BIOLOGICAL AND IMMUNOLOGICAL PROPERTIES OF HUMAN PITUITARY FOLLICLE STIMULATING HORMONE OBTAINED BY STARCH GEL ELECTROPHORESIS

1963 ◽  
Vol 25 (4) ◽  
pp. 541-547 ◽  
Author(s):  
W. R. BUTT ◽  
A. C. CROOKE ◽  
F. J. CUNNINGHAM ◽  
ANNELIESE WOLF
Keyword(s):  
Follicle Stimulating Hormone ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Starch Gel Electrophoresis ◽  
Minimum Effective Dose ◽  
Hormone Activity ◽  
Human Pituitary ◽  
Main Fraction ◽  
Starch Gel ◽  
Stimulating Hormone

SUMMARY A highly potent preparation of human follicle stimulating hormone (FSH) was submitted to electrophoresis in starch gel. A fraction containing luteinizing hormone activity was separated from the main fraction of FSH. The latter showed no luteinizing activity by the ovarian ascorbic acid depletion method with 125 times the minimum effective dose in the assay for FSH. Synergistic joint action (see p. 544) was shown between the two fractions. An antiserum raised to the follicle stimulating component was investigated by red cell haemagglutination-inhibition tests. Its titre was only slightly reduced after absorption with chorionic gonadotrophin and haemagglutination was inhibited by preparations of FSH containing 0·5 μg./ml. (1·5 mg. HMG 24/ml.), but not by solutions of chorionic gonadotrophin containing up to 100 i.u./ml. This is regarded as evidence that the antiserum is fairly specific for FSH.

Therapeutic use of gonadotrophins and clomiphene

1969 ◽  
Vol 7 (9) ◽  
pp. 33-35
Keyword(s):  
Pregnant Women ◽  
Follicle Stimulating Hormone ◽  
Therapeutic Use ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Synthetic Compound ◽  
Pituitary Glands ◽  
Post Menopausal ◽  
Stimulating Hormone

The three substances now used to stimulate the gonads in infertility are human follicle stimulating hormone (HFSH) obtained mainly from post-menopausal urine, but also from human pituitary glands, human chorionic gonadotrophin (HCG) extracted from the urine of pregnant women, and clomiphene (Clomid - Merrell), a synthetic compound which we reviewed in 1967.1

OBSERVATIONS ON THE ASSAY OF HUMAN URINARY FOLLICLE-STIMULATING HORMONE BY THE AUGMENTATION TEST IN MICE

1966 ◽  
Vol 35 (2) ◽  
pp. 199-206 ◽  
Author(s):  
P. S. BROWN ◽  
M. WELLS
Keyword(s):  
No Value ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
High Potency ◽  
Human Pituitary ◽  
Ovarian Weight ◽  
C3h Mice ◽  
C57bl Mice ◽  
Stimulating Hormone

SUMMARY The follicle-stimulating hormone (FSH) content of urinary gonadotrophic extracts was assayed by its effect on the ovarian weight of immature mice when given in conjunction with 40 i.u. human chorionic gonadotrophin. About three-quarters of all routine assays gave values of λ between 0·15 and 0·30. Precision was slightly increased when the material was given in three rather than in five injections. Correction of ovarian weight for body weight was either invalid or of no value in reducing variance. Removal of between-litter variance increased precision considerably. Mice of three randomly bred colonies were all satisfactory, and inbred C57BL mice were also suitable for the assay. C3H mice were less sensitive. The efficiency of different methods of extracting FSH from urine was examined. The method of Johnsen (1958) using precipitation with tannic acid was considered the most satisfactory and gave extracts of high potency and low bulk. Limited experiments in which purified human pituitary FSH was assayed with and without added luteinizing hormone, gave results compatible with the assumption that the method is specific for FSH.

PURIFICATION OF FOLLICLE-STIMULATING HORMONE FROM HUMAN ANTERIOR PITUITARY GLANDS

1964 ◽  
Vol 42 (6) ◽  
pp. 841-849 ◽  
Author(s):  
W. H. McShan ◽  
B. B. Saxena ◽  
R. O. Creek
Keyword(s):  
Gel Electrophoresis ◽  
Anterior Pituitary ◽  
Follicle Stimulating Hormone ◽  
Starch Gel Electrophoresis ◽  
Thyrotropic Hormone ◽  
Pituitary Glands ◽  
Starch Gel ◽  
Gonadotropic Activity ◽  
Human Anterior Pituitary ◽  
Stimulating Hormone

The results of this study indicate that highly purified follicle-stimulating hormone (FSH) was prepared from human anterior pituitary glands by ammonium sulphate (AS) fractionation, zone electrophoresis, and starch gel electrophoresis. The activity of this preparation was approximately 14.7 times that of the sheep pituitary FSH standard. The fractions from zone and starch gel electrophoresis with which luteinizing hormone (LH) was associated also contained thyrotropic hormone (TSH). There was little decrease in the gonadotropic activity of human anterior pituitary glands recovered at different times up to 24 hours post-mortem.

THE EFFECT OF HUMAN GONADOTROPHINS ON A PATIENT WITH SIMMONDS'S DISEASE

1964 ◽  
Vol 46 (2) ◽  
pp. 292-299 ◽  
Author(s):  
A. C. Crooke ◽  
W. R. Butt ◽  
R. F. Palmer ◽  
R. Morris ◽  
D. B. Morgan
Keyword(s):  
Considerable Increase ◽  
Follicle Stimulating Hormone ◽  
Follicular Development ◽  
Pituitary Tumour ◽  
Chorionic Gonadotrophin ◽  
Surgical Removal ◽  
Human Pituitary ◽  
Timing Of Treatment ◽  
Stimulating Hormone

ABSTRACT A woman with Simmonds's disease which followed surgical removal of a pituitary tumour was treated with human pituitary follicle stimulating hormone and chorionic gonadotrophin. The treatment had no effect on her metabolic balances of nitrogen or electrolytes but caused a considerable increase in her excretion of oestriol with relatively little change in pregnanediol, a pattern associated with follicular development without ovulation. Changes in the timing of treatment with gonadotrophins and the addition of other hormones failed to alter this pattern, the nature and causes of which are discussed.

STUDIES ON HUMAN CHORIONIC GONADOTROPHIN

1968 ◽  
Vol 59 (1) ◽  
pp. 120-138 ◽  
Author(s):  
A. H. W. M. Schuurs ◽  
E. de Jager ◽  
J. D. H. Homan
Keyword(s):  
Gel Electrophoresis ◽  
Complement Fixation ◽  
Haemagglutination Inhibition ◽  
Fundamental Difference ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Starch Gel Electrophoresis ◽  
International Standard ◽  
Electrophoretic Mobilities ◽  
Starch Gel

ABSTRACT Preparations of Human Chorionic Gonadotrophin (HCG) with varying degrees of homogeneity were investigated immunochemically. The preparations were compared qualitatively by combined starch gel electrophoresis and immunoelectrophoresis, by immunodiffusion and immunoelectrophoresis in agar, and, quantitatively by haemagglutination inhibition and by complement fixation reactions. The components present in pure HCG preparations which differed in electrophoretic mobilities, in N-acetyl neuraminic acid (NANA) content and in biological potencies, appeared to be immunochemically identical. This implies that results of immunochemical and biological estimations need not correlate with each other. This finding is compared with relevant data in the literature. It is proposed to use the 2nd International Standard for HCG for both immunochemical and biological estimations, but, because of the fundamental difference between the results of the two types of estimations, to express the immunochemically determined values in »International Immunochemical Units« (IIU).

scholarly journals Effect of Human Pituitary-follicle-stimulating Hormone and Chorionic Gonadotrophin in Stein-Leventhal Syndrome

BMJ ◽  
1963 ◽  
Vol 1 (5338) ◽  
pp. 1119-1123 ◽  
Author(s):  
A. C. Crooke ◽  
W. R. Butt ◽  
R. Palmer ◽  
R. Morris ◽  
R. L. Edwards ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Stimulating Hormone

PREGNANCY FOLLOWING TREATMENT WITH HUMAN PITUITARY FOLLICLE-STIMULATING HORMONE AND CHORIONIC GONADOTROPHIN

1962 ◽  
Vol 40 (3_Suppl) ◽  
pp. S132 ◽  
Author(s):  
Arthur Carleton Crooke ◽  
W. R. Butt ◽  
R. Morris ◽  
R. Palmer
Keyword(s):  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Stimulating Hormone

THE EFFECT OF HUMAN GONADOTROPHINS ON THE OVARIES OF RADIOLOGICALLY CASTRATED WOMEN

1964 ◽  
Vol 47 (2) ◽  
pp. 277-284 ◽  
Author(s):  
Arne Lindell
Keyword(s):  
Histological Examination ◽  
Cervical Carcinoma ◽  
Ovarian Function ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Hormone Stimulation ◽  
Low Level ◽  
Stimulating Hormone

ABSTRACT Five cases of irradiated cervical carcinoma have been investigated in order to see whether radiological castration completely suppresses ovarian function. After an interval of 7–8 months, and when the oestrogen and pregnanediol excretion had become stabilised at a low level, gonadotrophic hormones were given. Human pituitary follicle stimulating hormone together with human chorionic gonadotrophin were administered in adequate amounts. No effect whatever was seen on the excretion of the three oestrogen fractions investigated, or of pregnanediol. Excretion of 17-OHCS and 17-KS was also unaffected. Histological examination of the ovaries after conclusion of hormone stimulation showed completely atrophic ovaries. As seen in this investigation, the ovarian function after radiological castration is totally abolished and radiological castration produces the same effect as oophorectomy.

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