scholarly journals Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

2010 ◽  
Vol 30 (11) ◽  
pp. 918-920 ◽  
Author(s):  
Priscilla F Gerber ◽  
Flávia F Pinto ◽  
Marcos B Heinemann ◽  
Zélia I.P Lobato
Keyword(s):  
Real Time ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Polymerase Chain

The dynamics of porcine circovirus type 2 (PCV2) shedding in semen of naturally infected boars was studied. Semen was collected serially each 15 or 20 days during 62 days from 5 boars from a herd and from 11 boars from an artificial insemination center. All boars were positive for PCV2 DNA by nested polymerase chain reaction of raw semen in at least two sampling dates, and most of them had detectable shedding in all sampling dates. Real-time quantitative PCR was performed in 23 samples. All samples showed low amounts of PCV2 DNA, ranging from 98 to 652 PCV2 copies/mL. No differences between the frequencies of PCV2 DNA shed in semen were found considering herds and age of boars. PCV2 shedding in the semen can occur continuously or intermittently up to 60 days in naturally infected boars at 12 to 42 months old in absence of PCV2 clinical signs. These results demonstrate sporadic and long-term shedding patterns of low amounts of PCV2 DNA in semen from naturally infected boars.

scholarly journals Diagnosis of post-weaning multisystemic wasting syndrome in pigs in Brazil caused by porcine circovirus type 2

2003 ◽  
Vol 55 (5) ◽  
pp. 522-527 ◽  
Author(s):  
J.R. Ciacci-Zanella ◽  
N. Morés
Keyword(s):  
Clinical Signs ◽  
Rflp Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Nested Polymerase Chain Reaction ◽  
Wasting Syndrome ◽  
Thymus Atrophy ◽  
Granulomatous Lymphadenitis

This report describes the first preliminary characterization of porcine circovirus type 2 (PCV2) isolates from pigs affected with post-weaning multisystemic wasting syndrome (PMWS) in Brazil. Diseased pigs were examined at necropsy and by histopathology. Macroscopic and microscopic analyses revealed lesions reported to be typical of PMWS, which included, respectively, emaciation, enlargement of lymph nodes, thymus atrophy and interstitial pneumonia, and granulomatous lymphadenitis with syncytial cells, among others. Using nested polymerase chain reaction (PCR) or imunoperoxidase it was possible to detected DNA or antigen of PCV2, respectively. The PCR' s amplified fragment could be differentiated from PCV1 and PCV2 from one another by restriction fragment length polymorphism (RFLP) analysis. PCV2 DNA was detected in 70% (14/20) of samples of pigs with clinical signs and lesions associated with PMWS. This study shows that PCV2 is associated with lesions and symptoms indicative of PMWS in pigs. It is also shown that the Brazilian PCV2 isolates may have variation in their genome.

scholarly journals Granulomatous Necrotizing Myositis in Swine Affected by Porcine Circovirus Disease

2017 ◽  
Vol 55 (2) ◽  
pp. 268-272 ◽  
Author(s):  
Guilherme Konradt ◽  
Raquel A. S. Cruz ◽  
Daniele M. Bassuino ◽  
Matheus V. Bianchi ◽  
Caroline P. de Andrade ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Clinical Signs ◽  
Giant Cells ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Lower Amount ◽  
Clinical Syndromes ◽  
Necrotizing Myositis ◽  
Polymerase Chain

Porcine circovirus type 2 (PCV2) is associated with multiple clinical syndromes in pigs, known as porcine circovirus diseases. This work describes an outbreak of porcine circovirus diseases with severe lesions affecting the skeletal muscle. Ninety-two pigs had apathy, weight loss, and diarrhea over a clinical course of 7 to 10 days. Approximately 30 of the pigs had stiff gait, muscle weakness, hind limb paresis, and recumbency. Twelve of the 92 pigs were necropsied, and 4 had pale discoloration of skeletal muscles with microscopic lesions of granulomatous necrotizing myositis. Immunohistochemistry of skeletal muscle showed that PCV2 antigen was located primarily in the cytoplasm and nuclei of macrophages, lymphocytes, and multinucleated giant cells, with a lower amount in the cytoplasm of endothelial cells, necrotic fibers, and satellite cells. Affected muscle samples were polymerase chain reaction–positive for PCV2 and the amplicon exhibited 99% identity with sequences belonging to the PCV2b genotype. Locomotor clinical signs and granulomatous necrotizing myositis should be considered as another expression of PCV2 infection in pigs.

scholarly journals Survey Of Infectious Agents Associated With Porcine Respiratory Disease Complex (PRDC) In Serbian Swine Herds Using Polymerase Chain Reaction (PCR) Detection

2015 ◽  
Vol 65 (1) ◽  
pp. 79-88 ◽  
Author(s):  
SAVIĆ Božidar ◽  
RADANOVIĆ Oliver ◽  
JOVIČIĆ Dubravka ◽  
NEŠIĆ Ksenija ◽  
IVANOVIĆ Snežana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Disease ◽  
Respiratory Virus ◽  
Mycoplasma Hyopneumoniae ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Porcine Respiratory

Abstract A retrospective study on 235 natural cases of Porcine Respiratory Disease Complex in order to determine the etiological agents, their prevalence and interrelationships was performed in Serbia. Lung tissue samples were analyzed by Polymerase Chain Reaction for the presence of Porcine circovirus type 2, Porcine reproductive and respiratory virus, Swine influenza virus, Mycoplasma hyopneumoniae, Pasteurella multocida, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Streptococcus suis and Arcanobacterium pyogenes. A total of 49 different combinations of viral and bacterial pathogens were found. Five different viral and viral/Mhp co-infections were detected. Monobacterial infections were found in 150 cases and polybacterial infection was detected in 85 samples. PCV2 was the main virus detected, and Pm was the most aggressive secondary pathogen detected in PRDC. The reason for PRDC being so prevalent among Serbian pigs is most likely due to the large number of risk factors in the conventional farrow-to-finish system, compared to multi-site production systems. Therefore, measures aimed at a better control of respiratory viruses, particularly Porcine circovirus type 2 and Porcine reproductive and respiratory virus, as well as Mycoplasma hyopneumoniae infections, and adoption of rational decisions on respiratory bacterial pathogens specific therapeutic and preventive strategies at herd level, simultaneously with significant improvements on farm management should reduce the occurrence of PRDC.

scholarly journals Detection of Porcine Circovirus Type 2 in Feces of Pigs with or without Enteric Disease by Polymerase Chain Reaction

2003 ◽  
Vol 15 (4) ◽  
pp. 369-373 ◽  
Author(s):  
Jeong S. Yang ◽  
Dae S. Song ◽  
So Y. Kim ◽  
Kwang S. Lyoo ◽  
Bong K. Park
Keyword(s):  
Polymerase Chain Reaction ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Enteric Disease ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Fecal Samples ◽  
Polymerase Chain

To establish the sensitive polymerase chain reaction(PCR) method and detect porcine circovirus type 2 (PCV2) from intestines and feces of commercial swine herds with or without enteric disease, intestinal samples from 68 pigs and 29 fecal samples from commercial swine farms were collected. A primer set, forward primer 5′-GAAGAATGGAAGAAGCGG-3′ and reverse primer 5′-CTCACAGCAGTAGACAGGT-3′, could detect the virus at a concentration as low as 2 infectious virions per milliliter under controlled conditions using PK-15 cell-adapted PCV2. The genomic nucleotide sequences of open reading frame 1 (ORF1) PCR products from fecal samples were found to have complete homology with other PCV2s deposited in the GenBank database. Transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) as the other enteric pathogens were also investigated by performing duplex reverse transcription-PCR (RT-PCR). Among 63 pigs with clinical enteric disease, 18 PCV2s (14 from intestines and 4 from feces), 7 TGEVs from intestines, and 18 PEDVs (14 from intestines and 4 from feces) were detected by PCR and the duplex RT-PCR. In 34 pigs (14 from intestines and 20 from feces) without clinical enteric disease, only PCV2 was detected in 19 pigs (3 from intestines and 16 from feces). Both PEDV and PCV2 were found in 6 pigs with clinical enteric disease. Among 15 PCV2 samples that were PCR-positive, 4 were culture-positive at passage level 3 in PK-15 cells. These results reveal that PCV2 is shed through the feces of pigs without clinical enteric disease, which suggests the potentiality of the fecal–oral transmission of PCV2 in feces.

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