scholarly journals Análise de restrição de DNA ribossomal amplificado (ARDRA) pode diferenciar Fusarium solani f. sp. phaseoli de F. solani f. sp. glycines

2002 ◽  
Vol 27 (6) ◽  
pp. 631-634 ◽  
Author(s):  
VIRGÍNIA C. de OLIVEIRA ◽  
JEFFERSON L. S. da COSTA
Keyword(s):  
Glycine Max ◽  
Phaseolus Vulgaris ◽  
Ribosomal Dna ◽  
Fusarium Solani ◽  
Restriction Analysis ◽  
Fusarium Spp ◽  
Formae Speciales ◽  
Rdna Its2 ◽  
Dna Restriction ◽  
Para Determinar

Métodos moleculares têm sido utilizados para caracterizar a diversidade entre isolados de Fusarium spp. patogênicos e não patogênicos a uma cultura e, para determinar relações genéticas entre formae speciales. Testes de patogenicidade realizados em soja (Glycine max) e feijoeiro (Phaseolus vulgaris) com 17 isolados de Fusarium solani não demonstraram especificidade de hospedeiros. Utilizou-se a técnica ARDRA (Amplified Ribosomal DNA Restriction Analysis) para analisar a região ITS1 - 5,8S rDNA - ITS2, amplificada com os primers ITS5 e ITS4. Os produtos amplificados foram digeridos com as enzimas de restrição Hae III e Msp I. Os padrões de bandas gerados pela digestão com a enzima Hae III permitiram diferenciar três grupos entre os isolados de F. solani, sendo um grupo específico para isolados de F. solani f. sp. phaseoli com 100% de similaridade entre os 11 isolados. Entre os isolados de F. solani f. sp glycines foram observados dois padrões distintos de restrição. A técnica de ARDRA utilizando a enzima Hae III apresenta, portanto, potencial para utilização como um marcador para diferenciação entre as formae specialesphaseoli e glycines, dentro do complexo F. solani.

Photosynthetic Bradyrhizobia from Aeschynomene spp. Are Specific to Stem-Nodulated Species and Form a Separate 16S Ribosomal DNA Restriction Fragment Length Polymorphism Group

1999 ◽  
Vol 65 (7) ◽  
pp. 3084-3094 ◽  
Author(s):  
Flore Molouba ◽  
Jean Lorquin ◽  
Anne Willems ◽  
Bart Hoste ◽  
Eric Giraud ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Rhodobacter Capsulatus ◽  
Restriction Analysis ◽  
Dna Amplification ◽  
Rrna Gene ◽  
Gene Probe ◽  
16S Ribosomal Dna ◽  
Photosynthetic Genes ◽  
Dna Restriction ◽  
Layer Chromatography

ABSTRACT We obtained nine bacterial isolates from root or collar nodules of the non-stem-nodulated Aeschynomene species A. elaphroxylon, A. uniflora, or A. schimperi and 69 root or stem nodule isolates from the stem-nodulated Aeschynomene species A. afraspera, A. ciliata, A. indica,A. nilotica, A. sensitiva, and A. tambacoundensis from various places in Senegal. These isolates, together with 45 previous isolates from variousAeschynomene species, were studied for host-specific nodulation within the genus Aeschynomene, also revisiting cross-inoculation groups described previously by D. Alazard (Appl. Environ. Microbiol. 50:732–734, 1985). The whole collection ofAeschynomene nodule isolates was screened for synthesis of photosynthetic pigments by spectrometry, high-pressure liquid chromatography, and thin-layer chromatography analyses. The presence ofpuf genes in photosyntheticAeschynomene isolates was evidenced both by Southern hybridization with a Rhodobacter capsulatus photosynthetic gene probe and by DNA amplification with primers defined from photosynthetic genes. In addition, amplified 16S ribosomal DNA restriction analysis was performed on 45 Aeschynomeneisolates, including strain BTAi1, and 19 reference strains fromBradyrhizobium japonicum, Bradyrhizobium elkanii, and other Bradyrhizobium sp. strains of uncertain taxonomic positions. The 16S rRNA gene sequence of the photosynthetic strain ORS278 (LMG 12187) was determined and compared to sequences from databases. Our main conclusion is that photosynthetic Aeschynomene nodule isolates share the ability to nodulate particular stem-nodulated species and form a separate subbranch on the Bradyrhizobium rRNA lineage, distinct from B. japonicum and B. elkanii.

scholarly journals Evaluation of amplified ribosomal DNA restriction analysis as a method for the identification of Botryosphaeria species

2005 ◽  
Vol 245 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Artur Alves ◽  
Alan J.L. Phillips ◽  
Isabel Henriques ◽  
António Correia
Keyword(s):  
Ribosomal Dna ◽  
Restriction Analysis ◽  
Dna Restriction

scholarly journals Identification of NDM-1 in a Putatively Novel Acinetobacter Species (“NB14”) Closely Related to Acinetobacter pittii

2015 ◽  
Vol 59 (10) ◽  
pp. 6657-6660 ◽  
Author(s):  
Paula Espinal ◽  
Noraida Mosqueda ◽  
Murat Telli ◽  
Tanny van der Reijden ◽  
Dora Rolo ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Restriction Analysis ◽  
Conjugative Plasmid ◽  
Acinetobacter Species ◽  
Content Type ◽  
16S Ribosomal Dna ◽  
Acinetobacter Pittii ◽  
Dna Restriction ◽  
Molecular Sequencing

ABSTRACTIn this study, we describe the molecular characterization of a plasmid-locatedblaNDM-1harbored by anAcinetobacterclinical isolate recovered from a patient in Turkey that putatively constitutes a novelAcinetobacterspecies, as shown by its distinct ARDRA (amplified 16S ribosomal DNA restriction analysis) profile and molecular sequencing techniques.blaNDM-1was carried by a conjugative plasmid widespread among non-baumannii Acinetobacterisolates, suggesting its potential for dissemination before reaching more clinically relevantAcinetobacterspecies.

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