Carotenoid Production by Caterpillar Medicinal Mushrooms, Cordyceps militaris (Ascomycetes), under Different Culture Conditions

2020 ◽  
Vol 22 (12) ◽  
pp. 1191-1201
Author(s):  
Ying Yang ◽  
Ning Bu ◽  
Shenghou Wang ◽  
Jiaojiao Zhang ◽  
Ying Wang ◽  
...  
Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 461 ◽  
Author(s):  
Soo Kweon Lee ◽  
Ju Hun Lee ◽  
Hyeong Ryeol Kim ◽  
Youngsang Chun ◽  
Ja Hyun Lee ◽  
...  

Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.


2013 ◽  
Vol 796 ◽  
pp. 21-24
Author(s):  
Yu Qiao Jin ◽  
Bing Li

In order to study eri-silkworm Cordyceps militaris culture condition and the amino acid composition features in different parts of it, this experiment optimized eri-silkworm Cordyceps militaris artificial culture conditions, and determined the amino acid composition in different parts. The results showed that the inoculated eri-silkworm Cordyceps militaris which was kept at low temperature (18 ° C) and seventeen dark days grew well and began to use natural light in the eighteenth day with humidity 85%, temperature 21 ° C, which could induce the germination of the sporophore. The artificial cultivation period was about 40 days. Amino acid test shows that its sporophore and stroma are rich in 17 kinds of amino acids, including 7 kinds of essential amino acids. The higher levels of amino acids in its sporophore are lysine 15.28%, glutamate 15.05% and aspartate 7.55%. The higher levels of the amino acids in the stroma are glycine 16.11%, lysine 9.16% and glutamate 8.30%. The results indicate that eri-silkworm Cordyceps militaris can be artificially cultured. As there are differences of amino acid composition in different parts of eri-silkworm Cordyceps militaris, this study lays the foundation for its large-scale cultivation and the exploitation of its amino acid nutrition.


2019 ◽  
Vol 21 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Qianwang Zheng ◽  
Tao Wei ◽  
Yin Lin ◽  
Zhiwei Ye ◽  
Junfang Lin ◽  
...  

2017 ◽  
Vol 41 (2) ◽  
pp. 143-155 ◽  
Author(s):  
Ana S. Fernandes ◽  
Susana M. Paixão ◽  
Tiago P. Silva ◽  
José C. Roseiro ◽  
Luís Alves

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