Identification and colonization of endophytic fungi from soybean (Glycine max (L.) Merril) under different environmental conditions

A total of 297 endophytic fungi were isolated from 1728 leaf and stem fragments collected about twenty and forty days after germination from soybean (Glycine max (L.) Merril) plants grown in the field and a greenhouse. The fungi belonged to eight groups, six dematiaceous genera (Alternaria, Cladosporium, Chaetomium, Curvularia, Drechslera and Scopulariopsis) and the non-dematiaceous genera Acremonium, Aspergillus, Colletotrichum, Fusarium, Paecilomyces and Penicillium along with some Mycelia sterilia.. There were qualitative and quantitative differences in the type and number of isolates obtained from greenhouse and field-grown plants, with more isolates being obtained from the latter. No difference was found in the number of fungi isolated from leaves and stems irrespective of where the plants was grown. For was field-grown plants, the number of isolates decreased as the plants aged and more fungi were found in tissues near the soil, while for greenhouse-grown plants the number of isolates increased as the plants aged but in this case no more fungi were isolated from those tissues nearer the soil. These results could have biotechnological relevance for the biological control of pests or plant growth promotion.

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Plant growth-promotion (PGP) activities and molecular characterization of rhizobacterial strains isolated from soybean (Glycine max L. Merril) plants against charcoal rot pathogen, Macrophomina phaseolina

Biotechnology Letters ◽

10.1007/s10529-011-0699-0 ◽

2011 ◽

Vol 33 (11) ◽

pp. 2287-2295 ◽

Cited By ~ 24

Author(s):

D. K. Choudhary

Keyword(s):

Glycine Max ◽

Plant Growth ◽

Molecular Characterization ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Macrophomina Phaseolina ◽

Charcoal Rot ◽

Glycine Max L ◽

Pgp Activities

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Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice

Canadian Journal of Microbiology ◽

10.1139/w01-097 ◽

2001 ◽

Vol 47 (10) ◽

pp. 916-924 ◽

Cited By ~ 71

Author(s):

Tika B Adhikari ◽

C M Joseph ◽

Guoping Yang ◽

Donald A Phillips ◽

Louise M Nelson

Keyword(s):

Biological Control ◽

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Disease Incidence ◽

Control Plant ◽

16S Rrna Genes ◽

Rrna Genes ◽

Bacterial Strains ◽

Seedling Disease

Of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in California, 37% significantly (P [Formula: see text] 0.05) inhibited the growth in vitro of two pathogens, Achlya klebsiana and Pythium spinosum, causing seedling disease of rice. Four endophytic strains were highly effective against seedling disease in growth pouch assays, and these were identified as Pseudomonas fluorescens (S3), Pseudomonas tolaasii (S20), Pseudomonas veronii (S21), and Sphingomonas trueperi (S12) by sequencing of amplified 16S rRNA genes. Strains S12, S20, and S21 contained the nitrogen fixation gene, nifD, but only S12 was able to reduce acetylene in pure culture. The four strains significantly enhanced plant growth in the absence of pathogens, as evidenced by increases in plant height and dry weight of inoculated rice seedlings relative to noninoculated rice. Three bacterial strains (S3, S20, and S21) were evaluated in pot bioassays and reduced disease incidence by 50%73%. Strain S3 was as effective at suppressing disease at the lowest inoculum density (106 CFU/mL) as at higher density (108 CFU/mL or undiluted suspension). This study indicates that selected endophytic bacterial strains have potential for control of seedling disease of rice and for plant growth promotion.Key words: biological control, plant growth promotion, endophytes, rice, seedling disease.

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