Evaluation of the efficacy of hydrated sodium aluminosilicate in the prevention of aflatoxin-induced hepatic cancer in rainbow trout

The use of aluminum silicates for decontaminating animal feed containing aflatoxins has yielded encouraging results in chicken and turkey poults. In contrast, very few studies have tested these substances in aquaculture. In this work, we investigated the efficacy of a trout diet containing 0.5% hydrated sodium aluminosilicate (HSAS) in protecting against contamination with aflatoxin B1. Trout were reared on these diets for one year and the experimental groups were examined monthly for hepatic presumptive preneoplastic and neoplastic lesions. Regardless of the presence of HSAS, all of the fish that received aflatoxin in their diet have shown hepatic lesions indicative of a carcinogenic process, presenting also the development of cancer in some fish. The concentration of HSAS used in this study was ineffective in preventing the onset of hepatic lesions induced by aflatoxin B1 in rainbow trout.

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Differential effect of chronic aflatoxin B1 intoxication on the growth performance and incidence of hepatic lesions in triploid and diploid rainbow trout (Oncorhynchus mykiss)

Archivos de medicina veterinaria ◽

10.4067/s0301-732x2002000200011 ◽

2002 ◽

Vol 34 (2) ◽

Cited By ~ 3

Author(s):

S ARANA ◽

Y. A TABATA ◽

M SABINO ◽

M. G RIGOLINO ◽

F. J HERNANDEZ-BLAZQUEZ

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Growth Performance ◽

Aflatoxin B1 ◽

Differential Effect ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Hepatic Lesions

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Rapid and simultaneous purification of aflatoxin B1, zearalenone and deoxynivalenol using their monoclonal antibodies and magnetic nanoparticles

Toxicological Research ◽

10.1007/s43188-020-00083-w ◽

2021 ◽

Author(s):

Hyuk-Mi Lee ◽

Hwan-Goo Kang

Keyword(s):

Monoclonal Antibodies ◽

Magnetic Nanoparticles ◽

Aflatoxin B1 ◽

Animal Feed ◽

Immunoaffinity Chromatography ◽

The Novel ◽

Buffer Solution ◽

Purification Method ◽

Recovery Rates ◽

Single Spike

AbstractTo develop a new simple and simultaneous purification method for mycotoxins in feeds and grains, magnetic nanoparticles (MNPs) conjugated with monoclonal antibodies (mAbs) against mycotoxins were used to separate aflatoxin B1 (AFB1), zearalenone (ZEA) and deoxynivalenol (DON). For a single spike of each mycotoxin into the buffer solution (16% MeOH in PBS), mean recoveries were 93.1–95.0% for AFB1 (5–20 ng/mL spiked), 87.2–96.0% for ZEA (125–500 ng/mL spiked) and 75.2–96.9% for DON (250–1,000 ng/mL spiked) by HPLC and ELISA. Recoveries of AFB1 (20 ng/mL) and ZEA (500 ng/mL) simultaneously spiked into the buffer solution were 87.0 and 99.8%, respectively. Recovery rates of AFB1/DON and DON/ZEA spiked simultaneously were 86.2%/76.6% and 92.0%/86.7%, respectively, at concentrations of 20 ng/mL AFB1, 500 ng/mL ZEA, and 1,000 ng/mL DON. Recoveries using the novel mAb–MNP conjugated system in a buffer solution simultaneously spiked with AFB1, ZEA and DON were 82.5, 94.6 and 73.4%, respectively. Recoveries of DON in animal feed were 107.7–132.5% at concentrations of 250–1,000 ng/g spiked in feed. The immunoaffinity chromatography (IAC) clean-up method was compared with the purification method using novel mAb–MNP. After fortification of animal feed with AFB1 (5, 10 and 20 ng/g feed) and ZEA (125, 250 and 500 ng/g feed), AFB1 and ZEA were purified using both the methods. In the case of the novel mAb-MNP conjugated system, mean recoveries for AFB1 were 89.4, 73.1 and 88.3% at concentrations of 5, 10 and 20 ng/g feed, respectively. For ZEA, mean recoveries were 86.7, 85.9 and 79.1% at concentrations of 125, 250 and 500 ng/g, respectively. For IAC purification, recoveries were 42.9–45.1% for AFB1 and 96.8–103.2% for ZEA. In conclusion, the present purification method using monoclonal antibodies conjugated to MNPs can be used for simple and simultaneous purification of mycotoxins from feed and maize.

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Effect of dose on the inhibition of carcinogenesis/mutagenesis by aroclor 1254 in rainbow trout fed aflatoxin B1

Journal of Toxicology and Environmental Health ◽

10.1080/15287398409530529 ◽

1984 ◽

Vol 13 (4-6) ◽

pp. 649-657 ◽

Cited By ~ 19

Author(s):

Dennis W. Shelton ◽

Jerry D. Hendricks ◽

Roger A. Coulombe ◽

George S. Bailey

Keyword(s):

Rainbow Trout ◽

Aflatoxin B1 ◽

Aroclor 1254

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The effect of indole-3-carbinol, an aflatoxin B1 hepatocarcinoma inhibitor, and other indole analogs on the rainbow trout hepatic mixed function oxidase system

Toxicology Letters ◽

10.1016/0378-4274(83)90273-4 ◽

1983 ◽

Vol 19 (1-2) ◽

pp. 133-138 ◽

Cited By ~ 18

Author(s):

T.A. Eisele ◽

G.S. Bailey ◽

J.E. Nixon

Keyword(s):

Rainbow Trout ◽

Aflatoxin B1 ◽

Mixed Function Oxidase ◽

Oxidase System ◽

Mixed Function Oxidase System

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Temperature-modulated aflatoxin B1 hepatic disposition and formation and persistence of DNA adducts in rainbow trout

Toxicology and Applied Pharmacology ◽

10.1016/0041-008x(92)90122-9 ◽

1992 ◽

Vol 113 (2) ◽

pp. 253-259 ◽

Cited By ~ 7

Author(s):

Quan Zhang ◽

Katherine Suorsa-Super ◽

Lawrence R. Curtis

Keyword(s):

Rainbow Trout ◽

Dna Adducts ◽

Aflatoxin B1 ◽

Hepatic Disposition ◽

Persistence Of Dna

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Altered Gene Response to Aflatoxin B1 in the Spleens of Susceptible and Resistant Turkeys

Toxins ◽

10.3390/toxins11050242 ◽

2019 ◽

Vol 11 (5) ◽

pp. 242 ◽

Cited By ~ 1

Author(s):

Kent M. Reed ◽

Kristelle M. Mendoza ◽

Roger A. Coulombe

Keyword(s):

Gene Expression ◽

Acute Phase ◽

Aflatoxin B1 ◽

Treatment Protocol ◽

Coagulation System ◽

Original Experiment ◽

Hepatic Expression ◽

Threshold Trait ◽

Turkey Poults ◽

Wild Turkeys

Susceptibility and/or resistance to aflatoxin B1 (AFB1) is a threshold trait governed principally by glutathione S transferase (GST)-mediated detoxification. In poultry, domesticated turkeys are highly sensitive to AFB1, most likely due to dysfunction in hepatic GSTs. In contrast, wild turkeys are comparatively resistant to aflatoxicosis due to the presence of functional hepatic GSTAs and other possible physiological and immunological interactions. The underlying genetic basis for the disparate GST function in turkeys is unknown as are the broader molecular interactions that control the systemic response. This study quantifies the effects of dietary AFB1 on gene expression in the turkey spleen, specifically contrasting genetically distinct domesticated (DT, susceptible) and Eastern wild (EW, resistant) birds. Male turkey poults were subjected to a short-term AFB1 treatment protocol with feed supplemented with 320 ppb AFB1 beginning on day 15 of age and continuing for 14 days. Spleen tissues were harvested and subjected to deep RNA sequencing and transcriptome analysis. Analysis of differential gene expression found the effects of AFB1 treatment on the spleen transcriptomes considerably more prominent in the DT birds compared to EW. However, expression of the differentially expressed genes (DEGs) was directionally biased, with the majority showing higher expression in EW (i.e., down-regulation in DT). Significantly altered pathways included FXR/RXR and LXR/RXR activation, coagulation system, prothrombin activation, acute phase response, and atherosclerosis signaling. Differential extra-hepatic expression of acute phase protein genes was confirmed by quantitative real time PCR (qRT-PCR) in the original experiment and additional turkey lines. Results demonstrate that wild turkeys possess a capacity to more effectively respond to AFB1 exposure.

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