scholarly journals Corrigendum to: “Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays”

2020 ◽  
Vol 113 (5) ◽  
pp. 2568-2568
Keyword(s):  
Bacillus Thuringiensis ◽  
Laboratory Strain ◽  
Corn Rootworm ◽  
Single Plant ◽  
Diabrotica Barberi ◽  
Northern Corn Rootworm

Baseline Susceptibility of a Laboratory Strain of Northern Corn Rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) to Bacillus thuringiensis Traits in Seedling, Single Plant, and Diet-Toxicity Assays

2020 ◽  
Vol 113 (4) ◽  
pp. 1955-1962
Author(s):  
Adriano E Pereira ◽  
Man P Huynh ◽  
Amit Sethi ◽  
Ashley L Miles ◽  
B Wade French ◽  
...  
Keyword(s):  
Laboratory Strain ◽  
Dry Weight ◽  
Head Capsule ◽  
Corn Rootworm ◽  
Single Plant ◽  
Corn Hybrids ◽  
Diabrotica Barberi ◽  
Resistance Evolution ◽  
Plant Assays ◽  
Northern Corn Rootworm

Abstract The northern corn rootworm (NCR), Diabrotica barberi Smith & Lawrence, is an economic pest of maize in the U.S. Corn Belt. The objective of this study was to determine the baseline susceptibility of a laboratory NCR strain to Bt proteins eCry3.1Ab, mCry3A, Cry3Bb1, and Cry34/35Ab1 using seedling, single plant, and diet-toxicity assays. Plant assays were performed in greenhouse using corn hybrids expressing one of the Bt proteins and each respective near-isoline. Diet-toxicity assays, consisting of Bt proteins overlaid onto artificial diet were also conducted. In both plant assays, significantly more larvae survived Cry34/35Ab1-expressing corn compared with all other Bt-expressing corn, and larvae that survived eCry3.1Ab-expressing corn had significantly smaller head capsule widths compared with larvae that survived Cry34/35Ab1-expressing corn. In seedling assays, larvae surviving eCry3.1Ab-expressing corn also had significantly smaller head capsule widths compared with larvae that survived mCry3A-expressing corn. Additionally, larvae that survived mCry3A-expressing corn weighed significantly more than larvae surviving eCry3.1Ab- and Cry34/35Ab1-expressing corn. In single plant assays, no significant differences in larval dry weight was observed between any of the Bt-expressing corn. In diet assays, LC50s ranged from 0.14 (eCry3.1Ab) to 10.6 µg/cm2 (Cry34/35Ab1), EC50s ranged from 0.12 (Cry34/35Ab1) to 1.57 µg/cm2 (mCry3A), IC50s ranged from 0.08 (eCry3.1Ab) to 2.41 µg/cm2 (Cry34/35Ab1), and MIC50s ranged from 2.52 (eCry3.1Ab) to 14.2 µg/cm2 (mCry3A). These results establish the toxicity of four Bt proteins to a laboratory diapausing NCR strain established prior to the introduction of Bt traits and are important for monitoring resistance evolution in NCR field populations.

scholarly journals Susceptibility of northern corn rootworm Diabrotica barberi (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins

2015 ◽  
Vol 23 (6) ◽  
pp. 913-917 ◽  
Author(s):  
Isaac O. Oyediran ◽  
Phillip Matthews ◽  
Narendra Palekar ◽  
Wade French ◽  
Jared Conville ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Corn Rootworm ◽  
Diabrotica Barberi ◽  
Northern Corn Rootworm

scholarly journals Development of a nondiapausing strain of northern corn rootworm with rearing techniques for both diapausing and nondiapausing strains

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Man P. Huynh ◽  
Chad Nielson ◽  
B. Wade French ◽  
Dalton C. Ludwick ◽  
Ryan W. Geisert ◽  
...  
Keyword(s):  
Selective Breeding ◽  
Cold Treatment ◽  
Adult Emergence ◽  
Corn Rootworm ◽  
Improved Method ◽  
Egg Hatch ◽  
Diabrotica Barberi ◽  
Laboratory Rearing ◽  
Rearing Techniques ◽  
Northern Corn Rootworm

AbstractThe northern corn rootworm, Diabrotica barberi Smith & Lawrence, has a univoltine life cycle that typically produces one generation a year. When rearing the northern corn rootworm in the laboratory, in order to break diapause, it is necessary to expose eggs to a five month cold period before raising the temperature. By selective breeding of the small fraction of eggs that hatched without cold within 19–32 days post oviposition, we were able to develop a non-diapausing colony of the northern corn rootworm within five generations of selection. Through selection, the percentages of adult emergence from egg hatch without exposure to cold treatment significantly increased from 0.52% ± 0.07 at generation zero to 29.0% ± 2.47 at generation eight. During this process, we developed an improved method for laboratory rearing of both the newly developed non-diapausing strain as well as the diapausing strain. The development of the non-diapausing colony along with the improvements to the rearing system will allow researchers to produce up to six generations of the northern corn rootworm per year, which would facilitate research and advance our knowledge of this pest at an accelerated rate.

scholarly journals Partial characterization of Bt resistance and the bacteriome of western corn rootworm

2018 ◽  
Author(s):  
◽  
Dalton C. Ludwick
Keyword(s):  
New Technologies ◽  
Management Strategies ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Control Measures ◽  
Corn Rootworm ◽  
Diabrotica Barberi ◽  
The Usa ◽  
Diabrotica Virgifera Virgifera Leconte ◽  
Northern Corn Rootworm

Western corn rootworm (Diabrotica virgifera virgifera LeConte) and northern corn rootworm (Diabrotica barberi (Smith and Lawrence)) are major pests of maize in the USA. These pests have been managed with a variety of tactics over the last century. Both Diabrotica spp. have adapted to crop rotation in different ways in certain regions of the USA as well as to some of the insecticides targeted at them. D. v. virgifera has adapted to more of the chemical control measures and transgenic control methods. Discussed in this review are the challenges associated with managing both species, and how current management strategies might be combined and implemented to help manage damage from these species. Also, we discuss the potential for new technologies, such as RNA interference, to be used in the future.

scholarly journals Optimizing Egg Recovery From Wild Northern Corn Rootworm Beetles (Coleoptera: Chrysomelidae)

2019 ◽  
Vol 112 (6) ◽  
pp. 2737-2743
Author(s):  
Adriano E Pereira ◽  
Dalton C Ludwick ◽  
Julie Barry ◽  
Lance J Meinke ◽  
Daniel J Moellenbeck ◽  
...  
Keyword(s):  
Egg Production ◽  
Insect Pests ◽  
Corn Belt ◽  
Corn Rootworm ◽  
Cumulative Number ◽  
Diabrotica Barberi ◽  
Choice Tests ◽  
Field Season ◽  
Clear Plastic ◽  
Northern Corn Rootworm

Abstract The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), is one of the most important insect pests in the U.S. Corn Belt. Efforts to obtain eggs from wild northern corn rootworm populations using techniques developed for other rootworm species have been unsuccessful due to lack of oviposition. In 2016, we evaluated four oviposition media in choice tests within each of three female densities in 30.5 × 30.5 × 30.5 cm BugDorm cages. The number of eggs laid per female was significantly affected by female density and the interaction of female density × oviposition media, but oviposition was relatively poor in all oviposition media (1.2 eggs per female when averaging the three female densities and all oviposition media). Single females were also evaluated in nonchoice assays in 6 cm × 6 cm × 8 cm clear plastic boxes and averaged up to 108 eggs per female depending on the oviposition media. In 2017, the cumulative number of eggs laid per female in boxes with one female was not significantly different from the number of eggs laid per female in boxes with 3 females. In 2018, the cumulative number of eggs laid per female was not significantly different between female densities of 1, 3, 5, or 10 females per box. Total egg production per box therefore increased as female density increased. More than 27,000 wild northern corn rootworm eggs were collected from just 190 females when collected relatively early in the field season. We now have an efficient and robust system for obtaining eggs from wild northern corn rootworm females.

Response of northern corn rootworm,Diabrotica barberi Smith and Lawrence, to stereoisomers of 8-methyl-2-decyl propanoate

1985 ◽  
Vol 11 (1) ◽  
pp. 21-26 ◽  
Author(s):  
P. L. Guss ◽  
P. E. Sonnet ◽  
R. L. Carney ◽  
J. H. Tumlinson ◽  
P. J. Wilkin
Keyword(s):  
Corn Rootworm ◽  
Diabrotica Barberi ◽  
Northern Corn Rootworm
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