeth were divided in 3 groups (N=30) and stored for 3 months in artificial saliva, tea and Coca-Cola�. For teeth stored in tea and Coca Cola�, ARI express lower values, indicating a risk for enamel injury as fracture occurs at adhesive-enamel interface. From practical point of view, this in vitro study enhance that braces detachment, finishing and polishing are risk manoeuvres for enamel surface. Materials used for collage must present specific properties in order to reduce enamel fracture.

scholarly journals Effects of Composite Resin on the Enamel after Debonding: An In Vitro Study—Metal Brackets vs. Ceramic Brackets

2021 ◽  
Vol 11 (16) ◽  
pp. 7353
Author(s):  
Alexandru Vlasa ◽  
Eugen Silviu Bud ◽  
Mariana Păcurar ◽  
Luminița Lazăr ◽  
Laura Streiche ◽  
...  
Keyword(s):  
Composite Resin ◽  
In Vitro Study ◽  
Visual Observation ◽  
Vitro Study ◽  
Enamel Surface ◽  
Prismatic Structure ◽  
Adhesive Remnant Index ◽  
Ceramic Brackets ◽  
Bonding Technique

Fixed orthodontic therapies include several procedures that can affect the enamel surface. The aim of this study was to assess the action of composite resin on the surface of the tooth through variation of enamel changes after debonding metal and ceramic brackets, by means of scanning electron microscopy. An in vitro study was conducted on 48 human premolar specimens, which were extracted within a period of two months for orthodontic purposes. On half of them, metal brackets were bonded, and on the other half, ceramic brackets (Al2O3) were bonded, using light cure adhesive paste and a two-step, etch-and-bonding technique. The brackets were debonded after 24 h using a straight debonding plier. The adhesive remnant index (ARI) was determined by visual observation of the specimen. Post-debonding scans were aligned with the baseline, and the surfaces’ changes were quantified. A quantitative analysis was made on the debonded brackets to determine the presence or absence of enamel on the base pad. Evaluation of pre-bonded and post-clean-up enamel surface revealed no crack and increased roughness in both ceramic and metal brackets, which was higher for the ceramic ones. The enameled band (perikymata), artificial caries, or the superficial fissures revealed in the pretreatment stage were replaced with the loss of the prismatic structure and the presence of remnant adhesive. No enamel substance was found on the base pad. The ARI tooth was higher for metallic brackets than for ceramic ones. Metallic brackets and ceramic brackets have undergone mechanical changes by showing fractures in their structure. According to our present investigation, we can conclude that the adhesive composite resin is safe for use on both metal and ceramic brackets in orthodontic treatments, with no iatrogenic enamel damages.

Effect of Vital Bleaching on Micromorphology of Enamel Surface: an in Vitro Study

2021 ◽  
Vol 10 (1) ◽  
pp. 126-131
Author(s):  
Zakaria Karimi ◽  
Houda Saoui ◽  
Majid Sakout ◽  
Faiza Abdallaoui
Keyword(s):  
In Vitro Study ◽  
Sem Analysis ◽  
Vitro Study ◽  
Enamel Surface ◽  
The Other ◽  
Bleaching Agent ◽  
Surface Porosity ◽  
Scanning Electron ◽  
At Home

Objectives: The aims of this in vitro study was to investigate the effects of bleaching agents commonly used in micromorphology of the enamel surface and to assess the effect of concentration and of adding fluoride in the bleaching agents. Methods: Sixty freshly extracted intact teeth were stored in distilled water. One half of each tooth was served as control, the other part was treated with bleaching agent. Samples were randomly divided into six groups of ten, according to the bleaching agents: G1- at-home-CP10; G2- at-home-CP16; G3- at-home-CP22; G4- in-office-CP35; G5- in-office-HP40 with fluoride; G6- in-office-HP40 without fluoride. Enamel specimens for each group were then submitted to a quantitative scanning electron microscopy. Number of pores and their diameter were measured to assess porosity of enamel surface. Results: SEM analysis revealed enamel surface porosity after bleaching. Significant increase in number and major diameter of pores in bleached samples (p<0.001) were observed. The comparison between samples treated with 10% PC and samples treated with 22% PC showed significant increase in number of pores (p=0.006) and major diameter (p=0.001) from samples treated with 22% PC. Statistical analyses showed significant increase in the number of pores (p=0.006) from samples treated with 40% HP without fluoride compared to samples treated with 40% HP containing fluoride. Conclusions: Bleaching products with low concentration cause less porosity at surface of the enamel compared to concentrated products. Adding fluoride in the bleaching agent appears to reduce porosity of enamel surface.

scholarly journals Physicochemical Characteristics of Arginine Enriched NaF Varnish: An In Vitro Study

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2998
Author(s):  
Mohammed Nadeem Bijle ◽  
Manikandan Ekambaram ◽  
Edward Lo ◽  
Cynthia Yiu
Keyword(s):  
Molecular Dynamics ◽  
Physical Properties ◽  
Artificial Saliva ◽  
Chemical Properties ◽  
In Vitro Study ◽  
Physicochemical Characteristics ◽  
Vitro Study ◽  
Physical And Chemical ◽  
Stable Matrix

The in vitro study objectives were to investigate the effect of arginine (Arg) incorporation in a 5% sodium fluoride (NaF) varnish on its physical and chemical properties including F/Arg release. Six experimental formulations were prepared with L-arginine (L-Arg) and L-arginine monohydrochloride at 2%, 4%, and 8% w/v in a 5% NaF varnish, which served as a control. The varnishes were subjected to assessments for adhesion, viscosity, and NaF extraction. Molecular dynamics were simulated to identify post-dynamics total energy for NaF=Arg/Arg>NaF/Arg<NaF concentrations. The Arg/F varnish release profiles were determined in polyacrylic lactate buffer (pH-4.5; 7 days) and artificial saliva (pH-7; 1 h, 24 h, and 12 weeks). Incorporation of L-Arg in NaF varnish significantly influences physical properties ameliorating retention (p < 0.001). L-Arg in NaF varnish institutes the Arg-F complex. Molecular dynamics suggests that NaF>Arg concentration denotes the stabilized environment compared to NaF<Arg (p < 0.001). The 2% Arg-NaF exhibits periodic perennial Arg/F release and shows significantly higher integrated mean F release than NaF (p < 0.001). Incorporating 2% L-arginine in 5% NaF varnish improves its physical properties and renders a stable matrix with enduring higher F/Arg release than control.

scholarly journals Effect of topical fluoride on microshear bond strength of primary enamel to composite, microhardness of enamel and its surface morphology: An in vitro study

2019 ◽  
Vol 13 (4) ◽  
pp. 305-310
Author(s):  
Mina Biria ◽  
Sajedeh Namaei Ghasemi ◽  
Seyedeh Mahsa Sheikh-Al-Eslamian ◽  
Narges Panahandeh
Keyword(s):  
Bond Strength ◽  
Artificial Saliva ◽  
Morphological Characteristics ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Modes Of Failure ◽  
Group 3 ◽  
Microshear Bond Strength

Background. This in vitro study aimed to evaluate the microshear bond strength (μSBS), microhardness and morphological characteristics of primary enamel after treating with sodium fluoride (NaF) and acidulated phosphate fluoride (APF). Methods. Forty-eight primary canines were cut into mesial and distal sections and assigned to five groups randomly: group 1 (immersed in saliva as a control), group 2 (treated with NAF and immersed in saliva for 30 minutes), group 3 (treated with APF and immersed in saliva for 30 minutes), group 4 (treated with NAF and immersed in saliva for 10 days), and group 5 (treated with APF and immersed in saliva for 10 days). Composite resin (Filtek Z250) was bonded on the specimens (n=15) for measuring the μSBS. After storage in 37°C artificial saliva for 24 hours, µSBS and Vickers hardness tests (10 readings) were performed. The data were analyzed using one-way ANOVA and Kolmogorov-Smirnov, Levene’s and Tukey HSD tests (P<0.05). Morphological analysis of enamel and modes of failure were carried out under a scanning electron microscope (SEM) on two remaining specimens. Results. Significant differences in μSBS were only noted between groups 2 and 4 (P=0.024). Group 3 showed a significant decrease in hardness after storage in artificial saliva (P<0.001), with a significantly lower hardness than the other groups (P<0.001). The SEM observations showed irregular particles in groups 3 and 5; uniform, smooth and thin coats were seen in groups 2 and 4. Conclusion. Fluoride therapy with NaF and APF gels prior to restorative treatments had no adverse effects on the microshear bond strength.

scholarly journals An In Vitro Study of the Effect of Fluoridated Milk on Oral Bacterial Biofilms

2000 ◽  
Vol 66 (4) ◽  
pp. 1720-1723 ◽  
Author(s):  
J. Pratten ◽  
R. Bedi ◽  
M. Wilson
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bacterial Biofilms ◽  
Dental Plaques

ABSTRACT Microcosmic dental plaques were grown in artificial saliva and supplemented with either milk or fluoridated milk. The presence of fluoride in the milk increased the pH of the biofilms and reduced the proportions of streptococci, demonstrating that in this model, fluoridation of milk produces biofilms with reduced cariogenic potential.

scholarly journals Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2458 ◽  
Author(s):  
Bruna Raquel Zancopé ◽  
Vanessa B. Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte ◽  
Vanessa Pardi ◽  
...  
Keyword(s):  
Contact Angle ◽  
Biofilm Formation ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Dry Weight ◽  
Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Biofilm Development

BackgroundA carbon dioxide (CO2) laser has been used to morphologically and chemically modify the dental enamel surface as well as to make it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of a CO2laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2laser (λ = 10.6 µM).MethodsFor this in vitro study, 96 specimens of bovine enamel were employed, which were divided into two groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by one, three and five days under intermittent cariogenic condition in the irradiated and non-irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and, in fifth day, were evaluated by polysaccharides analysis, quantitative real time Polymerase Chain Reaction (PCR) as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test.ResultsThe mean of log CFU/mL obtained for the one-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to the CO2laser, CFU/mL and CFU/dry weight in three day was reduced significantly compared with control group. The difference in the genes expression (Glucosyltransferases (gtfB) and Glucan-binding protein (gbpB)) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with the CO2laser. Similar morphology was also visible with both treatments; however, the irradiated group revealed evidence of melting and fusion in the specimens.ConclusionIn conclusion, CO2laser irradiation modifies the energy surface and disrupts the initial biofilm formation.

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