MicroSAGE Analysis of 2,353 Expressed Genes in a Single Cell-Derived Colony of Undifferentiated Human Mesenchymal Stem Cells Reveals mRNAs of Multiple Cell Lineages

Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Since the transcription factor TWIST1 influences expansion of MSCs, its role in regulating cellular senescence was investigated. The present study demonstrated that silencing of TWIST1 in MSCs increased the occurrence of senescence, characterised by a SASP profile different from irradiation-induced senescent MSCs. Knowing that senescence alters cellular metabolism, cellular bioenergetics was monitored by using the Seahorse XF apparatus. Both TWIST1-silencing-induced and irradiation-induced senescent MSCs had a higher oxygen consumption rate compared to control MSCs, while TWIST1-silencing-induced senescent MSCs had a low extracellular acidification rate compared to irradiation-induced senescent MSCs. Overall, data indicated how TWIST1 regulation influenced senescence in MSCs and that TWIST1 silencing-induced senescence was characterised by a specific SASP profile and metabolic state.

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Early adhesion of human mesenchymal stem cells on TiO2 surfaces studied by single-cell force spectroscopy measurements

Journal of Molecular Recognition ◽

10.1002/jmr.2193 ◽

2012 ◽

Vol 25 (5) ◽

pp. 262-269 ◽

Cited By ~ 16

Author(s):

P. Bertoncini ◽

S. Le Chevalier ◽

S. Lavenus ◽

P. Layrolle ◽

G. Louarn

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Single Cell ◽

Human Mesenchymal Stem Cells ◽

Force Spectroscopy ◽

Cell Force

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Mapping SOX9 transcriptional dynamics during multi-lineage differentiation of human mesenchymal stem cells

10.1101/2021.11.29.470107 ◽

2021 ◽

Author(s):

Kannan Govindaraj ◽

Sakshi Khurana ◽

Marcel Karperien ◽

Janine Nicole Post

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Transcription Factor ◽

Single Cell ◽

Adipogenic Differentiation ◽

Human Mesenchymal Stem Cells ◽

Cell Types ◽

Differentiation Potential ◽

Lineage Differentiation ◽

Transcriptional Dynamics

The master transcription factor SOX9 is a key player during chondrocyte differentiation, cartilage development, homeostasis and disease. Modulation of SOX9 and its target gene expression is essential during chondrogenic, osteogenic and adipogenic differentiation of human mesenchymal stem cells (hMSCs). However, lack of sufficient knowledge about the signaling interplay during differentiation remains one of the main reasons preventing successful application of hMSCs in regenerative medicine. We previously showed that Transcription Factor - Fluorescence Recovery After Photobleaching (TF-FRAP) can be used to study SOX9 dynamics at the single cell level. We showed that changes in SOX9 dynamics are linked to its transcriptional activity. Here, we investigated SOX9 dynamics during differentiation of hMSCs into the chondrogenic, osteogenic and adipogenic lineages. We show that there are clusters of cells in hMSCs with distinct SOX9 dynamics, indicating that there are a number of subpopulations present in the heterogeneous hMSCs. SOX9 dynamics data at the single cell resolution revealed novel insights about its activity in these subpopulations (cell types). In addition, the response of SOX9 to differentiation stimuli varied in these subpopulations. Moreover, we identified donor specific differences in the number of cells per cluster in undifferentiated hMSCs, and this correlated to their differentiation potential.

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Dynamic Imaging of Marrow-Resident Granulocytes Interacting with Human Mesenchymal Stem Cells upon Systemic Lipopolysaccharide Challenge

Stem Cells International ◽

10.1155/2013/656839 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Jay T. Myers ◽

Deborah S. Barkauskas ◽

Alex Y. Huang

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Single Cell ◽

Human Mesenchymal Stem Cells ◽

Dynamic Imaging ◽

Whole Body ◽

Whole Body Imaging ◽

Single Cell Level ◽

Cell Level

Human mesenchymal stem cells (hMSCs) have gained intense research interest due to their immune-modulatory, tissue differentiating, and homing properties to sites of inflammation. Despite evidence demonstrating the biodistribution of infused hMSCs in target organs using static fluorescence imaging or whole-body imaging techniques, surprisingly little is known about how hMSCs behave dynamically within host tissues on a single-cell levelin vivo. Here, we infused fluorescently labeled clinical-grade hMSCs into immune-competent mice in which neutrophils and monocytes express a second fluorescent marker under the lysozyme M (LysM) promoter. Using intravital two-photon microscopy (TPM), we were able for the first time to capture dynamic interactions between hMSCs and LysM+granulocytes in the calvarium bone marrow of recipient mice during systemic LPS challenge in real time. Interestingly, many of the infused hMSCs remained intact despite repeated cellular contacts with host neutrophils. However, we were able to observe the destruction and subsequent phagocytosis of some hMSCs by surrounding granulocytes. Thus, our imaging platform provides opportunities to gain insight into the biology and therapeutic mechanisms of hMSCsin vivoat a single-cell level within live hosts.

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The time course of engraftment of human mesenchymal stem cells in fetal heart demonstrates that Purkinje fiber aggregates derive from a single cell and not multi-cell homing

Experimental Hematology ◽

10.1016/j.exphem.2006.04.019 ◽

2006 ◽

Vol 34 (7) ◽

pp. 926-933 ◽

Cited By ~ 10

Author(s):

Evan J. Colletti ◽

Graça Almeida-Porada ◽

Jason Chamberlain ◽

Esmail D. Zanjani ◽

Judith A. Airey

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Single Cell ◽

Time Course ◽

Fetal Heart ◽

Human Mesenchymal Stem Cells ◽

Purkinje Fiber ◽

Cell Homing

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Bovine tongue epithelium-derived cells: A new source of bovine mesenchymal stem cells

Bioscience Reports ◽

10.1042/bsr20181829 ◽

2020 ◽

Vol 40 (4) ◽

Author(s):

Jienny Lee ◽

Jeong Su Byeon ◽

Na-Yeon Gu ◽

Siu Lee ◽

Se-A Lee ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Culture Conditions ◽

Class I Mhc ◽

Mhc I ◽

Cell Lineages ◽

Mhc Ii ◽

Multiple Cell ◽

Low Glucose

Abstract Mesenchymal stem cells (MSCs) possess the ability to differentiate into multiple cell lineages, and thus, confer great potential for use in regenerative medicine and biotechnology. In the present study, we attempted to isolate and characterize bovine tongue tissue epithelium-derived MSCs (boT-MSCs) and investigate the culture conditions required for long-term culturing of boT-MSCs. boT-MSCs were successfully isolated by the collagenase digestion method and their proliferative capacity was maintained for up to 20 or more passages. We observed a significant increase in the proliferation of boT-MSCs during the 20 consecutive passages under low-glucose Dulbecco’s modified Eagle’s medium culture condition among the three culture conditions. These boT-MSCs presented pluripotency markers (octamer-binding transcription factor 3/4 (Oct3/4) and sex determining region Y-box2 (Sox2)) and cell surface markers, which included CD13, CD29, CD44, CD73, CD90, CD105, CD166, and major histocompatibility complex (MHC) class I (MHC-I) but not CD11b, CD14, CD31, CD34, CD45, CD80, CD86, CD106, CD117, and MHC-II at third passage. Moreover, these boT-MSCs could differentiate into mesodermal (adipocyte, osteocyte, and chondrocyte) cell lineages. Thus, the present study suggests that the tongue of bovines could be used as a source of bovine MSCs.

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