FSH receptor gene expression during ovarian follicle development in sheep

1995 ◽  
Vol 15 (3) ◽  
pp. 273-281 ◽  
Author(s):  
D J Tisdall ◽  
K Watanabe ◽  
N L Hudson ◽  
P Smith ◽  
K P McNatty
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Ovarian Follicle ◽  
Receptor Gene ◽  
Fsh Receptor ◽  
Follicular Growth ◽  
Fetal Sheep ◽  
Adult Sheep ◽  
Receptor Gene Expression ◽  
Fsh Receptor Gene

ABSTRACT A key question in elucidating the role of FSH in ovarian function is to determine when during follicular growth the FSH receptor first appears. The aim of this study was to examine the site and time of FSH receptor gene expression during early follicular growth. This study was carried out on ovaries of adult sheep during the luteal and prostaglandin-induced follicular phase of the oestrous cycle and also on ovaries of fetal sheep at 90, 100, 120 and 135 days of gestation (term=day 147). Using reverse transcription-PCR and a set of PCR primers spanning exons 8/9/10, two partial FSH receptor cDNAs (500 and 310 bp) were isolated from adult sheep ovary. It was shown by sequencing that exon 8 was deleted in the 310 bp cDNA, implying that this was part of an alternatively spliced FSH receptor transcript. Using RNA in situ hybridisation on ovaries of adult sheep, FSH receptor mRNA was observed in granulosa cells of early preantral follicles with one to two cell layers and it was seen that gene expression continued throughout folliculogenesis into advanced stages of atresia. Moreover, in the fetus, FSH receptor gene expression was detected in follicles with two or more layers of granulosa cells in ovaries taken at 100, 120 and 135 days of gestation. These results suggest that the FSH receptor gene is expressed after the granulosa cells of a folllicle have begun to divide but not during the earliest stages of follicle growth, namely the transformation of a primordial follicle to a primary follicle.

Control of low density lipoprotein receptor gene expression in steroidogenic cells

1989 ◽  
Vol 67 (8) ◽  
pp. 968-973 ◽  
Author(s):  
Koichiro Takagi ◽  
Jerome F. Strauss III
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Granulosa Cells ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Low Density ◽  
Steroidogenic Cells ◽  
Receptor Gene Expression

Low density lipoprotein (LDL)-carried cholesterol is a primary substrate for steroid hormone synthesis by luteinized human granulosa cells. Chorionic gonadotropin and 8-bromo-cAMP both increase LDL receptor levels in granulosa cells by stimulating accumulation of the receptor mRNA. LDL and 25-hydroxycholesterol reduce LDL receptor expression, but this suppressive effect is partially overcome by 8-bromo-cAMP. Using fusion gene constructs containing the LDL receptor gene promoter transfected into JEG-3 cells, a cyclic AMP responsive enhancer could not be identified in the LDL receptor gene upstream promoter in transfection studies. We suggest that the LDL receptor gene in human steroidogenic cells is under negative control by a sterol effector, but that a cyclic AMP triggered process overcomes, to some extent, the sterol-mediated suppression. The detailed mechanisms by which sterol and cyclic AMP modulate LDL receptor gene expression remain to be elucidated.Key words: low density lipoproteins, low density lipoprotein receptors, cholesterol, steroidogenesis, gonadotropins.

Luteinizing Hormone Induces Progesterone Receptor Gene Expression in Cultured Porcine Granulosa Cells

Endocrinology ◽  
1991 ◽  
Vol 129 (3) ◽  
pp. 1621-1627 ◽  
Author(s):  
MASAZUMI IWAI ◽  
KEIKO YASUDA ◽  
MASATSUNE FUKUOKA ◽  
TOSHIKO IWAI ◽  
KENJI TAKAKURA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Luteinizing Hormone ◽  
Progesterone Receptor ◽  
Granulosa Cells ◽  
Receptor Gene ◽  
Porcine Granulosa Cells ◽  
Progesterone Receptor Gene ◽  
Receptor Gene Expression

scholarly journals Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells

2011 ◽  
Vol 23 (2) ◽  
pp. 196-203 ◽  
Author(s):  
Verena Nordhoff ◽  
Barbara Sonntag ◽  
Dominik von Tils ◽  
Martin Götte ◽  
Andreas N. Schüring ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Granulosa Cells ◽  
Receptor Gene ◽  
Fsh Receptor ◽  
Steroid Production ◽  
Human Granulosa Cells ◽  
Receptor Gene Polymorphism ◽  
Fsh Receptor Gene

Expression of the ovine stem cell factor gene during folliculogenesis in late fetal and adult ovaries

1997 ◽  
Vol 18 (2) ◽  
pp. 127-135 ◽  
Author(s):  
D J Tisdall ◽  
L D Quirke ◽  
P Smith ◽  
K P McNatty
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Granulosa Cells ◽  
Stem Cell Factor ◽  
Ovarian Follicle ◽  
Northern Analysis ◽  
Nucleotide Sequencing ◽  
Sequence Difference ◽  
Follicle Growth ◽  
Fetal Sheep

ABSTRACT Two ovine stem cell factor (oSCF) cDNAs (822 bp and 738 bp) were generated from ovarian follicle mRNA by RT-PCR. Nucleotide sequencing revealed that the oSCF 822 bp cDNA encodes a precursor protein of 274 amino acids. An amino acid change 109E to 109Q was the only sequence difference from that previously described for this species. The smaller (738 bp) oSCF cDNA was shown by nucleotide sequencing to be an mRNA splice variant, equivalent to that found in other mammals, in which an exon (84 bp) encoding a potential proteolytic cleavage site is removed. Northern analysis revealed a single transcript of approximately 6·5 kb in follicles, corpora lutea and stroma of mid-luteal sheep ovaries. In situ hybridization was used to detect oSCF mRNA within ovaries of fetal sheep on days 90, 100, 120 and 135 of gestation (term=147) and of adult sheep within the breeding season. In fetal and adult ovaries, oSCF mRNA was detected in the granulosa cells of follicles at all stages of follicle growth (primordial through to antral). The SCF gene was also expressed in granulosa cells of atretic follicles but appeared to be down-regulated in the cumulus cells surrounding the oocyte at more advanced stages of atresia. In fetal ovaries at day 90 of gestation (90DG), oSCF was expressed in the subepithelial mesenchymal cells of the ovarian cortex. By 100DG the gene expression in the subepithelial cells became restricted to a narrow region below the epithelium, and areas of expression were observed in groups of cells around isolated oocytes, primordial and primary follicles. oSCF gene expression also occurred in the surface epithelial cells of 90DG ovaries, the expression was absent from these cells by 135DG and in adult ovaries. Localization of oSCF mRNA was observed in the ovarian rete and endothelial cells of blood vessels of fetal ovaries. These results suggest that oSCF may have an important and continuous role in the development and/or maintenance of germ cells during follicle growth and atresia in sheep.

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