scholarly journals Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids

2017 ◽  
Vol 52 (9) ◽  
pp. 814-817 ◽  
Author(s):  
Fernanda Motta da Costa Santos ◽  
Giovana Augusta Torres ◽  
Vânia Helena Techio ◽  
Antônio Vander Pereira ◽  
Lisete Chamma Davide
Keyword(s):  
In Situ Hybridization ◽  
Pearl Millet ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization ◽  
Elephant Grass ◽  
Nick Translation ◽  
Flower Buds ◽  
Genomic Probe ◽  
Chromosomal Pairing

Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.

Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids

1997 ◽  
Vol 95 (8) ◽  
pp. 1320-1324 ◽  
Author(s):  
R. J. Snowdon ◽  
W. Köhler ◽  
W. Friedt ◽  
A. Köhler
Keyword(s):  
In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization

The genome composition of hexaploid Psammopyrum athericum and octoploid Psammopyrum pungens (Poaceae: Triticeae)

Genome ◽  
2003 ◽  
Vol 46 (1) ◽  
pp. 164-169 ◽  
Author(s):  
Pernilla Ellneskog-Staam ◽  
Björn Salomon ◽  
Roland von Bothmer ◽  
Kesara Anamthawat-Jónsson
Keyword(s):  
In Situ Hybridization ◽  
Genome Analysis ◽  
Genomic In Situ Hybridization ◽  
Agropyron Cristatum ◽  
Genome Composition ◽  
Genomic Constitution ◽  
Hybridization Pattern ◽  
Genomic Probe

The genomic constitution of two species in the genus Psammopyrum, i.e., Ps. athericum (2n = 6x = 42) and Ps. pungens (2n = 8x = 56), was studied by genomic in situ hybridization (GISH). In Ps. athericum, one diploid chromosome set hybridized to a genomic probe from Pseudoroegneria ferganensis (St genome), one diploid set to a probe from Agropyron cristatum (P genome), and one diploid set to a probe from Thinopyrum junceiforme (EbEe genomes) or Th. bessarabicum (Eb genome). Substituting the St-genome probe with an L-genome probe from Festucopsis serpentinii resulted in exactly the same hybridization pattern, suggesting a genomic constitution of EStP or ELP for Ps. athericum. The same probes used on Ps. pungens showed two diploid sets of chromosomes hybridizing to the St-genome probe, one diploid set hybridizing to the P-genome probe, and one diploid set hybridizing to the EbEe-genome probe. The L-genome probe hybridized to approximately 14 of the chromosomes that were labeled by the St-genome probe. Hence the genomic constitution for Ps. pungens is proposed to be EStStP or EStLP.Key Words: Psammopyrum athericum, Psammopyrum pungens, in situ hybridization, Elytrigia pycnantha, Elytrigia pungens, genome analysis.

Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 469-472 ◽  
Author(s):  
A Benabdelmouna ◽  
G Guéritaine ◽  
M Abirached-Darmency ◽  
H Darmency
Keyword(s):  
In Situ Hybridization ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Interspecific Hybrid ◽  
Interspecific Hybrids ◽  
Genomic In Situ Hybridization ◽  
Interspecific Crosses ◽  
Wild Radish ◽  
Raphanus Raphanistrum

Genomic in situ hybridization (GISH) applied to the F1 interspecific hybrid between oilseed rape (Brassica napus, AACC, 2n = 38) and wild radish (Raphanus raphanistrum, RrRr, 2n = 18) showed the predicted 19 chromosomes from B. napus and 9 chromosomes from R. raphanistrum. The very low female fertility of these interspecific hybrids when backcrossed to R. raphanistrum led to only two descendants. Their chromosome number varied between 45 and 48. Both of these progenies showed only 9 chromosomes from R. raphanistrum and 36–39 chromosomes from B. napus. These results indicate the efficiency and limits of GISH as a suitable tool to assess and interpret the behavior of chromosomes after such interspecific crosses. The unexpected chromosome combination is discussed.Key words: genomic in situ hybridization, interspecific hybrid, introgression, oilseed rape, wild radish.

scholarly journals Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH)

2019 ◽  
Vol 42 (4) ◽  
Author(s):  
Rosilda Cintra de Souza ◽  
Daniela de Argollo Marques ◽  
Marcel Mamede de Carvalho Filho ◽  
Ana Rafaela da Silva Oliveira ◽  
Walter José Siqueira ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Interspecific Hybrids ◽  
Pollen Viability ◽  
Genomic In Situ Hybridization ◽  
Genome Composition
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