Effect of Extended Diapause on Evolution of Resistance to Transgenic Bacillus thuringiensis Corn by Northern Corn Rootworm (Coleoptera: Chrysomelidae)

2005 ◽  
Vol 98 (6) ◽  
pp. 2220-2234 ◽  
Author(s):  
P. D. Mitchell ◽  
D. W. Onstad
2015 ◽  
Vol 23 (6) ◽  
pp. 913-917 ◽  
Author(s):  
Isaac O. Oyediran ◽  
Phillip Matthews ◽  
Narendra Palekar ◽  
Wade French ◽  
Jared Conville ◽  
...  

2020 ◽  
Vol 45 (1) ◽  
Author(s):  
Jordan D Reinders ◽  
Zachary D Rystrom ◽  
Emily E Reinders ◽  
Timothy B Dang ◽  
Lance J Meinke

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Man P. Huynh ◽  
Chad Nielson ◽  
B. Wade French ◽  
Dalton C. Ludwick ◽  
Ryan W. Geisert ◽  
...  

AbstractThe northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation a year. When rearing the northern corn rootworm in the laboratory, in order to break diapause, it is necessary to expose eggs to a five month cold period before raising the temperature. By selective breeding of the small fraction of eggs that hatched without cold within 19–32 days post oviposition, we were able to develop a non-diapausing colony of the northern corn rootworm within five generations of selection. Through selection, the percentages of adult emergence from egg hatch without exposure to cold treatment significantly increased from 0.52% ± 0.07 at generation zero to 29.0% ± 2.47 at generation eight. During this process, we developed an improved method for laboratory rearing of both the newly developed non-diapausing strain as well as the diapausing strain. The development of the non-diapausing colony along with the improvements to the rearing system will allow researchers to produce up to six generations of the northern corn rootworm per year, which would facilitate research and advance our knowledge of this pest at an accelerated rate.


1998 ◽  
Vol 62 (3) ◽  
pp. 775-806 ◽  
Author(s):  
E. Schnepf ◽  
N. Crickmore ◽  
J. Van Rie ◽  
D. Lereclus ◽  
J. Baum ◽  
...  

SUMMARY During the past decade the pesticidal bacterium Bacillus thuringiensis has been the subject of intensive research. These efforts have yielded considerable data about the complex relationships between the structure, mechanism of action, and genetics of the organism’s pesticidal crystal proteins, and a coherent picture of these relationships is beginning to emerge. Other studies have focused on the ecological role of the B. thuringiensis crystal proteins, their performance in agricultural and other natural settings, and the evolution of resistance mechanisms in target pests. Armed with this knowledge base and with the tools of modern biotechnology, researchers are now reporting promising results in engineering more-useful toxins and formulations, in creating transgenic plants that express pesticidal activity, and in constructing integrated management strategies to insure that these products are utilized with maximum efficiency and benefit.


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