Molecular Detection of a Novel Fowl Adenovirus Serotype-4 (FadV-4) from an Outbreak of Hepatitis Hydropericardium Syndrome in Commercial Broiler Chickens in Egypt

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Hesham Sultan ◽  
Abd-Elsatar Arafa ◽  
Amany Adel ◽  
Karim Selim ◽  
Mohamed Hossiny ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Molecular Detection ◽  
Adenovirus Serotype ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Commercial Broiler ◽  
Fowl Adenovirus Serotype 4

scholarly journals Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jingyi Liu ◽  
Nan Mei ◽  
Yaling Wang ◽  
Xinjin Shi ◽  
Hongjun Chen
Keyword(s):  
Amino Acid Sequence Analysis ◽  
Serum Samples ◽  
Protein Amino Acid ◽  
Adenovirus Serotype ◽  
Linear Peptide ◽  
Hydropericardium Syndrome ◽  
Hexon Protein ◽  
Fowl Adenovirus ◽  
Fowl Adenovirus Serotype 4

AbstractFowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.

scholarly journals Pathogenesis of Hypervirulent Fowl Adenovirus Serotype 4: The Contributions of Viral and Host Factors

Viruses ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 741 ◽  
Author(s):  
Zeng Wang ◽  
Jun Zhao
Keyword(s):  
Host Defense ◽  
Host Factors ◽  
Present Review ◽  
Defense System ◽  
Adenovirus Serotype ◽  
Vaccine Candidates ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Host Defense System ◽  
Fowl Adenovirus Serotype 4

Since 2015, severe outbreaks of hepatitis-hydropericardium syndrome (HHS), caused by hypervirulent fowl adenovirus serotype 4 (FAdV-4), have emerged in several provinces in China, posing a great threat to poultry industry. So far, factors contributing to the pathogenesis of hypervirulent FAdV-4 have not been fully uncovered. Elucidation of the pathogenesis of FAdV-4 will facilitate the development of effective FAdV-4 vaccine candidates for the control of HHS and vaccine vector. The interaction between pathogen and host defense system determines the pathogenicity of the pathogen. Therefore, the present review highlights the knowledge of both viral and host factors contributing to the pathogenesis of hypervirulent FAdV-4 strains to facilitate the related further studies.

scholarly journals Hydropericardium syndrome caused by fowl adenovirus serotype 4 in replacement pullets

2019 ◽  
Vol 81 (2) ◽  
pp. 245-251 ◽  
Author(s):  
Zhen CHEN ◽  
Shaohua SHI ◽  
Baoming QI ◽  
Su LIN ◽  
Cuiteng CHEN ◽  
...  
Keyword(s):  
Adenovirus Serotype ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Fowl Adenovirus Serotype 4

scholarly journals Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 107 ◽  
Author(s):  
Junfeng Gao ◽  
Mingliang Zhao ◽  
Xueyan Duan ◽  
Yongqiang Wang ◽  
Hong Cao ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Ectopic Expression ◽  
Cellular Protein ◽  
Economic Losses ◽  
Adenovirus Serotype ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Infected Cells ◽  
Lmh Cells ◽  
Fowl Adenovirus Serotype 4

Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS), leading to severe economic losses in the poultry industry. Although the pathogenesis of FAdV-4 infection has caused much attention, the underlying molecular mechanisms remain poorly understood. Here, we identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the FAdV-4 capsid protein hexon. We found that ectopic expression of CCT7 in leghorn male hepatocellular (LMH) cells enhanced hexon expression in pRK5-flag-hexon transfected cells. On the contrary, knockdown of cellular CCT7 by RNAi markedly reduced hexon expression in FAdV-4-infected cells and suppressed viral replication. These data suggest that CCT7 is required for FAdV-4 replication and may serve as a potential target for controlling FAdV-4 infection.

scholarly journals Corrigendum to Hydropericardium syndrome outbreak caused by fowl adenovirus serotype 4 in China in 2015

2016 ◽  
Vol 97 (10) ◽  
pp. 2797-2797 ◽  
Author(s):  
Yu-juan Niu ◽  
Wei Sun ◽  
Gui-hua Zhang ◽  
Ya-jin Qu ◽  
Peng-fei Wang ◽  
...  
Keyword(s):  
Adenovirus Serotype ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Fowl Adenovirus Serotype 4
Sign in / Sign up

Export Citation Format

Share Document