scholarly journals Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules

2010 ◽  
Vol 46 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Rudy Bonfilio ◽  
Lívia Botacini Favoretto ◽  
Gislaine Ribeiro Pereira ◽  
Roberta de Cássia Pimentel Azevedo ◽  
Magali Benjamim de Araújo
Keyword(s):  
Analytical Methods ◽  
Low Cost ◽  
Zero Order ◽  
Losartan Potassium ◽  
Angiotensin Ii Receptors ◽  
Uv Spectrophotometry ◽  
First Derivative ◽  
Order Spectrum ◽  
First Derivative Spectrophotometry ◽  
Derivative Uv Spectrophotometry

Losartan potassium is an antihypertensive non-peptide agent, which exerts its action by specific blockade of angiotensin II receptors. The aim of the present study was the validation and application of analytical methods for the quality control of losartan potassium 50 mg in pharmaceutical capsules, using direct and first-derivative UV spectrophotometry. Based on losartan potassium spectrophotometric characteristics, a signal at 205 nm of the zero-order spectrum and a signal at 234 nm of the first-derivative spectrum, were found adequate for quantification. The results were used to compare these instrumental techniques. The linearity between the signals and concentrations of losartan potassium in the ranges of 3.0-7.0 mg L-1 and 6.0-14.0 mg L-1 for direct and first-derivative spectrophotometry in aqueous solutions, respectively, presented a correlation coefficient (r) of 0.9999 in both cases. The methods were applied for losartan potassium in capsule dosage obtained from local pharmacies, and were shown to be efficient, easy to apply and low cost. These methods do not use polluting reagents and require relatively inexpensive equipment.

scholarly journals Simultaneous Determination of Amlodipine and Valsartan

2011 ◽  
Vol 6 ◽  
pp. ACI.S7282 ◽  
Author(s):  
Nashwah Gadallah Mohamed
Keyword(s):  
Absorption Spectrum ◽  
Simultaneous Determination ◽  
Zero Order ◽  
Uv Spectrophotometry ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Bulk Powder ◽  
Overlapped Peaks ◽  
Third Derivative

A spectrophotometric method was developed for simultaneous determination of amlodipine (Aml) and valsartan (Val) without previous separation. In this method amlodipine in methanolic solution was determined using zero order UV spectrophotometry by measuring its absorbency at 360.5 nm without any interference from valsartan. Valsartan spectrum in zero order is totally overlapped with that of amlodipine. First, second and third derivative could not resolve the overlapped peaks. The first derivative of the ratio spectra technique was applied for the measurement of valsartan. The ratio spectrum was obtained by dividing the absorption spectrum of the mixture by that of amlodipine, so that the concentration of valsartan could be determined from the first derivative of the ratio spectrum at 290 nm. Quantification limits of amlodipine and valsartan were 10-80 μg/ml and 20-180 μg/ml respectively. The method was successfully applied for the quantitative determination of both drugs in bulk powder and pharmaceutical formulation.

scholarly journals Development and Validation of Dissolution Test for Fluconazole Capsules by HPLC and Derivative UV Spectrophotometry

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Josilene Chaves Ruela Corrêa ◽  
Cristina Duarte Vianna-Soares ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Release Profile ◽  
New Drugs ◽  
Uv Spectrophotometry ◽  
Dissolution Test ◽  
Registration Process ◽  
Test Parameters ◽  
First Derivative Spectrophotometry ◽  
Analysis Application ◽  
Development And Validation ◽  
Derivative Uv Spectrophotometry

The purpose of this study is to develop and validate a dissolution test for fluconazole, an antifungal used for the treatment of superficial, cutaneous, and cutaneomucous infections caused by Candida species, in capsules dosage form. Techniques by HPLC and UV first derivative spectrophotometry (UV-FDS) were selected for quantitative evaluation. In the development of release profile, several conditions were evaluated. Dissolution test parameters were considered appropriate when a most discriminative release profile for fluconazole capsules was yielded. Dissolution test conditions for fluconazole capsules were 900 mL of HCl 0.1 M, 37 ± 0.5 °C using baskets with 50 rpm for 30 min of test. The developed HPLC and UV-FDS methods for the antifungal evaluation were selective and met requirements for an appropriate and validated method, according to ICH and USP requirements. Both methods can be useful in the registration process of new drugs or their renewal. For routine analysis application cost, simplicity, equipment, solvents, speed, and application to large or small workloads should be observed.

scholarly journals Spectrophotometric determination of chlorpheniramine maleate and phenylpropanolamine hydrochloride in dosage forms

2013 ◽  
Vol 2 (5) ◽  
pp. 97-100 ◽  
Author(s):  
Arun Kaura ◽  
Vikas Gupta ◽  
G S Roy ◽  
Monika Kaura
Keyword(s):  
Pharmaceutical Journal ◽  
Dosage Forms ◽  
Uv Spectrophotometry ◽  
Chlorpheniramine Maleate ◽  
Simple Method ◽  
Pharmaceutical Dosage Forms ◽  
First Derivative ◽  
Phenylpropanolamine Hydrochloride ◽  
Derivative Uv Spectrophotometry

A rapid and simple method for simultaneous determination of Chlorpheniramine Maleate (CPM) and Phenylpropanolamine Hydrochloride (PPM) by first derivative UV spectrophotometry has been developed in combined pharmaceutical dosage forms. The proposed method was successfully applied for the determination of drugs in physical mixture and commercial formulations and results showed good linearity, precision and reproducibility. DOI: http://dx.doi.org/10.3329/icpj.v2i5.14436 International Current Pharmaceutical Journal, April 2013, 2(5): 97-100 

Sign in / Sign up

Export Citation Format

Share Document