scholarly journals Efficacy of a gE-deleted, bovine herpesvirus 1 (BoHV-1) inactivated vaccine

2009 ◽  
Vol 29 (7) ◽  
pp. 545-551 ◽  
Author(s):  
Alessandra D. Silva ◽  
Paulo A. Esteves ◽  
Diogenes Dezen ◽  
Anna P. Oliveira ◽  
Fernando R. Spilki ◽  
...  

Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of economic losses in cattle. Vaccination has been widely applied to minimize losses induced by BoHV-1 infections. We have previously reported the development of a differential BoHV-1 vaccine, based on a recombinant glycoprotein E (gE)-deleted virus (265gE-). In present paper the efficacy of such recombinant was evaluated as an inactivated vaccine. Five BoHV-1 seronegative calves were vaccinated intramuscularly on day 0 and boostered 30 days later with an inactivated, oil adjuvanted vaccine containing an antigenic mass equivalent to 10(7.0) fifty per cent cell culture infectious doses (CCID50) of 265gE-. Three calves were kept as non vaccinated controls. On day 60 post vaccination both vaccinated and controls were challenged with the virulent parental strain. No clinical signs or adverse effects were seen after or during vaccination. After challenge, 2/5 vaccinated calves showed mild clinical signs of infection, whereas all non vaccinated controls displayed intense rhinotracheitis and shed virus for longer and to higher titres than vaccinated calves. Serological responses were detected in all vaccinated animals after the second dose of vaccine, but not on control calves. Following corticosteroid administration in attempting to induce reactivation of the latent infection, no clinical signs were observed in vaccinated calves, whereas non vaccinated controls showed clinical signs of respiratory disease. In view of its immunogenicity and protective effect upon challenge with a virulent BoHV-1, the oil adjuvanted preparation with the inactivated 265gE- recombinant was shown to be suitable for use as a vaccine.

2008 ◽  
Vol 1 (1) ◽  
pp. 18-31
Author(s):  
Sava Lazić ◽  
Tamaš Petrović ◽  
Diana Lupulović ◽  
Dejan Bugarski ◽  
Ivan Pušić ◽  
...  

Bovine herpesvirus type 1 infection (IBR/IPV virus) presents the most spread infection in nowadays cattle breeding. Bovine herpesvirus-1 (BHV-1) may be an agent of serious health disorders causing great economic losses. Since the largest number of cattle in Southern Baåka and Srem district are raised in small herds (what may considerably influence effective implementation of program for control and eradication of BHV-1 infection) it was necessary to investigate the prevalence of BHV-1 infection in the cattle population in this area, what was the aim of this work. Detection and estimation of the presence and prevalence of BHV-1 was done by detection of specific antibodies against BHV-1 in sera samples of cattle raised individually or in small herds (up to 20 animals) collected during the State Program of Disease Control in 2005 and 2006. The sampling was randomly carried out taking care that the samples from all the settlements and municipalities in Southern Bačka and Srem were present. A total of 16,610 samples was collected. Animals seropositive for BHV-1 were detected in all municipalities, but not in all settlements. The highest percent of seropositive animals was found in Beočin municipality(27.27%), Titel municipality (27.16%), Žabalj (22.45%) and Stara Pazova municipality (22.15%). The lowest prevalence was in Bački Petrovac (8.16%) and Temerin municipalities (9.68%). In other municipalities the prevalence ranged between 10% and 20%. From the obtained results it can be concluded that BHV-1 infection was not present in 10 settlements in Southern Bačka and 14 settlements in Srem district. In other settlements (151) the percentage of seropositive animals ranged from 1 to 50%. However, if we analyze the obtained results in the area, it may be concluded that the prevalence is almost the same: in Srem district there were 18.42% and in Southern Bačka 18.79% seropositive animals. Low prevalence was detected in the majority of settlements. The data that in 24 settlements in both districts there were no seropositive animals His an encouraging information. This points on a possibility of easier and faster implementation of control measures and eradication of BHV-1 infection. The results obtained in serology examination provide us an insight in immunology status of small herds in Southern Bačka and Srem districts, what is a starting point in designing and implementing necessary measures with the aim to prevent cases of this disease, as well as its spreading and eradication.


2009 ◽  
Vol 15 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Mário CS Brum ◽  
Charles Coats ◽  
Rajkumari B Sangena ◽  
Allan Doster ◽  
Clinton Jones ◽  
...  

Vaccine ◽  
2001 ◽  
Vol 19 (32) ◽  
pp. 4795-4804 ◽  
Author(s):  
Mylène Lemaire ◽  
Frédéric Schynts ◽  
Gilles Meyer ◽  
Jean-Pierre Georgin ◽  
Eric Baranowski ◽  
...  

Vaccine ◽  
1994 ◽  
Vol 12 (5) ◽  
pp. 439-444 ◽  
Author(s):  
M.J. Kaashoek ◽  
A. Moerman ◽  
J. Madić ◽  
F.A.M. Rijsewijk ◽  
J. Quak ◽  
...  

1996 ◽  
Vol 45 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Xavier Rebordosa ◽  
Jaume Piñol ◽  
Josep A. Pérez-Pons ◽  
Jorge Lloberas ◽  
Jordi Naval ◽  
...  

2016 ◽  
Vol 51 (5) ◽  
pp. 676-679 ◽  
Author(s):  
Andre Penido Oliveira ◽  
Marcos Bryan Heinemann ◽  
Adriana Cortez ◽  
Paula Maria Pires do Nascimento ◽  
Romulo Cerqueira Leite ◽  
...  

Abstract: The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV-1) in follicular fluid and in cumulus-oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV-1 by COC or follicular fluid.


2003 ◽  
Vol 153 (7) ◽  
pp. 209-212 ◽  
Author(s):  
M. Dispas ◽  
F. Schynts ◽  
M. Lemaire ◽  
C. Letellier ◽  
E. Vanopdenbosch ◽  
...  

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