scholarly journals 5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)

2010 ◽  
Vol 43 (12) ◽  
pp. 1143-1152 ◽  
Author(s):  
J.C. de Almeida ◽  
H. Sauaia ◽  
J.C. Viana
Keyword(s):  
Salivary Gland ◽  
Morphological Alteration ◽  
Dna Puffs ◽  
Bradysia Hygida

The giant DNA puffs of Rhynchosciara americana code for polypeptides of the salivary gland secretion

1977 ◽  
Vol 23 (11-12) ◽  
pp. 1455-1459 ◽  
Author(s):  
C.E. Winter ◽  
A.G. de Bianchi ◽  
W.R. Terra ◽  
F.J.S. Lara
Keyword(s):  
Salivary Gland ◽  
Gland Secretion ◽  
Salivary Gland Secretion ◽  
Rhynchosciara Americana ◽  
Dna Puffs

Indirect immune detection of ecdysone receptor (EcR) during the formation of DNA puffs in Bradysia hygida (Diptera, Sciaridae)

2008 ◽  
Vol 16 (4) ◽  
pp. 609-622 ◽  
Author(s):  
Juliana Aparecida Candido-Silva ◽  
Daniela Palermo de Carvalho ◽  
Graciana Rodrigues Coelho ◽  
Jorge Cury de Almeida
Keyword(s):  
Ecdysone Receptor ◽  
Dna Puffs ◽  
Immune Detection ◽  
Bradysia Hygida

scholarly journals Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells

2005 ◽  
Vol 48 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Celso Aparecido Polinarski ◽  
José Luis da Conceição Silva ◽  
Liya Regina Mikami ◽  
Maria Aparecida Fernandez
Keyword(s):  
Salivary Gland ◽  
Structure And Function ◽  
Confocal Laser ◽  
Work Analysis ◽  
Confocal Laser Scan Microscopy ◽  
Nuclear Halo ◽  
Hypotonic Buffer ◽  
And Function ◽  
Polytene Nuclei ◽  
Bradysia Hygida

A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles.

Development of DNA puffs and patterns of polypeptide synthesis in the salivary glands of Bradysia hygida

Chromosoma ◽  
1984 ◽  
Vol 89 (4) ◽  
pp. 280-284 ◽  
Author(s):  
Eduardo M. Laicine ◽  
Maurílio A. R. Alves ◽  
Jorge C. de Almeida ◽  
Elizete Rizzo ◽  
Wander C. Albernaz ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Polypeptide Synthesis ◽  
Dna Puffs ◽  
Bradysia Hygida

The control of BhB10-1 gene expression in the salivary gland of Bradysia hygida (Diptera, Sciaridae) is disrupted in vivo by a delayed effect of cycloheximide

2002 ◽  
Vol 32 (7) ◽  
pp. 737-745 ◽  
Author(s):  
M.E. Conacci ◽  
P.S.R. Coelho ◽  
V. Valente ◽  
J. de F. Sousa ◽  
M.L. Paçó-Larson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salivary Gland ◽  
Delayed Effect ◽  
Bradysia Hygida

The allometric relationship between nucleus and cytoplasm in the salivary glands of Bradysia hygida

1983 ◽  
Vol 61 (6) ◽  
pp. 1311-1316
Author(s):  
Heid Sueli Leme dos Santos ◽  
André Ricciardi Cruz
Keyword(s):  
Larval Stage ◽  
Polytene Chromosomes ◽  
Critical Time ◽  
Functional Changes ◽  
Allometric Relations ◽  
Fourth Larval Stage ◽  
Glandular Cells ◽  
Allometric Relation ◽  
Dna Puffs ◽  
Bradysia Hygida

The volumetric relationship between nucleus and cytoplasm of the Bradysia hygida salivary gland cells was studied during development using an allometric relation. This relationship during the fourth larval stage is defined by a simple allometric relation. Cell growth, differentiation, and maturation during the fourth larval stage takes place in two distinct steps separated by an interphase occurring between the 6th and 8th day after the third moult. This interphase marks a critical time of development and coincides with the occurrence of DNA puffs on the polytene chromosomes of the glandular cells. Although the allometric coefficient does not change over two distinct developmental steps (k = 1), our observations support the hypothesis that the interphase observed in the allometric relations is subject to the appearance of DNA puffs and is related to functional changes in the gland.

Segments of chromosomal DNA from Rhynchosciara americana that undergo additional rounds of DNA replication in the salivary gland DNA puffs have only weak ARS activity in yeast

Gene ◽  
1985 ◽  
Vol 34 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Sarah Millar ◽  
David C. Hayward ◽  
Christopher A. Read ◽  
Michael J. Browne ◽  
Roberto V. Santelli ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Dna Replication ◽  
Chromosomal Dna ◽  
Rhynchosciara Americana ◽  
Dna Puffs

Amplification and expression of a salivary gland DNA puff gene in the prothoracic gland of Bradysia hygida (Diptera: Sciaridae)

2015 ◽  
Vol 74 ◽  
pp. 30-37 ◽  
Author(s):  
Juliana Aparecida Candido-Silva ◽  
Maiaro Cabral Rosa Machado ◽  
Klaus Hartmann Hartfelder ◽  
Jorge Cury de Almeida ◽  
Maria Luisa Paçó-Larson ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Prothoracic Gland ◽  
Bradysia Hygida
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