scholarly journals Genome-Wide Reprogramming in Hybrids of Somatic Cells and Embryonic Stem Cells

Stem Cells ◽  
2007 ◽  
Vol 25 (5) ◽  
pp. 1104-1113 ◽  
Author(s):  
Dominic J. Ambrosi ◽  
Borko Tanasijevic ◽  
Anupinder Kaur ◽  
Craig Obergfell ◽  
Rachel J. O'Neill ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Genome Wide

Protecting genomic integrity in somatic cells and embryonic stem cells

2007 ◽  
Vol 614 (1-2) ◽  
pp. 48-55 ◽  
Author(s):  
Y. Hong ◽  
R.B. Cervantes ◽  
E. Tichy ◽  
J.A. Tischfield ◽  
P.J. Stambrook
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Genomic Integrity

scholarly journals Genome-wide Kinase-Chromatin Interactions Reveal the Regulatory Network of ERK Signaling in Human Embryonic Stem Cells

2013 ◽  
Vol 50 (6) ◽  
pp. 844-855 ◽  
Author(s):  
Jonathan Göke ◽  
Yun-Shen Chan ◽  
Junli Yan ◽  
Martin Vingron ◽  
Huck-Hui Ng
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Regulatory Network ◽  
Embryonic Stem ◽  
Erk Signaling ◽  
Chromatin Interactions ◽  
Genome Wide

scholarly journals Hybridization of Testis-Derived Stem Cells with Somatic Cells and Embryonic Stem Cells in Mice1

2012 ◽  
Vol 86 (6) ◽  
Author(s):  
Masanori Takehashi ◽  
Masako Tada ◽  
Mito Kanatsu-Shinohara ◽  
Hiroko Morimoto ◽  
Yasuhiro Kazuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem

scholarly journals Genome-Wide Definition of Promoter and Enhancer Usage during Neural Induction of Human Embryonic Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126590 ◽  
Author(s):  
Valentina Poletti ◽  
Alessia Delli Carri ◽  
Guidantonio Malagoli Tagliazucchi ◽  
Andrea Faedo ◽  
Luca Petiti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Neural Induction ◽  
Genome Wide ◽  
Wide Definition ◽  
Definition Of

scholarly journals Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells

Nature ◽  
2011 ◽  
Vol 473 (7347) ◽  
pp. 394-397 ◽  
Author(s):  
William A. Pastor ◽  
Utz J. Pape ◽  
Yun Huang ◽  
Hope R. Henderson ◽  
Ryan Lister ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Genome Wide

scholarly journals Induction of Recurrent Break Cluster Genes in Neural Progenitor Cells Differentiated from Embryonic Stem Cells In Culture

2019 ◽  
Author(s):  
Aseda Tena ◽  
Yuxiang Zhang ◽  
Nia Kyritsis ◽  
Anne Devorak ◽  
Jeffrey Zurita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Es Cells ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
Neural Genes ◽  
Genome Wide ◽  
Primary Mouse

ABSTRACTMild replication stress enhances appearance of dozens of robust recurrent genomic break clusters, termed RDCs, in cultured primary mouse neural stem and progenitor cells (NSPCs). Robust RDCs occur within genes (“RDC-genes”) that are long and have roles in neural cell communications and/or have been implicated in neuropsychiatric diseases or cancer. We sought to develop an in vitro approach to determine whether specific RDC formation is associated with neural development. For this purpose, we adapted a system to induce neural progenitor cell (NPC) development from mouse embryonic stem cell (ESC) lines deficient for XRCC4 plus p53, a genotype that enhances DNA double-strand break (DSB) persistence to enhance detection. We tested for RDCs by our genome wide DSB identification approach that captures DSBs genome-wide via their ability to join to specific genomic Cas9/sgRNA-generated bait DSBs. In XRCC4/p53-deficient ES cells, we detected 7 RDCs, which were in genes, with two RDCs being robust. In contrast, in NPCs derived from these ES cell lines, we detected 29 RDCs, a large fraction of which were robust and associated with long, transcribed neural genes that were also robust RDC-genes in primary NSPCs. These studies suggest that many RDCs present in NSPCs are developmentally influenced to occur in this cell type and indicate that induced development of NPCs from ES cells provides an approach to rapidly elucidate mechanistic aspects of NPC RDC formation.SIGNIFICANCE STATEMENTWe previously discovered a set of long neural genes susceptible to frequent DNA breaks in primary mouse brain progenitor cells. We termed these genes RDC-genes. RDC-gene breakage during brain development might alter neural gene function and contribute to neurological diseases and brain cancer. To provide an approach to characterize the unknown mechanism of neural RDC-gene breakage, we asked whether RDC-genes appear in neural progenitors differentiated from embryonic stem cells in culture. Indeed, robust RDC-genes appeared in neural progenitors differentiated in culture and many overlapped with robust RDC-genes in primary brain progenitors. These studies indicate that in vitro development of neural progenitors provides a model system for elucidating how RDC-genes are formed.

scholarly journals A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation

2011 ◽  
Vol 195 (6) ◽  
pp. i9-i9 ◽  
Author(s):  
Bart A. Westerman ◽  
A. Koen Braat ◽  
Nicole Taub ◽  
Marko Potman ◽  
Joseph H.A. Vissers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Rnai Screen ◽  
Genome Wide ◽  
A Genome

scholarly journals NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Susan L. Kloet ◽  
Ino D. Karemaker ◽  
Lisa van Voorthuijsen ◽  
Rik G. H. Lindeboom ◽  
Marijke P. Baltissen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Interacting Protein ◽  
Genome Wide

Effect of BMP4 preceded by retinoic acid and co-culturing ovarian somatic cells on differentiation of mouse embryonic stem cells into oocyte-like cells

2015 ◽  
Vol 57 (5) ◽  
pp. 378-388 ◽  
Author(s):  
Soghra Bahmanpour ◽  
Nehleh Zarei Fard ◽  
Tahereh Talaei-Khozani ◽  
Ahmad Hosseini ◽  
Tahereh Esmaeilpour
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells
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