scholarly journals Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease

2006 ◽  
Vol 124 (3) ◽  
pp. 154-157 ◽  
Author(s):  
Juliane Garcez Musacchio ◽  
Maria da Glória da Costa Carvalho ◽  
José Carlos Oliveira de Morais ◽  
Nathalie Henriques Silva ◽  
Adriana Scheliga ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Rio De Janeiro ◽  
Epstein Barr Virus ◽  
Advanced Disease ◽  
Hodgkin’S Disease ◽  
Hiv Positive ◽  
Control Group ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

CONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkin’s disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkin’s disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkin’s disease, and in all patients with HIV-associated Hodgkin’s disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status.

HIGH FREQUENCY OF EPSTEIN-BARR VIRUS GENOME IN HIV-POSITIVE PATIENTS WITH HODGKIN'S DISEASE

The Lancet ◽  
1989 ◽  
Vol 333 (8652) ◽  
pp. 1458 ◽  
Author(s):  
Stefania Uccini ◽  
Francesca Monardo ◽  
LuigiP. Ruco ◽  
CarloD. Baroni ◽  
Alberto Faggioni ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
High Frequency ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Virus Genome ◽  
Hiv Positive ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1595-1602 ◽  
Author(s):  
ML Gulley ◽  
PA Eagan ◽  
L Quintanilla-Martinez ◽  
AL Picado ◽  
BN Smir ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Mixed Cellularity ◽  
Hispanic Ethnicity ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed- Sternberg (RS) cells of mixed cellularity Hodgkin's disease (37 of 48 [77%]) compared with the nodular sclerosis subtype (19 of 71 [27%], P < .001). The presence of EBV was also associated with Hispanic ethnicity (P < .001). In a multivariate analysis, patient age, gender, and geographic location were less predictive of EBV positivity than were mixed cellularity histology (odds ratio = 8.3) and Hispanic ethnicity (odds ratio = 4.3). Southern blot analysis of EBV terminal repeat fragments using the Xho1a probe showed that the viral DNA was monoclonal in 17 of 17 cases having EBER1-positive RS cells. By comparison, EBV DNA was not detected by Southern analysis in 20 cases lacking EBER1 in RS cells, even when occasional background lymphocytes expressed EBER1. Because clonal viral DNA was so readily detected in EBER1-positive cases, the EBV genome is probably amplified at least 50- fold in the infected RS cells. Monoclonality of EBV DNA implies that the RS cells were infected before malignant transformation.

scholarly journals Epstein-Barr virus DNA is abundant and monoclonal in the Reed-Sternberg cells of Hodgkin's disease: association with mixed cellularity subtype and Hispanic American ethnicity

Blood ◽  
1994 ◽  
Vol 83 (6) ◽  
pp. 1595-1602 ◽  
Author(s):  
ML Gulley ◽  
PA Eagan ◽  
L Quintanilla-Martinez ◽  
AL Picado ◽  
BN Smir ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Viral Dna ◽  
Mixed Cellularity ◽  
Hispanic Ethnicity ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Abstract One hundred twenty-five cases of Hodgkin's disease from the United States (79), Mexico City (31), and Costa Rica (15) were analyzed for the presence of Epstein-Barr virus (EBV) by in situ hybridization to EBER1 transcripts. EBV was more frequently detected in the Reed- Sternberg (RS) cells of mixed cellularity Hodgkin's disease (37 of 48 [77%]) compared with the nodular sclerosis subtype (19 of 71 [27%], P < .001). The presence of EBV was also associated with Hispanic ethnicity (P < .001). In a multivariate analysis, patient age, gender, and geographic location were less predictive of EBV positivity than were mixed cellularity histology (odds ratio = 8.3) and Hispanic ethnicity (odds ratio = 4.3). Southern blot analysis of EBV terminal repeat fragments using the Xho1a probe showed that the viral DNA was monoclonal in 17 of 17 cases having EBER1-positive RS cells. By comparison, EBV DNA was not detected by Southern analysis in 20 cases lacking EBER1 in RS cells, even when occasional background lymphocytes expressed EBER1. Because clonal viral DNA was so readily detected in EBER1-positive cases, the EBV genome is probably amplified at least 50- fold in the infected RS cells. Monoclonality of EBV DNA implies that the RS cells were infected before malignant transformation.

scholarly journals Expression of Epstein-Barr virus in patients with Hodgkin's disease: report of 64 cases from Rio de Janeiro, Brazil

2004 ◽  
Vol 40 (1) ◽  
Author(s):  
Monique Morgado Loureiro ◽  
José Carlos Morais ◽  
Cristiane Bedran Milito ◽  
Rodrigo Doyle Portugal ◽  
Wolmar Pulcheri ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Rio De Janeiro ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Barr Virus ◽  
Epstein Barr

High frequency of Epstein-Barr virus genome detection in Hodgkin's disease of HIV-positive patients

1990 ◽  
Vol 46 (4) ◽  
pp. 581-585 ◽  
Author(s):  
Stefania Uccini ◽  
Francesca Monardo ◽  
Antonella Stoppacciaro ◽  
Angela Gradilone ◽  
Anna M. Aglianò ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
High Frequency ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Virus Genome ◽  
Hiv Positive ◽  
Barr Virus ◽  
Epstein Barr

Influence of Epstein–Barr Virus Genomes on Patient Survival in Hodgkin’s Disease

1992 ◽  
Vol 98 (3) ◽  
pp. 319-323 ◽  
Author(s):  
Christian Fellbaum ◽  
Martin-Leo Hansmann ◽  
Hans Niedermeyer ◽  
Irmgard Kraus ◽  
Martti J. Alavaikko ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Epstein Barr Virus ◽  
Patient Survival ◽  
Hodgkin’S Disease ◽  
Barr Virus ◽  
Epstein Barr ◽  
Virus Genomes

Comparison of Epstein-Barr Virus Markers in Reed-Sternberg Cells in Adult Hodgkin's Disease Tissues from an Industrialized and a Developing Country

1995 ◽  
Vol 17 (1-2) ◽  
pp. 163-168 ◽  
Author(s):  
Mohammed I. Belkaid ◽  
Josette Briegre ◽  
Zahia Djebbara ◽  
Kheira Beldjord ◽  
Jean Marie Andrieu ◽  
...  
Keyword(s):  
Developing Country ◽  
Hodgkin's Disease ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein–Barr virus gene polymorphisms in Chinese Hodgkin’s disease cases and healthy donors: identification of three distinct virus variants

2001 ◽  
Vol 82 (5) ◽  
pp. 1157-1167 ◽  
Author(s):  
X.-G. Zhou ◽  
K. Sandvej ◽  
P.-J. Li ◽  
X.-L. Ji ◽  
Q.-H. Yan ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Epstein Barr Virus ◽  
Hodgkin’S Disease ◽  
Epidemiological Studies ◽  
Latent Membrane Protein 1 ◽  
Barr Virus ◽  
Healthy Donors ◽  
Healthy Chinese ◽  
Epstein Barr ◽  
Virus Isolates

Epstein–Barr virus (EBV) is associated with several malignancies. Specific EBV gene variants, e.g. the BamHI f configuration, a C-terminal region 30 bp deletion in the latent membrane protein-1 (LMP1) gene (del-LMP) and the loss of an XhoI site in LMP1 (XhoI-loss), are found in Chinese cases of nasopharyngeal carcinoma (NPC), suggesting that EBV sequence variation may be involved in oncogenesis. In order to understand better the epidemiology of these EBV variants, they were studied in virus isolates from EBV-positive Chinese cases of Hodgkin’s disease (HD; n=71) and donor throat washings from healthy Chinese. Sequencing was performed of 15 representative EBV isolates, including the first analysis of the LMP1 promoter in Asian wild-type EBV isolates. The following observations were made. (i) Three EBV LMP1 variants were identified, designated Chinese groups (CG) 1–3. In both EBV-associated HD and in healthy Chinese, CG1-like viruses showing del-LMP1 and XhoI-loss were predominant. (ii) CG1viruses were distinct from European and African variants, suggesting that this profile is useful for epidemiological studies. (iii) Specific patterns of mutations were present in the LMP1 promoter in both CG1 and CG2. (iv) The BamHI f variant was not found in Chinese HD, in contrast to Chinese NPC and European HD. This study confirms that EBV isolates in Chinese HD and other tumours differ from those reported in Western cases. However, this reflects the predominant virus strain present in the healthy Chinese population, suggesting that these are geographically restricted polymorphisms rather than tumour-specific strains.

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