One simple and sensitive procedure (simple spectrophotometric method) for the assay of drug clarithromycin in pure
form and formulations. This method involves the formation of ion-association complex between CAM and the TPOOO,
ARS and WFB. In order to establish the optimum conditions necessary for rapid and quantitative formation of coloured
product with maximum stability and sensitivity, the author performed experiments by measuring the absorbance at
λmax 480nm,420nm and580nm of respective series of solutions, varying one and fixing the other parameters in each
case such as type, volume and concentration of acid, organic solvent used for extraction, ratio of organic phase to
aqueous phase during extraction, shaking time and temperature. The variable parameters were optimized. The results
were statistically validated.