scholarly journals A novel oestrogen-regulated gene in human breast cancer cells identified by differential display

1998 ◽  
Vol 20 (3) ◽  
pp. 375-380 ◽  
Author(s):  
R Stephen ◽  
D Corcoran ◽  
PD Darbre
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Differential Display ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Northern Blotting ◽  
Breast Cancer Cell Lines ◽  
Human Breast Cancer Cells ◽  
Human Breast

Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859 of the published sequence for the mRNA for human megakaryocyte CD63 antigen but has a 3' tail extended by 23 nucleotides. Northern blotting has confirmed that this mRNA is regulated by oestrogen in both MCF7 and T47D human breast cancer cell lines.

scholarly journals Anthocyanins Isolated from Vitis coignetiae Pulliat Enhances Cisplatin Sensitivity in MCF-7 Human Breast Cancer Cells through Inhibition of Akt and NF-κB Activation

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3623 ◽  
Author(s):  
Anjugam Paramanantham ◽  
Min Jeong Kim ◽  
Eun Joo Jung ◽  
Hye Jung Kim ◽  
Seong-Hwan Chang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Breast Cancer Cell Lines ◽  
Cell Phenotype ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Anti Cancer ◽  
Mcf 7

Anthocyanins isolated from Vitis coignetiae Pulliat (Meoru in Korea) (AIMs) have various anti-cancer properties by inhibiting Akt and NF-κB which are involved in drug resistance. Cisplatin (CDDP) is one of the popular anti-cancer agents. Studies reported that MCF-7 human breast cancer cells have high resistance to CDDP compared to other breast cancer cell lines. In this study, we confirmed CDDP resistance of MCF-7 cells and tested whether AIMs can overcome CDDP resistance of MCF-7 cells. Cell viability assay revealed that MCF-7 cells were more resistant to CDDP treatment than MDA-MB-231 breast cancer cells exhibiting aggressive and high cancer stem cell phenotype. AIMs significantly augmented the efficacy of CDDP with synergistic effects on MCF-7 cells. Molecularly, Western blot analysis revealed that CDDP strongly increased Akt and moderately reduced p-NF-κB and p-IκB and that AIMs inhibited CDDP-induced Akt activation, and augmented CDDP-induced reduction of p-NF-κB and p-IκB in MCF-7 cells. In addition, AIMs significantly downregulated an anti-apoptotic protein, XIAP, and augmented PARP-1 cleavage in CDDP-treated MCF-7 cells. Moreover, under TNF-α treatment, AIMs augmented CDDP efficacy with inhibition of NF-κB activation on MCF-7 cells. In conclusion, AIMs enhanced CDDP sensitivity by inhibiting Akt and NF-κB activity of MCF-7 cells that show relative intrinsic CDDP resistance.

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

2014 ◽  
Vol 9 (6) ◽  
pp. 647-658 ◽  
Author(s):  
Urszula Lewandowska ◽  
Katarzyna Owczarek ◽  
Karolina Szewczyk ◽  
Dorota Sosnowska ◽  
Maria Koziołkiewicz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Breast Cancer Cell Lines ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Evening Primrose ◽  
Invasive Human Breast Cancer ◽  
Mcf 7

AbstractThis study examines some of the biological activities of an evening primrose flavanol preparation (EPFP) against non-invasive human breast cancer cells (MCF-7). The results are compared with those obtained for highly invasive human breast cancer cells (MDAMB-231). The results show, for the first time, that EPFP reduces MCF-7 cell number, IC50 = 75 µM gallic acid equivalents/GAE for 72 h incubation, and reduces migration to 52% of the control value at 100 µM L−1 GAE. EPFP caused favorable changes in Bcl-2/Bax mRNA ratio, which rendered MCF-7 cells more sensitive to apoptosis: the number of apoptotic cells increased 2.2-fold vs. control at 100 µM GAE. Furthermore, 100 µ M L−1 GAE EPFP caused a 1.8-fold reduction in the activity of metalloproteinase-9 (MMP-9) secreted to the culture medium by MCF-7 cells. Moreover, EPFP suppressed the expression of selected genes of matrix metalloproteinases (MMPs), a proliferation marker (Ki67), and vascular endothelial growth factor (VEGF). In conclusion, the results of this study suggest that EPFP may exhibit proapoptotic, antiproliferative, antimigratory, and antimetastatic potential towards both selected human breast cancer cell lines, which is more pronounced in the case of the highly invasive MDA-MB-231 cells.

scholarly journals Long-term dexamethasone treatment increases the engraftment efficiency of human breast cancer cells in adult zebrafish

2021 ◽  
Vol 2 ◽  
pp. 100007
Author(s):  
Juliana Moreira Mendonça-Gomes ◽  
Thalita Marcolan Valverde ◽  
Thaís Maria da Mata Martins ◽  
Ives Charlie-Silva ◽  
Barbara Nunes Padovani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Adult Zebrafish ◽  
Human Breast ◽  
Dexamethasone Treatment
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