scholarly journals Bacteriocin like substance production by Carnobacterium piscicola in a continuous system with three culture broths. Study of antagonism against Listeria monocytogenes on vacuum packaged salmon

2006 ◽  
Vol 37 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Renate P. Schöbitz ◽  
Pamela A. Bórquez ◽  
Marcia E. Costa ◽  
Luigi R. Ciampi ◽  
Carmen S. Brito
Keyword(s):  
Listeria Monocytogenes ◽  
Continuous System ◽  
Carnobacterium Piscicola

Antilisterial Activity of a Carnobacterium piscicola Isolated from Brazilian Smoked Fish (Surubim [Pseudoplatystoma sp.]) and Its Activity against a Persistent Strain of Listeria monocytogenes Isolated from Surubim

2005 ◽  
Vol 68 (10) ◽  
pp. 2068-2077 ◽  
Author(s):  
VIRGÍNIA F. ALVES ◽  
ELAINE C. P. DE MARTINIS ◽  
MARIA TERESA DESTRO ◽  
BIRTE FONNESBECH VOGEL ◽  
LONE GRAM
Keyword(s):  
Listeria Monocytogenes ◽  
Freshwater Fish ◽  
Random Amplified Polymorphic Dna ◽  
Model Systems ◽  
Fish Products ◽  
Tropical Regions ◽  
Smoked Fish ◽  
Smoked Salmon ◽  
Carnobacterium Piscicola

Data on the prevalence and growth of Listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. Our research describes L. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native Brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic DNA subtype as the strains that were isolated from the same factory 4 years earlier. A bacteriocinogenic strain of Carnobacterium piscicola (strain C2), isolated from vacuum-packed cold-smoked surubim, and two C. piscicola strains, isolated from vacuum-packed, cold-smoked salmon, were capable of limiting or completely inhibiting the growth of an L. monocytogenes (strain V2) isolated from surubim in fish peptone model systems incubated at 10°C. Mono-cultures of L. monocytogenes reached 108 CFU/ml (g), whereas the growth of L. monocytogenes was completely inhibited by C. piscicola C2. The bacteriocinogenic C. piscicola A9b+ and its nonbacteriocinogenic mutant A9b− reduced maximum Listeria levels by 2 to 3 log units. Both bacteriocinogenic C. piscicola strains prevented listerial growth in cold-smoked fish juices (surubim and salmon). Although the carnobacteria grew poorly on cold-smoked surubim at 10°C, the strains were able to reduce maximum Listeria counts by 1 to 3 log units in an artificially inoculated product (surubim). We conclude that Brazilian smoked fish products harbor L. monocytogenes and should be stabilized against the growth of the organism. C. piscicola C2 has the potential for use as a bioprotective culture in surubim and other lightly preserved fish, but further studies are required to optimize its effect.

scholarly journals The contribution of bacteriocin to inhibition of Listeria monocytogenes by Carnobacterium piscicola strains in cold-smoked salmon systems

2004 ◽  
Vol 96 (1) ◽  
pp. 133-143 ◽  
Author(s):  
L. Nilsson ◽  
Y.Y. Ng ◽  
J.N. Christiansen ◽  
B.L. Jorgensen ◽  
D. Grotinum ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Smoked Salmon ◽  
Carnobacterium Piscicola

Inhibition of Listeria monocytogenes by In Situ Produced and Semipurified Bacteriocins of Carnobacterium spp. on Vacuum-Packed, Refrigerated Cold-Smoked Salmon

1999 ◽  
Vol 62 (12) ◽  
pp. 1394-1403 ◽  
Author(s):  
FRÉDÉRIQUE DUFFES ◽  
CHRISTIAN CORRE ◽  
FRANÇOISE LEROI ◽  
XAVIER DOUSSET ◽  
PATRICK BOYAVAL
Keyword(s):  
Listeria Monocytogenes ◽  
Biogenic Amine ◽  
Bacteriostatic Effect ◽  
Crude Extracts ◽  
Smoked Salmon ◽  
Carnobacterium Piscicola ◽  
Two Temperatures ◽  
Sensorial Analyses ◽  
Carnobacterium Divergens

Listeria monocytogenes inhibition by Carnobacterium strains and crude bacteriocins on sterile and commercial vacuum-packed cold-smoked salmon stored at 4°C and 8°C was investigated. Carnobacterium piscicola V1 was bactericidal against L. monocytogenes at the two temperatures, whereas Carnobacterium divergens V41 presented a bacteriostatic effect. C. piscicola SF668 delayed L. monocytogenes growth at 8°C and had a bacteriostatic effect at 4°C. Listeria growth was not affected by a non–bacteriocin-producing C. piscicola. Crude extracts of piscicocins were bactericidal at 4°C and 8°C. Listeria growth was delayed by divercin V41 at 8°C and was inhibited at 4°C. Nisin delayed Listeria growth at 8°C and was bacteriostatic at 4°C. The present study demonstrates that L. monocytogenes growth could be prevented on vacuum-packed cold-smoked salmon by Carnobacterium and associated bacteriocins at chilled temperatures. Moreover, no product spoilage could be observed with the use of such bacteriocin-producing strains as demonstrated by good sensorial analyses and low biogenic amine production.

Inhibition of Listeria monocytogenes in Cold-Smoked Salmon by Carnobacterium piscicola CS526 Isolated from Frozen Surimi

2003 ◽  
Vol 66 (8) ◽  
pp. 1420-1425 ◽  
Author(s):  
KOJI YAMAZAKI ◽  
MINAKO SUZUKI ◽  
YUJI KAWAI ◽  
NORIO INOUE ◽  
THOMAS J. MONTVILLE
Keyword(s):  
Listeria Monocytogenes ◽  
Sequence Similarity ◽  
Cell Number ◽  
Culture Broth ◽  
Inoculum Level ◽  
Initial Inoculum ◽  
Smoked Salmon ◽  
Colony Forming Units ◽  
Carnobacterium Piscicola ◽  
Frozen Surimi

Strain CS526 was isolated from frozen surimi and identified as a bacteriocin producer that had strong inhibitory activity against Listeria monocytogenes. Strain CS526 was identified as Carnobacterium piscicola by partial 16S rDNA sequence similarity. The ability of this bacteriocinogenic strain and nonbacteriocinogenic C. piscicola JCM5348 to inhibit the growth of L. monocytogenes was examined in culture broth incubated at 12°C and cold-smoked salmon stored at 4, 12, and 20°C. L. monocytogenes viable counts in the culture broth rapidly declined from 106 colony-forming units per ml to less than 10 colony-forming units per ml within 1 day at 12°C in the presence of C. piscicola CS526. At 4 and 12°C, inhibition of L. monocytogenes on salmon depended on the initial inoculum level of C. piscicola CS526. However, C. piscicola CS526 was bactericidal to L. monocytogenes within 21 and 12 days at 4 and 12°C in cold-smoked salmon, respectively, even when the initial inoculum levels were low. C. piscicola CS526 suppressed the maximum cell number of L. monocytogenes by two and three log cycles, even at 20°C. However, C. piscicola JCM5348 did not prevent the growth of the pathogen, except at 4°C. Bacteriocin was detected in the samples coinoculated with C. piscicola CS526. The study shows that C. piscicola CS526 might have potential for biopreservation of refrigerated foods against L. monocytogenes.

Microbial Competition: Effect of Culture Conditions on the Suppression of Listeria monocytogenes Scott A by Carnobacterium piscicola†

1997 ◽  
Vol 60 (3) ◽  
pp. 254-261 ◽  
Author(s):  
ROBERT L. BUCHANAN ◽  
LORI K. BAGI
Keyword(s):  
Listeria Monocytogenes ◽  
Brain Heart Infusion ◽  
Oxygen Depletion ◽  
Culture Conditions ◽  
Brain Heart Infusion Broth ◽  
Microbial Competition ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Carnobacterium Piscicola ◽  
Effects Of Temperature

The effects of temperature (4,12, and 19°C), pH (5, 6, and 7), and NaCl (5, 25, and 45 g/liter) on the growth of Listeria monocytogenes Scott A in the presence of either Camobacterium piscicola LK5 or 2762 were studied quantitatively in brain heart infusion broth. Strain LK5 produces a bacteriocin that is released into the environment, whereas 2762 appears to produce a bacteriocin that remains cell associated. The primary effect of both C. piscicola strains was a suppression of the maximum population density (MPD) attained by L. monocytogenes. The extent of this depression was dependent on the three culture variables, and appeared to be a function of their influence on the relative growth rates of the two species. The effects were similar with both strains. However, two bacteriocin-negative strains, 2305 and 2818, also depressed the growth of L. monocytogenes. Little of the C. piscicola isolates' ability to suppress L. monocytogenes appeared attributable to bacteriocin production. The MPD-depressing activity of 2762 could not be attributed to peroxide, pH depression, or oxygen depletion. However, MPD suppression may involve nutrient depletion, since the extent of MPD suppression was decreased in a dose-related manner when the two species were cultured in 3 × and 6× brain heart infusion broth.

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