scholarly journals Characterization of pepper accessions using molecular markers linked to pungency and SSR

2019 ◽  
Vol 37 (2) ◽  
pp. 152-160
Author(s):  
Rafaela de Jesus ◽  
Gabriel do N Santos ◽  
Andressa S Piccin ◽  
Thiago WA Balsalobre ◽  
Fernando C Sala ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Markers ◽  
Microsatellite Markers ◽  
Structure Analysis ◽  
Breeding Programs ◽  
Population Structure Analysis ◽  
Level Information ◽  
Capsicum Spp ◽  
Genus Capsicum

ABSTRACT Peppers of the genus Capsicum are of great socioeconomic importance, being pungency trait their main attraction. Pungency characterization, genetic distance estimates and population structure analysis of the accessions belonging to germplasm banks are important for parent selection which allows to obtain superior progenies. Therefore, the aims of this study were: i) evaluate 81 accessions of the Capsicum spp. Germplasm Bank of Universidade Federal de São Carlos (BGC-UFSCar) with molecular markers linked to pungency; ii) estimate the genetic diversity among accessions of the BGC-UFSCar using microsatellite markers (SSR); and iii) evaluate the efficiency of these markers in the distinction among species of Capsicum spp. We noticed that pun11 and SNP molecular markers were efficient in predicting the pungent phenotype of BGC-UFSCar accessions in 84.85% and 95.59%, respectively. From a total of 13 amplified microsatellite markers, seven were polymorphic and efficient to discriminate species of Capsicum genus, both through genetic diversity analysis and population structure analysis, which showed three subpopulations. The molecular markers used in this study are useful tools for breeding programs since they were able to characterize and discriminate Capsicum spp. species at DNA level. Information obtained with molecular markers can assist in the selection of contrasting parents for future breeding programs.

scholarly journals Genetic diversity of the Colombian Creole donkey in the Department of Sucre

2020 ◽  
Vol 51 (6) ◽  
pp. 611-619
Author(s):  
Adrian Medina-Montes ◽  
Darwin Hernández-Herrera ◽  
Javier Beltrán-Herrera ◽  
Donicer Montes-Vergara
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Molecular Markers ◽  
Structure Analysis ◽  
Genetic Distances ◽  
Entire Population ◽  
Polymorphic Loci ◽  
Population Structure Analysis

The objective of this study was to evaluate the genetic diversity of the Colombian Creole donkey in the Department of Sucre using Random Amplified Microsatellites (RAM) molecular markers. In 100 individuals from the five subregions of the department, DNA was extracted and five RAM primers were amplified by PCR. In all, 291 bands were found, on average 11.96±1.45 per primer, the highest value in CCA (18±2.23) and the lowest in TG and GT (8.8±0.44). CA was the most polymorphic primer (88.09±10.91%) with the highest heterozygosity value (He) (0.376±0.021), while the lowest was GT (0.341±0.076 and 0.101±0.040, respectively). Intrapopulation analysis showed an average of 66.50±1.72 bands, of which 89.86±24.04% were polymorphic. The highest number of bands (63±3.84) was found in the Gulf of Morrosquillo (GO) subpopulation, and the lowest in Mojana (MO) (48±2.88); however, the highest value of polymorphic loci (81.16%) and He (0.335±0.022) were found in the Montes de María (MM) subpopulation, making it the most diverse. The average genetic diversity for the entire population was 0.351±0.021 bands. The population structure analysis showed a 10% variation between subpopulations, with an FST value of 0.17±0.01 (P<0.05). Genetic distances between subpopulations showed that MO and GO were the most distant. The RAM markers are effective in assessing the genetic diversity of the Creole donkey, which has high values of genetic diversity, particularly the MM subpopulation. The genetic revealed structure could be the result of natural geographical barriers between the subregions.

scholarly journals Genetic Diversity and Population Structure Analysis in Early Generations Maize Inbreds Derived From Local Germplasm of Eastern Himalayan Regions Using Microsatellite Markers

2022 ◽  
Author(s):  
E LAMALAKSHMI DEVI ◽  
Umakanta Ngangkham ◽  
Akoijam Ratankumar Singh ◽  
Bhuvaneswari S ◽  
Konsam Sarika ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Structure Analysis ◽  
Agronomic Traits ◽  
Crop Improvement ◽  
Marker Locus ◽  
Genetic Enhancement ◽  
Population Structure Analysis ◽  
Maize Inbreds

Abstract North- Eastern parts of India fall under the Eastern Himalayan region and it is a diversity hotspot of many crops, including maize. Maize is an important traditional cereal crop grown in hill ecology of the region mainly for food, fodder and feed. To tap the potentiality of maize genetic resources in crop improvement programmes, assessment of genetic diversity is a basic requirement. Hence, in the present study, assessment of genetic diversity in thirty early generation maize inbreds developed from different germplasm of NE India was taken up using genome wide distributed fifty two microsatellite markers. The marker analysis revealed a large variation with a total of 189 alleles with an average of 3.63 alleles per marker locus. The allele size ranged from 50 bp ( phi 036 ) to 295 bp ( p 101049 ) which revealed a high level of genetic diversity among the loci. The PIC value ranged from 0.17 ( umc 1622 ) to 0.76 ( umc 1153 ) with an average value of 0.49. The value of expected Heterozygosity (H Exp ) ranged from 0.19 to 0.80 with an average of 0.57, whereas the Observed Heterozygosity (H Obs ) ranged from 0 to 0.89 with a mean of 0.14.The genetic dissimilarity between the genotype pairs ranged from 0.40 to 0.64 with a mean value of 0.57. Cluster analysis resolved the inbreds into three distinct sub-clusters. Similarly, population structure analysis also classified the inbred lines into three-subpopulations. Marker-trait associations showed a total of twelve SSR markers significantly associated with seven agronomic traits. From the present study, wide genetic variability was found among the maize inbreds with high potential to contribute new beneficial and unique alleles in genetic enhancement program of maize in India and particularly, in NE region.

Genetic diversity and population structure analysis of mango (Mangifera indica) cultivars assessed by microsatellite markers

Trees ◽  
2015 ◽  
Vol 29 (3) ◽  
pp. 775-783 ◽  
Author(s):  
Kundapura V. Ravishankar ◽  
Padmakar Bommisetty ◽  
Anju Bajpai ◽  
Navin Srivastava ◽  
Bellam H. Mani ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Structure Analysis ◽  
Mangifera Indica ◽  
Population Structure Analysis

scholarly journals Genetic diversity and population structure analysis of Lateolabrax maculatus from Chinese coastal waters using polymorphic microsatellite markers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei Wang ◽  
Chunyan Ma ◽  
Longling Ouyang ◽  
Wei Chen ◽  
Ming Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Structure Analysis ◽  
Principal Component ◽  
Genetic Constitution ◽  
Southern Group ◽  
Population Structure Analysis ◽  
Lateolabrax Maculatus ◽  
Geographic Populations ◽  
Geographic Separation

AbstractIn order to provide valuable guidelines for the conservation of germplasm of Lateolabrax maculatus, the genetic diversity and population structure analysis were evaluated for eight geographic populations along coastal regions of China, using 11 microsatellite DNA markers. The genetic parameters obtained showed that, eight populations can be clustered into two groups, the Northern group and the Southern group, concordant with their geographical positions. The UPGMA tree constructed according to the Nei’s genetic distance along with the structure analysis and discriminant analysis of principal component also supported this result. This might be explained by the geographic separation and the divergent environmental conditions among the populations. It's worth noting that, QD (Qingdao) population from northern area was assigned to the Southern group and showed a close genetic relationship and similar genetic constitution with the southern populations. We speculated that large scales of anthropogenic transportation of wild fries from QD populations to the southern aquaculture areas in history should be the primary cause. The populations from GY (Ganyu), RD (Rudong) and BH (Binhai) had higher genetic diversity and showed limited genetic exchange with other populations, indicating better conservation of the natural resources in these regions. All populations were indicated to have experienced bottleneck events in history.

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